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Biobanking of Human Mesenchymal Stem Cells: Future Strategy to Facilitate Clinical Applications

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Biobanking and Cryopreservation of Stem Cells

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 951))

Abstract

Human mesenchymal stem cells (hMSCs), a type of adult stem cells that hold great potential in clinical applications (e.g., regenerative medicine and cell-based therapy) due to their ability to differentiate into multiple types of specialized cells and secrete soluble factors which can initiate tissue repair and regulate immune response. hMSCs need to be expanded in vitro or cryopreserved to obtain sufficient cell numbers required for clinical applications. However, long-term in vitro culture-expanded hMSCs may raise some biosafety concerns (e.g., chromosomal abnormality and malignant transformation) and compromised functional properties, limiting their use in clinical applications. To avoid those adverse effects, it is essential to cryopreserve hMSCs at early passage and pool them for off-the-shelf use in clinical applications. However, the existing cryopreservation methods for hMSCs have some notable limitations. To address these limitations, several approaches have to be taken in order to produce healthy and efficacious cryopreserved hMSCs for clinical trials, which remains challenging to date. Therefore, a noteworthy amount of resources has been utilized in research in optimization of the cryopreservation methods, development of freezing devices, and formulation of cryopreservation media to ensure that hMSCs maintain their therapeutic characteristics without raising biosafety concerns following cryopreservation. Biobanking of hMSCs would be a crucial strategy to facilitate clinical applications in the future.

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Abbreviations

hMSCs:

Human mesenchymal stem cells

CPA:

Cryoprotective agent

DMSO:

Dimethyl sulfoxide

FBS:

Fetal bovine serum

GvHD:

Graft-versus-host disease

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Acknowledgements

This report was supported by University of Malaya Research Grant (UMRG RP040B-15HTM) from the Ministry of Education Malaysia.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia

    Kar Wey Yong, Jane Ru Choi & Wan Kamarul Zaman Wan Safwani

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  1. Kar Wey Yong
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  2. Jane Ru Choi
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  3. Wan Kamarul Zaman Wan Safwani
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Correspondence to Wan Kamarul Zaman Wan Safwani .

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Editors and Affiliations

  1. Department of Oncology, University of Alberta, Edmonton, Alberta, Canada

    Feridoun Karimi-Busheri

  2. Department of Oncology, Cross Cancer Institute and University of Alberta, Edmonton, Alberta, Canada

    Michael Weinfeld

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Yong, K.W., Choi, J.R., Wan Safwani, W.K.Z. (2016). Biobanking of Human Mesenchymal Stem Cells: Future Strategy to Facilitate Clinical Applications. In: Karimi-Busheri, F., Weinfeld, M. (eds) Biobanking and Cryopreservation of Stem Cells. Advances in Experimental Medicine and Biology, vol 951. Springer, Cham. https://doi.org/10.1007/978-3-319-45457-3_8

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