Abstract
Bioactive peptides (BPs) are protein hydrolysates able to induce positive physiological responses when introduced into the body, making them useful ingredients in food, cosmetic and pharmaceutical products. However, the full potential of BPs has not been fully explored because research is still lacking on economical and scalable production methods. This study is therefore aimed at the development of a bioprocess for the production of novel bioactive peptides from food proteins by exploiting fermentative and the proteolytic activities of Lactobacillus delbrueckii subsp. lactis 313 (LDL 313). The optimum parameters and conditions for the production of antihypertensive peptides using LDL 313 were obtained from the literature and covered the upstream, midstream and downstream stages. The feasibility of manufacturing BPs in a large scale was also projected by conducting an economic assessment and a quantitative analysis based on the medical needs of hypertensive patients in Malaysia. Results indicated that to meet the needs of the hypertensive population, a production level of 1.267 kg milk protein/kg peptide had to be obtained at an enzyme requirement of 0.2 kg enzyme/kg milk protein. At this production level and at a peptide market value of USD 88.5/g peptide, the annual expected value of peptide will be USD 41,858,307,460. It will take about 19.5 h to produce one batch of the peptides at a total annual cost (equipment and utilities) of USD 15,081,885. An annual revenue of USD 42 billion is therefore expected from the entire bioprocess implying that the process is economically feasible.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Agyei D, Danquah MK (2011) Industrial-scale manufacturing of pharmaceutical-grade bioactive peptides. Biotechnol Adv 29(3):272–277. doi:10.1016/j.biotechadv.2011.01.001
Agyei D, Danquah MK (2012a) Rethinking food-derived bioactive peptides for antimicrobial and immunomodulatory activities. Trends Food Sci Technol 23(2012):62–69. doi:10.1016/j.tifs.2011.08.010
Agyei D, Danquah MK (2012b) Carbohydrate utilization affects Lactobacillus delbrueckii subsp. lactis 313 cell-enveloped-associated proteinase production. Biotechnol Bioprocess Eng 17(4):787–794. doi:10.1007/s12257-012-0106-2
Agyei D, Danquah MK (2012c) In-depth characterisation of Lactobacillus delbrueckii subsp. lactis 313 for growth and cell-envelope-associated proteinase production. Biochem Eng J 64:61–68. doi:10.1016/j.bej.2012.03.006
Agyei D, Potumarthi R, Danquah MK (2012) Optimisation of batch culture conditions for cell-envelope-associated proteinase production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™. Appl Biochem Biotechnol 168:1035–1050. doi:10.1007/s12010-012-9839-9
Agyei D, Lim W, Zass M, Tan D, Danquah MK (2013a) Bioanalytical Evaluation of Lactobacillus delbrueckii subsp. lactis 313 Cell-envelope proteinase extraction. Chem Eng Sci 95(2013):323–330. doi:10.1016/j.ces.2013.03.049
Agyei D, Potumarthi R, Danquah, MK (2013b) Production of lactobacilli proteinases for the manufacture of bioactive peptides: part i—upstream processes. In Kim SK (ed) Marine proteins and peptides. Wiley, New York, pp 207–229
Agyei D, Potumarthi R, Danquah MK (2013c) Production of lactobacilli proteinases for the manufacture of bioactive peptides: part ii—downstream processes. Mar Proteins Peptides. Wiley, New York, pp 231–251
Agyei D, Danquah MK, Sarethy IP, Pan S (2015). Antioxidative peptides derived from food proteins. In: Rani V, Yadav UCS (eds) Free radicals in human health and disease. Springer, India, pp 417–430
Aluko R (2008) Bioactive peptides. SciTopics 2012, from http://www.scitopics.com/Bioactive_Peptides.html
Axelsson L (2004) Lactic acid bacteria: classification and physiology. In: Salminen S, von Wright A, Ouwehand A (eds) Lactic acid bacteria. Microbiological and functional aspects, 3rd edn. Marcel Dekker, New York, pp 1–66
Cicerale S, Conlan XA, Barnett NW, Keast RS (2011) The concentration of oleocanthal in olive oil waste. Nat Prod Res 25(5):542–548. doi:10.1080/14786419.2010.511214
Celenza G (2000) Industrial waste treatment processes engineering: specialized treatment systems, vol 3. CRC Press.
Clare DA, Swaisgood HE (2000) Bioactive milk peptides: a prospectus. J Dairy Sci 83(6):1187–1195
Espeche Turbay MB, Savoy de Giori G, Hebert EM (2009) Release of the cell-envelope-associated proteinase of Lactobacillus delbrueckii subspecies lactis CRL 581 is dependent upon pH and temperature. J Agric Food Chem 57(18):8607–8611. doi:10.1021/jf901531q
Gul K, Singh AK, Jabeen R (2015) Nutraceuticals and functional foods: the foods for future world. Crit Rev Food Sci Nutr. doi:10.1080/10408398.2014.903384
Guo Y, Pan D, Tanokura M (2009) Optimisation of hydrolysis conditions for the production of the angiotensin-I converting enzyme (ACE) inhibitory peptides from whey protein using response surface methodology. Food Chem 114(1):328–333. doi:http://dx.doi.org/10.1016/j.foodchem.2008.09.041
Gupta R, Beg QK, Khan S, Chauhan B (2002) An overview on fermentation, downstream processing and properties of microbial alkaline proteases. Appl Microbiol Biotechnol 60(4):381–395. doi:10.1007/s00253-002-1142-1
Hancock REW, Sahl H-G (2006) Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat Biotechnol 24(12):1551–1557. doi:10.1038/nbt1267
Hettiarachchy NS, Kalapathy U (1998) Functional properties of soy proteins. In: Functional properties of proteins and lipids, vol 708. American Chemical Society, New York, pp 80–95
Hughes GJ, Ryan DJ, Mukherjea R, Schasteen CS (2011) Protein digestibility-corrected amino acid scores (PDCAAS) for soy protein isolates and concentrate: criteria for evaluation. J Agric Food Chem 59(23):12707–12712. doi:10.1021/jf203220v
Kalogeropoulos N, Tsimidou M (2014) Antioxidants in Greek Virgin Olive Oils. Antioxidants 3(2):387–413
Kaushik JK, Kumar A, D RK, Mohanty AK, Grover S, Batish VK, Sechi LA (2009) Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum. PLoS ONE 4(12):e8099. doi:10.1371/journal.pone.0008099
Khalid NM, Marth EH (1990) Lactobacilli—their enzymes and role in ripening and spoilage of cheese: a review. J Dairy Sci 73(10):2669–2684. doi:10.3168/jds.S0022-0302(90)78952-7
Korhonen H (2009) Milk-derived bioactive peptides: From science to applications. J Funct Foods 1(2):177–187. doi:10.1016/j.jff.2009.01.007
Korhonen H, Pihlanto A (2003) Food-derived bioactive peptides—opportunities for designing future foods. Curr Pharm Des 9(16):1297–1308. doi:10.2174/1381612033454892
Korhonen H, Pihlanto A (2006) Bioactive peptides: production and functionality. Int Dairy J 16(9):945–960. doi:10.1016/j.idairyj.2005.10.012
Korhonen HJ, Marnila P (2013) Milk bioactive proteins and peptides. In: Milk and dairy products in human nutrition. Wiley, New York, pp 148–171
Mahugo Santana C, Sosa Ferrera Z, Esther Torres Padron M, Juan Santana Rodriguez J (2009) Methodologies for the extraction of phenolic compounds from environmental samples: new approaches. Molecules 14(1):298–320. doi:10.3390/molecules14010298
Mancebo-Campos V, Salvador MD, Fregapane G (2014) Antioxidant capacity of individual and combined virgin olive oil minor compounds evaluated at mild temperature (25 and 40 °C) as compared to accelerated and antiradical assays. Food Chem 150:374–381. doi:10.1016/j.foodchem.2013.10.162
Ozan Nazim C, Deniz C, Ehsan J (2012) Potential applications of green technologies in olive oil industry. In: Boskou D (ed) Olive oil—constituents, quality, health properties and bioconversions
Park SY, Lee J-S, Baek H-H, Lee HG (2010) Purification and characterization of antioxidant peptides from soy protein hydrolysate. J Food Biochem 34:120–132. doi:10.1111/j.1745-4514.2009.00313.x
Pihlanto-Leppälä A (2002) MILK PROTEINS|Bioactive peptides. In: Hubert R (ed) Encyclopedia of dairy sciences. Elsevier, Oxford, pp 1960–1967
Reboredo-Rodríguez P, Rey-Salgueiro L, Regueiro J, González-Barreiro C, Cancho-Grande B, Simal-Gándara J (2014) Ultrasound-assisted emulsification–microextraction for the determination of phenolic compounds in olive oils. Food Chem 150:128–136. doi:10.1016/j.foodchem.2013.10.157
Ren X, Pan D, Wu Z, Zeng X, Sun Y, Cao J, Guo Y (2014) Limited hydrolysis of β-casein by cell wall proteinase and its effect on hydrolysates’s conformational and structural properties. Int J Food Sci Technol. doi:10.1111/ijfs.12705
Shurtleff W, Aoyagi A (2010) History of soybeans and soyfoods in Southeast Asia (13th century to 2010): extensively annotated bibliography and sourcebook. Soyinfo Center
Singh P, Kumar R, Sabapathy SN, Bawa AS (2008) Functional and edible uses of soy protein products. Compr Rev Food Sci Food Safety 7(1):14–28. doi:10.1111/j.1541-4337.2007.00025.x
Vertuani S, Beghelli E, Scalambra E, Malisardi G, Copetti S, Dal Toso R, Manfredini S (2011) Activity and stability studies of verbascoside, a novel antioxidant, in dermo-cosmetic and pharmaceutical topical formulations. Molecules 16(8):7068–7080
Zacharof M-P, Coss GM, Mandale SJ, Lovitt RW (2013) Separation of lactobacilli bacteriocins from fermented broths using membranes. Process Biochem 48(8):1252–1261. doi:http://dx.doi.org/10.1016/j.procbio.2013.05.017
Zaks A, Klibanov AM (1985) Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci 82(10):3192–3196
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this chapter
Cite this chapter
Gnasegaran, G.K., Agyei, D., Pan, S., Sarethy, I.P., Acquah, C., Danquah, M.K. (2017). Process Development for Bioactive Peptide Production. In: Puri, M. (eds) Food Bioactives. Springer, Cham. https://doi.org/10.1007/978-3-319-51639-4_4
Download citation
DOI: https://doi.org/10.1007/978-3-319-51639-4_4
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-51637-0
Online ISBN: 978-3-319-51639-4
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)