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Reconstructed Skin To Create In Vitro Flexible Models Of Skin Aging: New Results And Prospects

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Textbook of Aging Skin

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Abstract

Despite the fact that a fully differentiated and keratinized epidermis can be produced at the air-liquid interface on acellular dermal substrates or even on membranes it appeared necessary in order to investigate adequately skin physiology and skin aging to deal with a full thickness skin construct made with a living dermis which contains fibroblasts. Based on the pioneering work of Bell and coworkers we have developed such a system in our laboratory which allowed us to investigate skin aging with two separate but complementary approaches. One way was to look at modifications occurring in the dermal matrix like collagen glycation which allowed us to propose for the first time a model of skin aging. Another way was to look at the actual actors of the dermis which are the fibroblasts and to study their destiny during aging which led us to show the key evolution of the papillary fibroblast population during skin aging. In addition to these important findings we have created a skin model for dermal filler studies and we are on the way to create ethnic specific models of skin in vitro.

Combining several parameters like the type of the fibroblast population, the age of the donors, the glycation status, and UV exposure, we now look forward to obtaining more complex but more realistic models of skin aging closer to the in vivo situation. An example of this is shown in which the glycation status, the nature of the fibroblast population used, and the age of the donors were combined.

Moreover, based on a new way to prepare dermal equivalents, we show that it becomes possible to make them with more than two layers which allows to add hypodermis to dermis in our system. Finally in order to escape from the current flat dermal-epidermal junction of our reconstructed skin this method allows us to start investigating the possibility to create reliefs like rete ridge-like or wrinkle-like structures.

Dedication

In Memoriam Jean-François Grollier (1944–2015), L’Oréal former Vice-President and General Director Research and Development.

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Author information

Authors and Affiliations

  1. L’Oréal Recherche et Innovation, 1 ave Eugene Schueller, 93 600, Aulnay sous Bois, France

    Daniel Asselineau, Sylvie Ricois, Hervé Pageon, Hélène Zucchi, Sarah Girardeau-Hubert, Céline Deneuville, Valerie Haydont & Véronique Neiveyans

  2. Centre LOEX de l’Université Laval, 1401, 18è rue, Québec, QC, Canada

    Isabelle Lorthois

Authors
  1. Daniel Asselineau
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  2. Sylvie Ricois
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  3. Hervé Pageon
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  4. Hélène Zucchi
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  5. Sarah Girardeau-Hubert
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  6. Céline Deneuville
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  7. Valerie Haydont
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  8. Véronique Neiveyans
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  9. Isabelle Lorthois
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Corresponding author

Correspondence to Daniel Asselineau .

Editor information

Editors and Affiliations

  1. Procter & Gamble Co., Cincinnati, Ohio, USA

    Miranda A. Farage

  2. Procter & Gamble Co., Cincinnati, Ohio, USA

    Kenneth W. Miller

  3. Department Dermatology, University of California, San Francisco School of Medicine, San Francisco, California, USA

    Howard I. Maibach

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Asselineau, D. et al. (2015). Reconstructed Skin To Create In Vitro Flexible Models Of Skin Aging: New Results And Prospects. In: Farage, M., Miller, K., Maibach, H. (eds) Textbook of Aging Skin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27814-3_48-2

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  • DOI: https://doi.org/10.1007/978-3-642-27814-3_48-2

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  • Online ISBN: 978-3-642-27814-3

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