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Combining Isotope Labelling with High Resolution Liquid Chromatography-Tandem Mass Spectrometry to Study Sulfur Amino Acid Metabolism in Seeds of Common Bean (Phaseolus vulgaris)

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Sulfur Metabolism in Higher Plants - Fundamental, Environmental and Agricultural Aspects

Part of the book series: Proceedings of the International Plant Sulfur Workshop ((PIPSW))

Abstract

Sulfur plays a crucial role in plant metabolism, protein biosynthesis, homeostasis and defense mechanisms. Like in other grain legumes, the protein quality of common bean is limited by the sub-optimal concentration of methionine and cysteine. S-methylcysteine is a non-proteinogenic sulfur amino acid, characteristically found in the Phaseolus as well as Vigna species. In mature seeds it accumulates in the form of its dipeptide γ-glutamyl-S-methylcysteine. In human diet, this sulfur containing amino acid and its dipeptide cannot substitute cysteine or methionine. Despite the inverse relationship between the concentration of cysteine and methionine versus S-methylcysteine, to date very little is known about the biosynthesis of the latter in common bean. Here, we developed a method, combining stable isotope label tracking with high resolution liquid chromatography-tandem mass spectrometry to investigate the pathways of S-methylcysteine and γ-glutamyl-S-methylcysteine biosynthesis in the developing seed of common bean.

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Authors and Affiliations

  1. Department of Biology, University of Western Ontario, 1151 Richmond St., London, ON, N6A 3K7, Canada

    Jaya Joshi & Frédéric Marsolais

  2. Genomics and Biotechnology, London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford St., London, ON, N5V 4T3, Canada

    Jaya Joshi, Justin B. Renaud, Mark W. Sumarah & Frédéric Marsolais

Authors
  1. Jaya Joshi
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  2. Justin B. Renaud
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  3. Mark W. Sumarah
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  4. Frédéric Marsolais
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Corresponding author

Correspondence to Frédéric Marsolais .

Editor information

Editors and Affiliations

  1. Laboratory of Plant Physiology, University of Groningen, Groningen, Groningen, The Netherlands

    Luit J. De Kok

  2. Plant Biology and Crop Science Department, Rothamsted Research, Harpenden, Hertfordshire, United Kingdom

    Malcolm J. Hawkesford

  3. Institute for Crop and Soil Science, Julius Kühn Institute (JKI) Institute for Crop and Soil Science, Braunschweig, Germany

    Silvia H. Haneklaus

  4. Crop and Soil Science, Julius Kühn-Institut (JKI), Braunschweig, Germany

    Ewald Schnug

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Joshi, J., Renaud, J.B., Sumarah, M.W., Marsolais, F. (2017). Combining Isotope Labelling with High Resolution Liquid Chromatography-Tandem Mass Spectrometry to Study Sulfur Amino Acid Metabolism in Seeds of Common Bean (Phaseolus vulgaris). In: De Kok, L., Hawkesford, M., Haneklaus, S., Schnug, E. (eds) Sulfur Metabolism in Higher Plants - Fundamental, Environmental and Agricultural Aspects. Proceedings of the International Plant Sulfur Workshop. Springer, Cham. https://doi.org/10.1007/978-3-319-56526-2_13

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