Abstract
The gut technique of in situ-perfused isolated intestinal segments in rats is used to determine the rates of intestinal absorption or intestinal secretion of a candidate compound, as well as in combination with a bile fistula to detect the hepatobiliary elimination of a candidate compound. By perfusion of different intestinal segments (duodenum, proximal and distal part of the jejunum, and ileum), the site of intestinal absorption or secretion of a candidate compound can be determined.
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References and Further Reading
Arts IC, Sesink AL, Faassen-Peters M, Hollman PC (2004) The type of sugar moiety is a major determinant of the small intestinal uptake and subsequent biliary excretion of dietary quercetin glycosides. Br J Nutr 91:841–847
Berggren S, Hoogstraate J, Fagerholm U, Lennernas H (2004) Characterization of jejunal absorption and apical efflux of ropivacaine, lidocaine and bupivacaine in the rat using in situ and in vitro absorption models. Eur J Pharm Sci 21:553–560
Chaib S, Charrueau C, Neveux N, Coudray-Lucas C, Cynober L, De Bandt JP (2004) Isolated perfused liver model: the rat and guinea pig compared. Nutrition 20:458–464
DeGraw RT, Anderson BD (2004) Determination of carrier-mediated transport of 2′,3′-dideoxypurine nucleosides in the rat ileum using a bidirectional perfusion technique. Pharm Res 21:300–308
den Butter G, Marsh DC, Lindell SL, Belzer FO, Southard JH (1994) Effect of glycine on isolated, perfused rabbit livers following 48-hour preservation in University of Wisconsin solution without glutathione. Transpl Int 7:195–200
Ehehalt R, Jochims C, Lehmann WD, Erben G, Staffer S, Reininger C, Stremmel W (2004) Evidence of luminal phosphatidylcholine secretion in rat ileum. Biochim Biophys Acta 1682:63–71
Gan G, Cartier LL, Huang Y, Yang Z, Sawchuk RJ (2002) Intestinal absorption and presystemic elimination of the prokinetic agent, EM574, in the rabbit. J Pharm Sci 91(1):217–228
Herling AW, Burger HJ, Schwab D, Hemmerle H, Below P, Schubert G (1998) Pharmacodynamic profile of a novel inhibitor of the hepatic glucose-6-phosphatase system. Am J Physiol 274:G1087–G1093
Karpf DM, Kirkegaard AL, Evans AM, Nation RL, Hayball PJ, Milne RW (2003) Effect of ketoprofen and its enantiomers on the renal disposition of methotrexate in the isolated perfused rat kidney. J Pharm Pharmacol 55:1641–1646
Lopez CH, Bracht A, Yamamoto NS, dos Santos MD (1998) Metabolic effects and distribution space of flufenamic acid in the isolated perfused rat liver. Chem Biol Interact 116:105–122
Merino G, Molina AJ, Garcia JL, Pulido MM, Prieto JG, Alvarez AI (2003) Ginseng increases intestinal elimination of albendazole sulfoxide in the rat. Comp Biochem Physiol C Toxicol Pharmacol 136:9–15
Milne RW, Jensen RH, Larsen C, Evans AM, Nation RL (1997) Comparison of the disposition of hepatically-generated morphine-3-glucuronide and morphine-6-glucuronide in isolated perfused liver from the guinea pig. Pharm Res 14:1014–1018
Milne RW, Larsen LA, Jorgensen KL, Bastlund J, Stretch GR, Evans AM (2000) Hepatic disposition of fexofenadine: influence of the transport inhibitors erythromycin and dibromosulphothalein. Pharm Res 17:1511–1515
Poelma FG, Breas R, Tukker JJ, Crommelin DJ (1992) Intestinal absorption of drugs. The influence of mixed micelles on the disappearance kinetics of drugs from the small intestine of the rat. J Pharm Pharmacol 43:317–324
Riad LE, Sawchuk RJ (1991) Absorptive clearance of carbamazepine and selected metabolites in rabbit intestine. Pharm Res 8:1050–1055
Sawchuk RJ, Awni WM (1986) Absorption of cyclosporine from rabbit small intestine in situ. J Pharm Sci 75:1151–1156
Stumpel F, Jungermann K (1997) Sensing by intrahepatic muscarinic nerves of a portal-arterial glucose concentration gradient as a signal for insulin-dependent glucose uptake in the perfused rat liver. FEBS Lett 406:119–122
Stumpel F, Scholtka B, Jungermann K (2000) Stimulation by portal insulin of intestinal glucose absorption via hepatoenteral nerves and prostaglandin E2 in the isolated, jointly perfused small intestine and liver of the rat. Ann N Y Acad Sci 915:111–116
Tsutsumi K, Li SK, Ghanem AH, Ho NF, Higuchi WI (2003) A systematic examination of the in vitro Ussing chamber and the in situ single-pass perfusion model systems in rat ileum permeation of model solutes. J Pharm Sci 92:344–359
Vuppugalla R, Mehvar R (2004) Hepatic disposition and effects of nitric oxide donors: rapid and concentration-dependent reduction in the cytochrome P450-mediated drug metabolism in isolated perfused rat livers. J Pharmacol Exp Ther 310(2):718–727
Wang XD, Russell RM, Marini RP, Tang G, Dolnikowski GG, Fox JG, Krinsky NI (1993) Intestinal perfusion of beta-carotene in the ferret raises retinoic acid level in blood. Biochim Biophys Acta 1167:159–164
Wang J, Nation RL, Evans AM, Cox S (2004a) Isolated rat kidney perfused with dextran and bovine serum albumin: a stable model for investigating renal drug handling. J Pharmacol Toxicol Methods 49:105–113
Wang J, Nation RL, Evans AM, Cox S (2004b) Renal excretion mechanisms of the antiviral nucleoside analogue AM 188 IN the rat isolated perfused kidney. Clin Exp Pharmacol Physiol 31:29–34
Wolkoff AW, Johansen KL, Goeser T (1987) The isolated perfused rat liver: preparation and application. Anal Biochem 167:1–14
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Herling, A.W. (2013). Distribution – In Vivo – Perfused Organs. In: Vogel, H.G., Maas, J., Hock, F.J., Mayer, D. (eds) Drug Discovery and Evaluation: Safety and Pharmacokinetic Assays. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-25240-2_40
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DOI: https://doi.org/10.1007/978-3-642-25240-2_40
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-25239-6
Online ISBN: 978-3-642-25240-2
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