Abstract
Enzyme analysis is used for many purposes in food science and technology, including to indicate adequate processing, to assess enzyme preparations, and to measure constituents of foods that are enzyme substrates. In this laboratory exercise, the glucose content of corn syrup solids is determined in a coupled reaction using the enzymes glucose oxidase and peroxidase. Glucose oxidase catalyzes the oxidation of glucose to form hydrogen peroxide, which then reacts with a dye in the presence of peroxidase to give a stable-colored product that can be quantitated spectrophotometrically.
Resource Materials
BeMiller JN (2017) Carbohydrate analysis. Ch. 19. In: Nielsen SS (ed) Food analysis, 5th edn. Springer, New York
Reyes-De-Coreuera JI, and Powers JR (2017) Application of enzymes in food analysis. Ch. 26. In: Nielsen SS (ed) Food analysis, 5rd edn. Springer, New York
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Carpenter, C.E., Ward, R.E. (2017). Glucose Determination by Enzyme Analysis. In: Food Analysis Laboratory Manual. Food Science Text Series. Springer, Cham. https://doi.org/10.1007/978-3-319-44127-6_24
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DOI: https://doi.org/10.1007/978-3-319-44127-6_24
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Online ISBN: 978-3-319-44127-6
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