Skip to main content

Miscellaneous Specimens and Ancillary Techniques

  • Chapter
  • First Online:
Histopathology Specimens
  • 1118 Accesses

Abstract

This chapter refers to miscellaneous specimens—needle core biopsies, fine needle aspirates, cytospins, liquid-based cytology, and cell blocks. Aids to macroscopic description and dissection such as specimen photography and radiography and intraoperative frozen section assessment are considered. Key ancillary techniques now playing an essential role in diagnosis, prognosis, and management decisions are highlighted. These include immunohistochemistry, immunofluorescence, flow cytometry, in situ hybridization, electron microscopy, and molecular genetic analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 139.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 179.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Bibliography

  • Allen DC. Histopathology reporting. Guidelines for surgical cancer. 3rd ed. London: Springer; 2013.

    Book  Google Scholar 

  • Allen DC, Cameron RI. Histopathology specimens: clinical, pathological and laboratory aspects. 2nd ed. Berlin/Heidelberg: Springer; 2013.

    Book  Google Scholar 

  • Bahrami A, Truong LD, Ro JY. Undifferentiated tumor: true identity by immunohistochemistry. Arch Pathol Lab Med. 2008;132:326–48.

    PubMed  Google Scholar 

  • De Kerviler E, Guermazi A, Zagdanski AM, et al. Image-guided core-needle biopsy in patients with suspected or recurrent lymphoma. Cancer. 2000;89:647–52.

    Article  PubMed  Google Scholar 

  • Dodson A. Modern methods for diagnostic immunocytochemistry. Curr Diagn Pathol. 2002;8:113–22.

    Article  Google Scholar 

  • Groenen PJTA, Blokx WAM, Diepenbroek C, Burgers L, Visinoni F, Wesseling P, van Krieken JHJM. Preparing pathology for personalized medicine: possibilities for improvement of the pre-analytical phase. Histopathology. 2011;59:1–7.

    Article  PubMed  Google Scholar 

  • Medical Research Council. Human tissue and biological samples for use in research: operational and ethical guidelines. London: Medical Research Council; 2001. http://www.mrc.ac.uk/pdf-tissue_guide_fin.pdf. Accessed Nov 2002

    Google Scholar 

  • Jasani B, Rhodes A. The role and mechanism of high temperature antigen retrieval in diagnostic pathology. Curr Diagn Pathol. 2001;7:153–60.

    Article  Google Scholar 

  • Kamalakaran S, Varadan V, Janevski A, Banerjee N, Tuck D, McCombie WR, Dimitrova N, Harris LN. Translating next generation sequencing to practice: opportunities and necessary steps. Mol Oncol. 2013;7(4):743–55.

    Article  CAS  PubMed  Google Scholar 

  • Lester SC. Chapter 6: Operating room consultations. In:Manual of surgical pathology. 3rd ed. Philadelphia: Churchill Livingstone; 2007.

    Google Scholar 

  • Matthews TJ, Denney PA. Digital imaging of specimens using a wet scanning technique. J Clin Pathol. 2001;54:326–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • McKay B. Electron microscopy in tumour diagnosis. In: Fletcher CDM, editor. Diagnostic histopathology of tumours, vol. 2. 3rd ed. London: Churchill Livingstone; 2007. p. 1831–59.

    Google Scholar 

  • McManus DT, Anderson NH. Fine Needle Aspiration Cytology of the breast. Curr Diagn Pathol. 2001;7:262–71.

    Article  Google Scholar 

  • Micklem K, Sanderson J. Digital imaging in pathology. Curr Diagn Pathol. 2001;7:131–40.

    Article  Google Scholar 

  • Mount SL, Cooper K. Beware of biotin: a source of false positive immunohistochemistry. Curr Diagn Pathol. 2001;7:161–7.

    Article  Google Scholar 

  • Nocito A, Kononen J, Kallioniemi OP, Sauter G. Tissue microarrays for high-throughput molecular pathology research. Int J Cancer. 2001;94:1–5.

    Article  CAS  PubMed  Google Scholar 

  • Pecciarini L, Giulia Cangi M, Doglioni C. Identifying the primary sites of metastatic carcinoma: the increasing role of immunohistochemistry. Curr Diagn Pathol. 2001;7:168–75.

    Article  Google Scholar 

  • Perou CM, Sorlie T, Eisen MB, et al. Molecular portraits of human breast tumours. Nature. 2000;406:747–52.

    Article  CAS  PubMed  Google Scholar 

  • Shipp MA, Ross KN, Tamayo P, et al. Diffuse large B-cell lymphoma outcome prediction by gene-expression profiling and supervised machine learning. Nat Med. 2002;8:68–74.

    Article  CAS  PubMed  Google Scholar 

  • Stricker T, Catenacci DVT, Seiwert TY. Molecular profiling of cancer – the future of personalized medicine: a primer on cancer biology and the tools necessary to bring molecular testing to the clinic. Semin Oncol. 2011;38:173–85.

    Google Scholar 

  • Swerdlow SH, Compo E, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J, Vardiman JW. WHO classification of tumours pathology and genetics. Tumours of haemopoietic and lymphoid tissues. Lyon: IARC Press; 2008.

    Google Scholar 

  • Teruya-Feldstein J. The immunohistochemistry laboratory: looking at molecules and preparing for tomorrow. Arch Pathol Lab Med. 2010;134:1659–65.

    PubMed  Google Scholar 

  • Walker RA, Bartlett JMS, Dowsett M, Ellis IO, Hanby AM, Jasani B, Miller K, Pinder SE. HER2 testing in the UK: further update to recommendations. J Clin Pathol. 2008;61:818–24.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Damian T. McManus .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Cite this chapter

McManus, D.T. (2017). Miscellaneous Specimens and Ancillary Techniques. In: Allen, D., Cameron, R. (eds) Histopathology Specimens. Springer, Cham. https://doi.org/10.1007/978-3-319-57360-1_46

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-57360-1_46

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-57359-5

  • Online ISBN: 978-3-319-57360-1

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics