Skip to main content

Porous Silicon Electrochemical Biosensors: Basic Principles and Detection Strategies

  • Reference work entry
  • First Online:
Handbook of Porous Silicon

Abstract

Although electrochemical transducers are attractive options in the field of biosensing due to their practicability, simplicity, low power requirements, and low cost, porous silicon electrochemical biosensors have not received as much attention as optical ones. In this article, we conduct a comprehensive review of the applications of porous silicon in electrochemical biosensing mainly based on the four types of transduction, namely, potentiometric, impedimetric, amperometric, and conductometric methods. Nowadays, porous silicon advantages such as large surface area, high tunable porosity, and biocompatibility are the main factors to develop new approaches for porous silicon functionalization, derivatization, and oxidation as well as porous silicon nanocomposites preparation. These are leading to the realization of advanced porous silicon-based electrochemical biosensors.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 849.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 549.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Archer M, Fauchet PM (2003) Electrical sensing of DNA hybridization in porous silicon layers. Phys Status Solidi 198:503

    Article  CAS  Google Scholar 

  • Archer M, Christophersen M, Fauchet PM (2004) Macroporous silicon electrical sensor for DNA hybridization detection. Biomed Microdevices 6:203

    Article  CAS  Google Scholar 

  • Bahadir EB, Sezginturk MK (2016) Electrochemical biosensors for hormone analyses. Biosens Bioelectron 68:62

    Article  CAS  Google Scholar 

  • Basu I, Subramanian RV, Mathew A, Kayastha AM, Chadha A, Bhattacharya E (2005) Solid state potentiometric sensor for the estimation of tributyrin and urea. Sens Actuator B-Chem 107:418

    Article  CAS  Google Scholar 

  • Betty CA (2009) Highly sensitive capacitive immunosensor based on porous silicon–polyaniline structure: bias dependence on specificity. Biosens Bioelectron 25:338

    Article  CAS  Google Scholar 

  • Betty A, Lal R, Sharma DK, Yakhmi JV, Mittal JP (2004) Macroporous silicon based capacitive affinity sensor-fabrication and electrochemical studies. Sens Actuator B 97:334

    Article  CAS  Google Scholar 

  • Canham LT (1990) Silicon quantum wire array fabricated by electrochemical and chemical dissolution of wafers. Appl Phys Lett 57:1046

    Article  CAS  Google Scholar 

  • Clark LC, Lyons C (1962) Electrode systems for continuous monitoring in cardiovascular surgery. Ann N Y Acad Sci 102:29

    Article  CAS  Google Scholar 

  • Das RD, Chaudhuri CR, Maji S, Das S, Saha H (2009) Macroporous silicon-based simple and efficient trapping platform for electrical detection of Salmonella typhimurium pathogens. Biosens Bioelectron 24:3215

    Article  CAS  Google Scholar 

  • Das RD, Dey A, Das S, Chaudhuri CR (2011) Interdigitated electrode-less high-performance macroporous silicon structure as impedance biosensor for bacteria detection. IEEE Sens J 11:1242

    Article  CAS  Google Scholar 

  • Das RD, Mondal N, Das S, Chaudhuri CR (2012) Optimized electrode geometry for an improved impedance based macroporous silicon bacteria detector. IEEE Sens J 12:1868

    Article  Google Scholar 

  • Ensafi AA, Abarghoui MM, Rezaei B (2014a) A new non-enzymatic glucose sensor based on copper/porous silicon nanocomposite. Electrochim Acta 123:219

    Article  CAS  Google Scholar 

  • Ensafi AA, Abarghoui MM, Rezaei B (2014b) Electrochemical determination of hydrogen peroxide using copper/porous silicon-based non-enzymatic sensor. Sens Actuators B 196:398

    Article  CAS  Google Scholar 

  • Ensafi AA, Abarghoui MM, Rezaei B (2014c) A new electrochemical sensor based on porous silicon supported Pt-Pd nanoalloy for simultaneous determination of adenine and guanine. Sens Actuator B 204:528

    Article  CAS  Google Scholar 

  • Ensafi AA, Abarghoui MM, Rezaei B (2015a) Simultaneous determination of morphine and codeine using Pt nanoparticles supported on porous silicon flour modified ionic liquid carbon paste electrode. Sens Actuator B 219:1

    Article  CAS  Google Scholar 

  • Ensafi AA, Ahmadi N, Rezaei B, Abarghoui MM (2015b) A new electrochemical sensor for the simultaneous determination of acetaminophen and codeine based on porous silicon/palladium nanostructure. Talanta 134:745

    Article  CAS  Google Scholar 

  • Ensafi AA, Abarghoui MM, Rezaei B (2016) Facile synthesis of Pt-Cu@silicon nanostructure as a new electrocatalyst supported matrix, electrochemical detection of hydrazine and hydrogen peroxide. Electrochim Acta 190:199

    Article  CAS  Google Scholar 

  • Fernandez RE, Bhattacharya E, Chadha A (2008) Covalent immobilization of Pseudomonas cepacia lipase on semiconducting materials. Appl Surf Sci 254:4512

    Article  CAS  Google Scholar 

  • Fernandez RE, Hareesh V, Bhattacharya E, Chadha A (2009) Comparison of a potentiometric and a micromechanical triglyceride biosensor. Biosens Bioelectron 24:1276

    Article  CAS  Google Scholar 

  • Ghosh H, Chaudhuri CR (2013) Ultrasensitive food toxin biosensor using frequency based signals of silicon oxide nanoporous structure. Appl Phys Lett 102:243701

    Article  CAS  Google Scholar 

  • Guan JG, Miao YQ, Zhang QJ (2004) Impedimetric biosensors. J Biosci Bioeng 97:219

    Article  CAS  Google Scholar 

  • Jane A, Dronov R, Hodges A, Voelcker NH (2009) Porous silicon biosensors on the advance. Trends Biotechnol 27:230

    Article  CAS  Google Scholar 

  • Jin JH, Zhang D, Alocilja EC (2008) Label-free DNA sensor on nanoporous silicon-polypyrrole chip for monitoring salmonella species. IEEE Sens J 8:891

    Article  CAS  Google Scholar 

  • Jin JH, Hong SI, Min NK (2009) Integrated urea sensor module based on poly(3-methylthiophene)-modified p-type porous silicon substrate. J Porous Mater 16:379

    Article  CAS  Google Scholar 

  • Jin JH, Alocilja EC, Grooms DL (2010) Fabrication and electroanalytical characterization of label-free DNA sensor based on direct electropolymerization of pyrrole on p-type porous silicon substrates. J Porous Mater 17:169

    Article  CAS  Google Scholar 

  • Kimmel DW, LeBlanc G, Meschievitz ME, Cliffel DE (2012) Electrochemical sensors and biosensors. Anal Chem 84:685

    Article  CAS  Google Scholar 

  • Kumari V, Mishra P, Islam SS (2012) Development and standardization of porous silicon for application as a working electrode in electrochemical immunosensor. In: 16th international workshop on physics of semiconductor devices 8549:85491I

    Google Scholar 

  • Li H, Wang Q, Wei Q, Chen L (2011) Study on urea-sensitive electrode based porous silicon. Adv Mat Res 239:654

    Google Scholar 

  • Lopez-Garcia J, Martin-Palma RJ, Manso M, Martinez-Duart JM (2007) Porous silicon based structures for the electrical biosensing of glucose. Sens Actuator B 126:82

    Article  CAS  Google Scholar 

  • Marks RS, Lowe CR, Cullen DC, Weetall HH, Karube I (2007) Handbook of biosensors and biochips. Wiley, New York

    Google Scholar 

  • Mathew A, Pandian G, Bhattacharya E, Chadha A (2009) Novel applications of silicon and porous silicon based EISCAP biosensors. Phys Status Solidi (a) 206:1369

    Article  CAS  Google Scholar 

  • Melikjanyan GA, Martirosyan KS (2011) Possibility of application of porous silicon as glucose biosensor. Armen J Phys 4:225

    CAS  Google Scholar 

  • Nath N, Chilkoti A (2002) A colorimetric gold nanoparticle sensor to interrogate biomolecular interactions in real time on a surface. Anal Chem 74:504

    Article  CAS  Google Scholar 

  • Prabhakar D, Kumari V, Islam SS (2012) Nanoporous silicon based electrochemical immunosensor. Sci Adv Mater 4:121

    Article  CAS  Google Scholar 

  • Preetha R, Rani K, Veeramani MSS, Fernandez RE, Vemulachedu H, Sugan M, Bhattacharya E, Chadha A (2011) Potentiometric estimation of blood analytes-triglycerides and urea: comparison with clinical data and estimation of urea in milk using an electrolyte–insulator–semiconductor–capacitor (EISCAP). Sens Actuator B 160:1439

    Article  CAS  Google Scholar 

  • Privett BJ, Shin JH, Schoenfisch MH (2010) Electrochemical sensors. Anal Chem 82:4723

    Article  CAS  Google Scholar 

  • Ravindra NM, Prodan C (2007) Advances in the manufacturing types and applications of biosensors. JOM 59:37

    Article  CAS  Google Scholar 

  • Recio-Sanchez G, Torres-Costa V, Manso M, Gallach D, Lopez-Garcia J, Martín-Palma RJ (2010) Towards the development of electrical biosensors based on nanostructured porous silicon. Materials 3:755

    Article  CAS  Google Scholar 

  • Reddy RRK, Chadha A, Bhattacharya E (2001) Porous silicon based potentiometric triglyceride biosensor. Biosens Bioelectron 16:313

    Article  CAS  Google Scholar 

  • Reddy RRK, Basu I, Bhattacharya E, Chadha A (2003) Estimation of triglycerides by a porous silicon based potentiometric biosensor. Curr Appl Phys 3:155

    Article  Google Scholar 

  • Reta N, Michelmore A, Saint C, Prieto-Simon B, Voelcker NH (2016) Porous silicon membrane-modified electrodes for label-free voltammetric detection of MS2 bacteriophage. Biosens Bioelectron 80:47

    Article  CAS  Google Scholar 

  • Scheller FW, Wollenberger U, Warsinke A, Lisdat F (2001) Research and development in biosensors. Curr Opin Biotechnol 12:35

    Article  CAS  Google Scholar 

  • Schoning MJ, Ronkel F, Crott M, Thust M, Schultzen JW, Kordos P, Luth H (1997) Miniaturization of potentiometric using porous silicon microtechnology. Electrochem Acta 42:3185

    Article  CAS  Google Scholar 

  • Setzu S, Salis S, Demontis V, Salis A, Monduzzi M, Mula G (2007) Porous silicon-based potentiometric biosensor for triglycerides. Phys Status Solidi (a) 204:1434

    Article  CAS  Google Scholar 

  • Song MJ, Yun DH, Min NK, Hong SI (2007) Electrochemical biosensor array for liver diagnosis using silanization technique on nanoporous silicon electrode. J Biosci Bioeng 103:32

    Article  CAS  Google Scholar 

  • Song MJ, Yun DH, Hong SI (2009) An electrochemical biosensor array for rapid detection of alanine aminotransferase and aspartate aminotransferase. Biosci Biotechnol Biochem 73:474

    Article  CAS  Google Scholar 

  • Stetter JR, Penrose WR, Yao S (2003) Sensors, chemical sensors, electrochemical sensors, and ECS. J Electrochem Soc 150:11

    Article  CAS  Google Scholar 

  • Sweetman MJ, Shearer CJ, Shapter JG, Voelcker NH (2011) Dual silane surface functionalization for the selective attachment of human neuronal cells to porous silicon. Langmuir 27:9497

    Article  CAS  Google Scholar 

  • Tembe S, Chaudhari PS, Bhoraskar SV, D’Souza SF, Karve MS (2008) Conductivity-based catechol sensor using tyrosinase immobilized in porous silicon. IEEE Sens J 9:1593

    Article  CAS  Google Scholar 

  • Thust M, Schoning MJ, Frohnhoff S, Arens-Fischer R, Kordos P, Luth H (1996) Porous silicon as a substrate material for potentiometric biosensors. Meas Sci Technol 7:26

    Article  CAS  Google Scholar 

  • Turner DC, Chang CY, Fang K, Brandow SL, Murphy DB (1995) Selective adhesion of functional microtubules to patterned silane surfaces. Biophys J 69:2782

    Article  CAS  Google Scholar 

  • Uhlir A (1956) Electrolytic shaping of germanium and silicon. Bell Syst Tech J 35:333

    Article  CAS  Google Scholar 

  • Vamvakaki V, Chaniotakis NA (2008) DNA stabilization and hybridization detection on porous silicon surface by EIS and total reflection FT-IR spectroscopy. Electroanalysis 20:1845

    Article  CAS  Google Scholar 

  • Yoon JY (2013) Introduction to biosensors, from electric circuits to immunosensors. Springer, Berlin/Heidelberg

    Google Scholar 

  • Yun D, Song MJ, Hwang S, Hong SI (2012) Fabrication and electrochemical characterization of nanoporous silicon electrode for amperometric urea biosensor. Jpn J Appl Phys 51:06FG02

    Article  Google Scholar 

  • Zhang D, Alocilja EC (2008) Characterization of nanoporous silicon-based DNA biosensor for the detection of salmonella enteritidis. IEEE Sens J 8:775

    Article  CAS  Google Scholar 

  • Zhu C, Yang G, Li H, Du D, Lin Y (2015) Electrochemical sensors and biosensors based on nanomaterials and nanostructures. Anal Chem 87:230

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ali Asghar Ensafi .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer International Publishing AG, part of Springer Nature

About this entry

Check for updates. Verify currency and authenticity via CrossMark

Cite this entry

Ensafi, A.A., Abarghoui, M.M., Ahmadi, N. (2018). Porous Silicon Electrochemical Biosensors: Basic Principles and Detection Strategies. In: Canham, L. (eds) Handbook of Porous Silicon. Springer, Cham. https://doi.org/10.1007/978-3-319-71381-6_112

Download citation

Publish with us

Policies and ethics