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Raman Spectroscopy

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Non Invasive Diagnostic Techniques in Clinical Dermatology

Abstract

Raman spectroscopy, a vibrational spectroscopy technique, provides detailed information about molecular composition and molecular structure. Over the last decade, Raman spectroscopy has emerged to a non-invasive, established clinical research tool that benefits clinical dermatology as well as cosmetic and pharmaceutical product development. Applications range from skin structure analysis, response to environmental stress, diagnosis of skin disorders and skin hydration to penetration of actives for drug delivery and toxicology.

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References

  1. Caspers PJ, Lucassen GW, Carter EA, Bruining HA, Puppels GJ (2001) In vivo confocal Raman microspectroscopy of the skin: noninvasive determination of molecular concentration profiles. J Invest Dermatol 116(3):434–442

    Article  PubMed  CAS  Google Scholar 

  2. Wolthuis R, Bakker Schut TC, Caspers Peter J, Buschman HPJ, Römer TJ, Bruining HA et al (1999) Raman spectroscopic methods for in vivo and in vitro tissue characterization. In: Mason WT (ed) Fluorescent and luminent probes for biological activity – a practical guide to technology for quantitative real-time analysis, 2nd edn. Academic, London, pp 433–455

    Google Scholar 

  3. Darvin ME, Lademann J (2010) Resonance Raman spectroscopy of human skin for the in vivo detection of carotenoid antioxidant substances. In: Tuchin VV (ed) Handbook of photonics for biomedical science. CRC Press, Boca Raton, pp 229–252

    Chapter  Google Scholar 

  4. Ermakov IV, Sharifzadeh M, Ermakova M, Gellermann W (2005) Resonance Raman detection of carotenoid antioxidants in living human tissue. J Biomed Opt 10(6):064028, 1–18

    Article  PubMed  Google Scholar 

  5. Williams AC, Barry BW (1999) Raman spectroscopy. In: Bronaugh RL, Maibach HI (eds) Percutaneous absorption drugs-cosmetics-mechanisms-methodology, 3rd edn. Marcel Dekker, New York, pp 499–514

    Google Scholar 

  6. Crowther JM, Sieg A, Blenkiron P, Marcott C, Matts PJ, Kaczvinsky JR et al (2008) Measuring the effects of topical moisturizers on changes in stratum corneum thickness, water gradients and hydration in vivo. Br J Dermatol 159(3):567–577

    PubMed  CAS  Google Scholar 

  7. Caspers PJ, Lucassen GW, Puppels GJ (2003) Combined in vivo confocal Raman spectroscopy and confocal microscopy of human skin. Biophys J 85(1):572–580

    Article  PubMed  CAS  Google Scholar 

  8. Everall N (2004) Depth profiling with confocal Raman microscopy, part II. Spectrosc (Duluth, MN, United States) 19(11):16, 8 ,20–26

    CAS  Google Scholar 

  9. Everall N (2004) Depth profiling with confocal Raman microscopy, part I. Spectrosc (Duluth, MN, United States) 19(10):22, 4–8

    CAS  Google Scholar 

  10. De Faria DLA, De Souza MA (1999) Raman spectra of human skin and nail excited in the visible region. J Raman Spectrosc 30(3):169–171

    Article  Google Scholar 

  11. Gniadecka M, Nielsen OF, Christensen DH, Wulf HC (1998) Structure of water, proteins, and lipids in intact human skin, hair, and nail. J Invest Dermatol 110(4):393–398

    Article  PubMed  CAS  Google Scholar 

  12. Williams AC, Edwards HGM, Barry BW (1994) Raman spectra of human keratotic biopolymers: skin, callus, hair and nail. J Raman Spectrosc 25(1):95–98

    Article  CAS  Google Scholar 

  13. Barry BW, Edwards HGM, Williams AC (1992) Fourier transform Raman and infrared vibrational study of human skin: assignment of spectral bands. J Raman Spectrosc 23(11):641–645

    Article  CAS  Google Scholar 

  14. Guillard E, Tfayli A, Manfait M, Baillet-Guffroy A (2011) Thermal dependence of Raman descriptors of ceramides. Part II: effect of chains lengths and head group structures. Anal Bioanal Chem 399(3):1201–1213

    Article  PubMed  CAS  Google Scholar 

  15. Kessner D, Brezesinski G, Funari SS, Dobner B, Neubert RHH (2010) Impact of the long chain ω-acylceramides on the stratum corneum lipid nanostructure. Part 1: Thermotropic phase behaviour of CER[EOS] and CER[EOP] studied using X-ray powder diffraction and FT-Raman spectroscopy. Chem Phys Lipids 163(1):42–50

    Article  PubMed  CAS  Google Scholar 

  16. Wartewig S, Neubert RHH (2007) Properties of ceramides and their impact on the stratum corneum structure. Skin Pharmacol Physiol 20(5):220–229

    Article  PubMed  CAS  Google Scholar 

  17. Williams AC, Edwards HGM, Lawson EE, Barry BW (2006) Molecular interactions between the penetration enhancer 1,8-cineole and human skin. J Raman Spectrosc 37(1–3):361–366

    Article  CAS  Google Scholar 

  18. Vavrova K, Zbytovska J, Hrabalek A (2005) Amphiphilic transdermal permeation enhancers: structure-activity relationships. Curr Med Chem 12(19):2273–2291

    Article  PubMed  CAS  Google Scholar 

  19. Edwards HGM, Williams AC, Barry BW (1996) Human skin. A Fourier transform Raman spectroscopic study of the Iceman. Spectrosc Eur 8(1):10, 2, 4–6, 8

    CAS  Google Scholar 

  20. Darvin ME, Fluhr JW, Caspers P, van der Pool A, Richter H, Patzelt A et al (2009) In vivo distribution of carotenoids in different anatomical locations of human skin: comparative assessment with two different Raman spectroscopy methods. Exp Dermatol 18(12):1060–1063

    Article  PubMed  CAS  Google Scholar 

  21. Lademann J, Caspers PJ, van der Pol A, Richter H, Patzelt A, Zastrow L et al (2009) In vivo Raman spectroscopy detects increased epidermal antioxidative potential with topically applied carotenoids. Laser Phys Lett 6(1):76–79

    Article  CAS  Google Scholar 

  22. Hata TR, Scholz TA, Ermakov IV, McClane RW, Khachik F, Gellermann W et al (2000) Non-invasive Raman spectroscopic detection of carotenoids in human skin. J Invest Dermatol 115(3):441–448

    Article  PubMed  CAS  Google Scholar 

  23. Egawa M, Nomura J, Iwaki H (2010) The evaluation of the amount of cis- and trans-urocanic acid in the stratum corneum by Raman spectroscopy. Photochem Photobiol Sci 9(5):730–733

    Article  PubMed  CAS  Google Scholar 

  24. Egawa M, Hirao T, Takahashi M (2007) In vivo estimation of stratum corneum thickness from water concentration profiles obtained with Raman spectroscopy. Acta dermato-venereologica 87(1):4–8

    Article  PubMed  Google Scholar 

  25. Crowther JM, Blenkiron P, Matts PJ, Sieg A, Inventors (2007) The Procter & Gamble Company, USA. assignee. Confocal Raman spectroscopy for dermatological studies. Application: WO WO patent 2006-IB53044 2007026325. 20060831

    Google Scholar 

  26. Bielfeldt S, Schoder V, Ely U, van der Pol A, de Sterke J, Wilhelm K-P (2009) Assessment of human stratum corneum thickness and its barrier properties by in-vivo confocal Raman spectroscopy. IFSCC Mag 12(1):9–15

    CAS  Google Scholar 

  27. Zhang Shuliang L, Meyers CL, Subramanyan K, Hancewicz TM (2005) Near infrared imaging for measuring and visualizing skin hydration. A comparison with visual assessment and electrical methods. J Biomed Opt 10(3):031107

    Article  PubMed  CAS  Google Scholar 

  28. Stamatas GN, de Sterke J, Hauser M, von Stetten O, van der Pol A (2008) Lipid uptake and skin occlusion following topical application of oils on adult and infant skin. J Dermatol Sci 50(2):135–142

    Article  PubMed  CAS  Google Scholar 

  29. Matts PJ, Rawlings AV (2006) The dry skin cycle. Cosmetic science and technology series. In: Draelos ZD and Thanan LA (eds) Cosmetic formulation of skin care products, 30. pp 79–114

    Google Scholar 

  30. Chrit L, Bastien P, Biatry B, Simonnet JT, Potter A, Minondo AM et al (2007) In vitro and in vivo confocal Raman study of human skin hydration: assessment of a new moisturizing agent, pMPC. Biopolymers 85(4):359–369

    Article  PubMed  CAS  Google Scholar 

  31. Egawa M, Tagami H (2008) Comparison of the depth profiles of water and water-binding substances in the stratum corneum determined in vivo by Raman spectroscopy between the cheek and volar forearm skin: effects of age, seasonal changes and artificial forced hydration. Br J Dermatol 158(2):251–260

    Article  PubMed  CAS  Google Scholar 

  32. Zhang Q, Andrew Chan KL, Zhang G, Gillece T, Senak L, Moore David J et al (2011) Raman microspectroscopic and dynamic vapor sorption characterization of hydration in collagen and dermal tissue. Biopolymers 95(9):607–615

    Article  PubMed  CAS  Google Scholar 

  33. Edwards HGM, Williams AC, Barry BW (1995) Potential applications of FT-Raman spectroscopy for dermatological diagnostics. J Mol Struct 347:379–387

    Article  CAS  Google Scholar 

  34. Osada M, Gniadecka M, Wulf HC (2004) Near-infrared Fourier transform Raman spectroscopic analysis of proteins, water and lipids in intact normal stratum corneum and psoriasis scales. Exp Dermatol 13(6):391–395

    Article  PubMed  CAS  Google Scholar 

  35. Bernard G, Auger M, Soucy J, Pouliot R (2007) Physical characterization of the stratum corneum of an in vitro psoriatic skin model by ATR-FTIR and Raman spectroscopies. Biochim Biophys Acta (General Subjects) 1770(9):1317–1323

    Article  CAS  Google Scholar 

  36. Wohlrab J, Vollmann A, Wartewig S, Marsch WC, Neubert R (2001) Noninvasive characterization of human stratum corneum of undiseased skin of patients with atopic dermatitis and psoriasis as studied by Fourier transform Raman spectroscopy. Biopolymers 62(3):141–146

    Article  PubMed  CAS  Google Scholar 

  37. O’Regan GM, Kemperman PMJH, Sandilands A, Chen H, Campbell LE, Kroboth K et al (2010) Raman profiles of the stratum corneum define 3 filaggrin genotype-determined atopic dermatitis endophenotypes. J Allergy Clin Immunol 126(3):574–580, e/1

    Article  PubMed  Google Scholar 

  38. Moncada B, Sahagun-Sanchez LK, Torres-Alvares B, Castanedo-Cazares JP, Martinez-Ramirez JD, Gonzalez FJ (2009) Molecular structure and concentration of melanin in the stratum corneum of patients with melasma. Photodermatol Photoimmunol Photomed 25(3):159–160

    Article  PubMed  CAS  Google Scholar 

  39. Azrad E, Cagnano E, Halevy S, Rosenwaks S, Bar I (2005) Bullous pemphigoid detection by micro-Raman spectroscopy and cluster analysis: structure alterations of proteins. J Raman Spectrosc 36(11):1034–1039

    Article  CAS  Google Scholar 

  40. Gniadecka M, Nielsen OF, Wulf HC (2003) Water content and structure in malignant and benign skin tumours. J Mol Struct 661–662:405–410

    Article  Google Scholar 

  41. Kezic S (2008) Methods for measuring in-vivo percutaneous absorption in humans. Hum Exp Toxicol 27(4):289–295

    Article  PubMed  CAS  Google Scholar 

  42. Broding Horst C, van der Pol A, de Sterke J, Monse C, Fartasch M, Bruning T (2011) In vivo monitoring of epidermal absorption of hazardous substances by confocal Raman micro-spectroscopy. J Dtsch Dermatol Ges 9(8):618–627

    PubMed  Google Scholar 

  43. Baena JR, Lendl B (2004) Raman spectroscopy in chemical bioanalysis. Curr Opin Chem Biol 8(5):534–539

    Article  PubMed  CAS  Google Scholar 

  44. Saar BG, Contreras-Rojas LR, Xie XS, Guy RH (2011) Imaging drug delivery to skin with stimulated Raman scattering microscopy. Mol Pharm 8(3):969–975

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Science and Technology, Healthcare Industries, Dow Corning Europe SA, Parc Industriel – Zone C, Rue Jules Bordet, Seneffe, B-7180, Belgium

    Anke Sieg PhD

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  1. Anke Sieg PhD
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Correspondence to Anke Sieg PhD .

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Editors and Affiliations

  1. Clinical Dermatology, San Gallicano Dermatological Institute, Rome, Roma, Italy

    Enzo Berardesca

  2. Dermatology, University of California San Francisco, San Francisco, CA, California, USA

    Howard I. Maibach

  3. Proderm, Schenefeld/Hamburg, Germany

    Klaus-Peter Wilhelm

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Sieg, A. (2014). Raman Spectroscopy. In: Berardesca, E., Maibach, H., Wilhelm, KP. (eds) Non Invasive Diagnostic Techniques in Clinical Dermatology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32109-2_19

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  • DOI: https://doi.org/10.1007/978-3-642-32109-2_19

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-32108-5

  • Online ISBN: 978-3-642-32109-2

  • eBook Packages: MedicineMedicine (R0)

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