Abstract
The hematoxylin and eosin stain (H&E stain) is one of the most popular, routine stain in histology. It allows to observe an overall cellular architecture of tissue and is more versatile than such stainings as immunohistochemistry. In this paper, we evaluate performance of state-of-the-art methods for stain unmixing in H&E-stained images. The aim of our study is to measure the degree of conformity of relative stain concentration maps obtained automatically to ground truth, to examine their robustness to variations in staining and illumination between images, and to assess their usefulness for routine usage.
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This work was supported by AGH University of Science and Technology research grant No. 11.11.120.612.
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Kłeczek, P., Mól, S., Jaworek-Korjakowska, J. (2018). The Accuracy of H&E Stain Unmixing Techniques When Estimating Relative Stain Concentrations. In: Augustyniak, P., Maniewski, R., Tadeusiewicz, R. (eds) Recent Developments and Achievements in Biocybernetics and Biomedical Engineering. PCBBE 2017. Advances in Intelligent Systems and Computing, vol 647. Springer, Cham. https://doi.org/10.1007/978-3-319-66905-2_7
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DOI: https://doi.org/10.1007/978-3-319-66905-2_7
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