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Resistance of Mosquitoes to Entomopathogenic Bacterial-Based Larvicides: Current Status and Strategies for Management

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Bacillus thuringiensis and Lysinibacillus sphaericus

Abstract

The entomopathogenic bacteria Bacillus thuringiensis serovar. israelensis (Bti) and Lysinibacillus sphaericus have successfully been used to control insects of public health relevance, including those from the genera Aedes, Anopheles, Culex, and Simulium. These bacteria display a specific mode of action that relies on unique interactions which makes them the most selective agents currently available to control Diptera larvae. They produce crystalline insecticidal proteins that act on the larval midgut through their interaction with specific receptors. L. sphaericus presents a single major larvicidal factor, the binary (Bin) protoxin, whose action relies on the binding to one class of receptors, while Bti crystals contain four main protoxins (Cry4Aa, Cry4Ba, Cry11Aa, Cyt1Aa) which display interactions with a group of distinct midgut receptor molecules. The mode of action of L. sphaericus displays a greater potential for resistance selection, compared to Bti which has no record of insect resistance to date. These major mosquitocidal toxins and their interaction with midgut target sites, as well as resistance issues related to their utilization, are summarized in this chapter.

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  1. Centro de Pesquisas Aggeu Magalhães-FIOCRUZ, Recife, PE, 50740-465, Brazil

    Maria Helena Neves Lobo Silva-Filha

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  1. Maria Helena Neves Lobo Silva-Filha
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Correspondence to Maria Helena Neves Lobo Silva-Filha .

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  1. Control Agro Bio Pesquisa e Defesa, Agropecuária Ltda, Porto Alegre, Brazil

    Lidia Mariana Fiuza

  2. Plant Protection Department, Universidade Estadual Paulista, Jaboticabal, Brazil

    Ricardo Antonio Polanczyk

  3. School of Life Sciences, University of Sussex, Brighton, United Kingdom

    Neil Crickmore

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Silva-Filha, M.H.N.L. (2017). Resistance of Mosquitoes to Entomopathogenic Bacterial-Based Larvicides: Current Status and Strategies for Management. In: Fiuza, L., Polanczyk, R., Crickmore, N. (eds) Bacillus thuringiensis and Lysinibacillus sphaericus. Springer, Cham. https://doi.org/10.1007/978-3-319-56678-8_15

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