Abstract
Cyanobacteria play important roles in carbon dioxide absorption on earth and thus the use of cyanobacteria as biocatalysts is of great significance in viewpoint of green chemistry. However, microalgae including cyanobacteria are rarely applied as biocatalysts because of luck of knowledge of controlling enantioselectivities in asymmetric reactions compared to other microbes. Here, we would like to present that knockout of an alcohol dehydrogenase gene of Synechocystis sp. PCC 6803 could change stereoselectivities of asymmetric reduction of ketones from the wild type microalga. Thus, asymmetric reduction of ethyl pyruvate and trifluoroacetophenone by the mutant deleting of sll0990 and sll1825 gene increased the enantioselectivity.
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References
Nakamura K, Matsuda T (2008) In: V Gotor, I Alfonsa, E Garcia-Uradiales (eds.), Asymmetric Organic Synthesis with Enzymes. Wiley—VCH Verlag GmbH & Co. KGaA: Weinheim, pp. 193–228
Nakamura K (2007) In: T Matsuda (ed.), Future Directions in Biocatalysis. Elsevier: Amsterdam, pp. 51–58
Nakamura K, Yamanaka RJ, Chem Soc, Chem Commun (2002) Light Mediated Cofactor Recycling System in Biocatalytic Asymmetric Reduction of Ketone. 1782-1783
Nakamura K, Yamanaka R (2002) Tetrahedron: Asymm. Light-Mediated Regulation of Asymmetric Reduction of Ketones by a Cyanobacterium. 13: 2529–2533
Nakamura Y, Kaneko T, Hirosawa M, Miyajima N, Tabata S (1998) CyanoBase, a Www Database Containing the Complete Nucleotide Sequence of the Genome of Synechocystis sp. strain PCC6803. Nucleic Acids Res. 26: 63–67
Nie Y, Xu Y, Mu XQ, Wang HY, Yang M, Xiao R (2007) Purification, Characterization, Gene Cloning, and Expression of a Novel Alcohol Dehydrogenase with Anti-Prelog Stereospecificity from Candida Parapsilosis. Appl. Environ. Microbiol. 73: 3759–3764
Takemura T, Akiyama K, Umeno N, Tamai Y, Ohta H, Nakamura K (2009) Asymmetric Reduction of a Ketone by Knockout Mutants of a Cyanobacterium. J. Mol. Cat. B-Enzymatic 60: 93–95
Kojima H, Okada A, Takeda S, Nakamura K (2009) Effect of Carbon Dioxide Concentrations on Asymmetric Reduction of Ketones with Plant Cultured Cells. Tetrahedron Letters 50: 7079–7081
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Ohta, H. et al. (2013). Stereochemical Control of Asymmetric Reduction by Deleting an Alcohol Gehydrogenase Gene of a Cyanobacterium. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_155
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DOI: https://doi.org/10.1007/978-3-642-32034-7_155
Publisher Name: Springer, Berlin, Heidelberg
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