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Methodology Part II. Pochonia spp.: Screening and Isolate Selection for Managing Plant-Parasitic Nematodes

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Perspectives in Sustainable Nematode Management Through Pochonia chlamydosporia Applications for Root and Rhizosphere Health

Part of the book series: Sustainability in Plant and Crop Protection ((SUPP))

Abstract

Production of Pochonia spp. in laboratory conditions has facilitated studies on its biology, abundance, dispersion, rhizosphere colonization, host preference, and isolate virulence. Research in biological control of nematodes requires suitable, standardized methods. In this chapter we review the commonest, non-molecular, standard in vitro culture methods to isolate, screen, and select isolates, some of which may eventually be produced on a larger scale for application in combination with other management strategies for plant-parasitic nematodes.

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Authors and Affiliations

  1. CFE-Centre for Functional Ecology, Department of Life Sciences, Calçada Martim de Freitas, University of Coimbra, Coimbra, P3000-456, Portugal

    Ivânia Esteves

  2. UNIGRAS (Unidad de Investigación en Granos y Semillas), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Av. Dr. Jorge Jiménez Cantú s/n, Cuautitlán Izcalli, C.P. 54740, Mexico

    Rosa Navarrete-Maya

  3. Centro de Desarrollo de Productos Bióticos (Visiting Professor), Yautepec, Morelos, C.P. 62731, Mexico

    Rosa H. Manzanilla-López

Authors
  1. Ivânia Esteves
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  2. Rosa Navarrete-Maya
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  3. Rosa H. Manzanilla-López
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Correspondence to Ivânia Esteves .

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Editors and Affiliations

  1. Centro de Desarrollo de Productos Bióticos (Visiting Professor), Instituto Politécnico Nacional (CEPROBI), Yautepec, Morelos, Mexico

    Rosa H. Manzanilla-López

  2. Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain

    Luis V. Lopez-Llorca

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Esteves, I., Navarrete-Maya, R., Manzanilla-López, R.H. (2017). Methodology Part II. Pochonia spp.: Screening and Isolate Selection for Managing Plant-Parasitic Nematodes. In: Manzanilla-López, R., Lopez-Llorca, L. (eds) Perspectives in Sustainable Nematode Management Through Pochonia chlamydosporia Applications for Root and Rhizosphere Health. Sustainability in Plant and Crop Protection. Springer, Cham. https://doi.org/10.1007/978-3-319-59224-4_12

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