Definition
High-resolution AFM topography imaging becomes a powerful bioanalytic tool when combined with single-molecule force spectroscopy or with simultaneous “topography and recognition imaging” (TREC). This new imaging method allows for mapping of specific ligand binding sites on biological samples under physiological conditions with nanometer resolution.
Introduction
Atomic force microcopy (AFM) is a version member of scanning probe microscopy (SPM) that is extensively used in solid-state physics and materials science within the life sciences because it can be operated in physiological salt solution. In AFM imaging, a sharp probe tip mounted on a microcantilever scans over the specimen line by line, whereby the topographic image of the sample surface is generated by “feeling” rather than “looking.” Tips with high sharpness provide high resolution and cantilevers with low spring constants allow...
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Duman, M. et al. (2013). Atomic Force Microscopy (AFM) for Topography and Recognition Imaging at Single Molecule Level. In: Roberts, G.C.K. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16712-6_496
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DOI: https://doi.org/10.1007/978-3-642-16712-6_496
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-16711-9
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