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Proteomic Biomarkers for Endometriosis

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Biomarkers for Endometriosis

Abstract

Endometriosis is a benign, estrogen-dependent gynecology disorder associated with pelvic pain and infertility. It is characterized by the presence of endometrial-like tissue outside the uterine cavity, mainly on the pelvic peritoneum and ovaries and in the rectovaginal septum and more rarely in the pericardium, the pleura, and even the brain. The etiology and pathogenesis remains unclear. The most accepted theory is Sampson’s theory: retrograde menstruation. The gold standard of diagnosing endometriosis is through laparoscopy.

Proteomics research has found differentially expressed protein/peptides; however, till today we have not found a non- or semi-invasive test for endometriosis. To date, two most commonly applied technologies used in endometriosis research are surfaced-enhanced laser desorption ionization (SELDI)-time-of-flight (TOF) mass spectrometry (MS) and two-dimensional difference gel electrophoresis (2D DIGE). In this chapter we will discuss the proteomics technologies available and their advantages and disadvantages and critically describe the biomarker proteomics results in endometriosis.

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Abbreviations

2D DIGE:

Two-dimensional difference gel electrophoresis

FTMS:

Fourier transform mass spectrometry

HPLC:

High-performance liquid chromatography

ICAT:

Isotope-coded affinity tags

ICPL:

Isotope-coded protein labeling

LC:

Liquid chromatography

MALDI:

Matrix-assisted laser desorption ionization

MS:

Mass spectrometry

MudPIT:

Multidimensional Protein Identification Technology

SCX:

Strong cation exchanger

SELDI:

Surface-enhanced laser desorption ionization

SILAC:

Stable isotope labeling by amino acids in cell culture

TOF:

Time of flight

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Author information

Authors and Affiliations

  1. Department of Development and Regeneration, Organ Systems, KU Leuven, Leuven, Belgium

    Amelie Fassbender Ph.D. & Dorien O

  2. Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, UZ Gasthuisberg, Leuven, 3000, Belgium

    Amelie Fassbender Ph.D. & Dorien O

  3. Department of Development and Regeneration, Organ Systems, Group Biomedical Sciences, KU Leuven (University of Leuven), Leuven, Belgium

    Thomas D’Hooghe M.D., Ph.D.

  4. Global Medical Affairs Fertility, Research and Development, Merck KGaA, Darmstadt, Belgium

    Thomas D’Hooghe M.D., Ph.D.

  5. Department of Cellular and Molecular Medicine, Campus Gasthuisberg, Leuven, Belgium

    Antoine Hubert & Etienne Waelkens

  6. SyBioMa, Interfaculty Centre for Systems Biology based Mass Spectrometry, Campus Gasthuisberg, Leuven, Belgium

    Etienne Waelkens

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  1. Amelie Fassbender Ph.D.
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  2. Antoine Hubert
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  3. Etienne Waelkens
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  4. Dorien O
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  5. Thomas D’Hooghe M.D., Ph.D.
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Correspondence to Thomas D’Hooghe M.D., Ph.D. .

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  1. Department of Development and Regeneration Organ Systems, Group Biomedical Sciences, KU Leuven (University of Leuven), Leuven, Belgium

    Thomas D'Hooghe

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Fassbender, A., Hubert, A., Waelkens, E., O, D., D’Hooghe, T. (2017). Proteomic Biomarkers for Endometriosis. In: D'Hooghe, T. (eds) Biomarkers for Endometriosis. Springer, Cham. https://doi.org/10.1007/978-3-319-59856-7_10

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  • DOI: https://doi.org/10.1007/978-3-319-59856-7_10

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  • Online ISBN: 978-3-319-59856-7

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