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Analysis of Epithelial Injury and Repair

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Acute Lung Injury and Repair

Part of the book series: Respiratory Medicine ((RM))

Abstract

Alveolar epithelial injury is a feature of ARDS. In this chapter, we will address strategies to analyze alveolar epithelial cell function in lung injury and repair processes. These include an overview of cell isolation procedures for murine and human alveolar epithelial type II cells and examples for phenotypic characterization of ATII cells in the context of lung injury. Moreover, we will discuss functional in vitro assays to evaluate differentiation and regeneration potential of alveolar epithelial cells.

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Abbreviations

α-SMA:

Alpha smooth muscle actin

ALI:

Acute lung injury

ABCA3:

ATP-binding cassette sub-family A member 3

AQP5:

Aquaporin 5

ATI:

Alveolar epithelial type I cell

ATII:

Alveolar epithelial type II cell

BMP:

Bone morphogenetic protein

BrdU:

Bromodeoxyuridine

COPD:

Chronic obstructive pulmonary disease

ECAD:

E-cadherin, epithelial cadherin

ECM:

Extracellular matrix

EGFP:

Enhanced green fluorescent protein

EMT:

Epithelial-to-mesenchymal transition

EpCAM:

Epithelial cell adhesion molecule

GFP:

Green fluorescent protein

FACS:

Fluorescence Activated Cell Sorting

IPF:

Idiopathic pulmonary fibrosis

KGF:

Keratinocyte growth factor

LAMP3:

Lysosome-associated membrane glycoprotein 3

PARP:

Poly (ADP-ribose) polymerase

RAGE:

Receptor for advanced glycosylation end products

SPA:

Surfactant protein A

SPB:

Surfactant protein B

SPC:

Surfactant protein C

SPD:

Surfactant protein D

TGF-β:

Transforming growth factor beta

T1α:

Podoplanin

TJ:

Tight junctions

TJP1:

Tight junction protein 1

TUNEL:

TdT-mediated dUTP-biotin nick end labeling

WNT:

Wingless-type MMTV integration site family member

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Authors and Affiliations

  1. Comprehensive Pneumology Center (CPC), Helmholtz Zentrum Munich and Ludwig-Maximilians-University Munich, Max-Lebsche-Platz 31, 81377, Munich, Germany

    Kathrin Mutze & Melanie Königshoff

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  1. Kathrin Mutze
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  2. Melanie Königshoff
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Correspondence to Melanie Königshoff .

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  1. Pulmonary, Critical Care, Allergy and Sleep Medicine, Medical University of South Carolina, Charleston, South Carolina, USA

    Lynn M. Schnapp

  2. Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA

    Carol Feghali-Bostwick

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Mutze, K., Königshoff, M. (2017). Analysis of Epithelial Injury and Repair. In: Schnapp, L., Feghali-Bostwick, C. (eds) Acute Lung Injury and Repair. Respiratory Medicine. Humana Press, Cham. https://doi.org/10.1007/978-3-319-46527-2_6

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