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Characterization of Radical S-adenosylmethionine Enzymes and Intermediates in their Reactions by Continuous Wave and Pulse Electron Paramagnetic Resonance Spectroscopies

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Future Directions in Metalloprotein and Metalloenzyme Research

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 33))

Abstract

Radical S-adenosylmethionine (SAM) enzymes comprise an important and a versatile superfamily of enzymes. For more than a decade, a significant effort has been directed towards understanding these enzymes. Electron paramagnetic resonance spectroscopy has played a crucial role in such studies, helping to decipher intricate details about the identity of the active metallocofactors, their relations to the substrate(s) utilized and an understanding of the mechanisms of the enzymatic reactions. In this chapter we overview research milestones in the field of radical SAM enzymes achieved with the aid of EPR spectroscopy.

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Authors and Affiliations

  1. Departments of Chemistry and Biochemistry and Molecular Biology, The Howard Hughes Medical Institute, The Pennsylvania State University, University Park, PA, 16802, USA

    Alexey Silakov, Nicholas D. Lanz & Squire J. Booker

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  1. Alexey Silakov
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  2. Nicholas D. Lanz
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  3. Squire J. Booker
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Correspondence to Alexey Silakov .

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  1. Brisbane, Australia

    Graeme Hanson

  2. Department of Chemistry and Biochemistry, University of Denver, Denver, Colorado, USA

    Lawrence Berliner

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Silakov, A., Lanz, N.D., Booker, S.J. (2017). Characterization of Radical S-adenosylmethionine Enzymes and Intermediates in their Reactions by Continuous Wave and Pulse Electron Paramagnetic Resonance Spectroscopies. In: Hanson, G., Berliner, L. (eds) Future Directions in Metalloprotein and Metalloenzyme Research. Biological Magnetic Resonance, vol 33. Springer, Cham. https://doi.org/10.1007/978-3-319-59100-1_6

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