Skip to main content
SpringerLink
Log in

Wool Proteomics

  • Chapter
  • First Online:
Agricultural Proteomics Volume 1

Abstract

The recent sequencing of the wool genome has considerably assisted the characterisation of the proteome of wool fibres. This has been achieved by the coupling of mass spectral sequence identification to the traditional two-dimensional gel electrophoresis, resulting in the identification of most of the protein spots on the two-dimensional map. More recently, gel-free proteomic analysis of whole wool protein digests has resulted in improved sequence coverage of some proteins and the identification of novel isoforms of some of these proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Abbreviations

KAP:

Keratin Associated Protein

HSP:

High Sulphur Protein

UHSP:

Ultra-High Sulphur Protein

HGTP:

High Glycine-Tyrosine Protein

2DE:

Two-Dimensional Electrophoresis

MALDI:

Matrix Assisted Adsorption/Desorption Ionisation

TOF:

Time of Flight

MS:

Mass Spectrometry

LC:

Liquid Chromatography

ESI:

Electro-Spin Ionisation

References

  1. Shorland FB, Gray JM (1970) The preparation of nutritious protein from wool. Br J Nutr 24:717

    Article  CAS  PubMed  Google Scholar 

  2. Gillespie JM, Goldsmith LA (1983) The structural proteins of hair: Isolation, characterization, and regulation of biosynthesis. In: Biochemistry and physiology of the skin. Oxford University Press, New York, pp 475–510

    Google Scholar 

  3. Maclaren JA, Milligan B (1981) Wool science—the chemical reactivity of the wool fibre. Science Press, Marrickville

    Google Scholar 

  4. Parry DAD, Steinert PM (1995) Intermediate filament structure. Springer, Heidelberg

    Google Scholar 

  5. Marshall RC, Orwin DFG, Gillespie JM (1991) Structure and biochemistry of mammalian hard keratin. Electron Microscope Rev 4:47–83

    Article  CAS  Google Scholar 

  6. Powell BC (1996) The keratin proteins and genes of wool and hair. Wool Technol Sheep Breed 44:100–118

    Google Scholar 

  7. Marshall RC (1981) Analysis of the proteins from single wool fibers by two-dimensional polyacrylamide gel electrophoresis. Text Res J 51:106–108

    Article  CAS  Google Scholar 

  8. Marshall RC, Blagrove RJ (1979) Successful isoelectric focusing of wool low-sulphur proteins. J Chromatogr A 172:351–356

    Article  CAS  Google Scholar 

  9. Herbert BR, Woods JL (1994) Immobilised pH gradient isoelectric focusing of wool proteins. Electrophoresis 15:972–976

    Article  CAS  PubMed  Google Scholar 

  10. Clerens S, Cornellison CD, Deb-Choudhury S, Thomas A, Plowman JE, Dyer JM (2010) Developing the wool proteome. J Proteomics 73:1722–1731

    Google Scholar 

  11. Crewther WG, Dowling LM, Gough KH, Inglis AS, Mckern NM, Sparrow LG et al (1975) The low-sulphur proteins of wool: studies on their classification, characterization, primary and secondary structure. In: Proceedings of the 5th international wool textile research conference. Aachen, Germany, pp 233–242

    Google Scholar 

  12. Crewther WG, Dowling LM, Gough KH, Marshall RC, Sparrow LG, Parry DAD et al (1980) The microfibrillar proteins of α-keratin. In: Fibrous proteins: scientific, industrial and medical aspects. Academic Press Inc. (London) Ltd., London, pp 151–159

    Google Scholar 

  13. Yu Z, Gordon SW, Nixon AJ, Bawden CS, Rogers MA, Wildermoth JE et al (2009) Expression patterns of keratin intermediate filament and keratin associated protein genes in wool follicles. Differentiation 77:307–316

    Article  PubMed  Google Scholar 

  14. Yu Z, Wildermoth JE, Wallace OAM, Gordon SW, Maqbool NJ, Maclean P et al (2011) Annotations of sheep keratin intermediate filament genes and their patterns of expression. Exp Dermatol 20:582–588

    Article  CAS  PubMed  Google Scholar 

  15. Rogers GR, Hickford JG, Bickerstaffe R (1994) Polymorphism in two genes for B2 high sulfur proteins of wool. Anim Genet 25:407–415

    Article  CAS  PubMed  Google Scholar 

  16. Itenge-Mweza TO, Forrest RJH, Mckenzie G, Abbott J, Amoafo O, Hickford JG (2007) Polymorphism of the KAP1.1, KAP1.3 and KRT.1.2 genes in merino sheep. Mol Cell Probes 21:338–342

    Article  CAS  PubMed  Google Scholar 

  17. Gong H, Zhou H, Plowman JE, Dyer JM, Hickford JGH (2010) Analysis of variation in the ovine ultra-high sulphur keratin-associated protein KAP5-4 gene using PCR-SSCP technique. Electrophoresis 31:3545–3547

    Article  CAS  PubMed  Google Scholar 

  18. Gong H, Zhou H, Plowman JE, Dyer JM, Hickford JG (2011) Search for variation in the ovine KAP7-1 and KAP8-1 genes using PCR-SSCP. DNA Cell Biol 31:367–370

    Article  PubMed  Google Scholar 

  19. Gong H, Zhou H, Hickford JG (2010) Polymorphism of the ovine keratin-associated-protein 1-4 (KRTAP1-4) gene. Mol Biol Rep 37:3377–3380

    Article  CAS  PubMed  Google Scholar 

  20. Gong H, Zhou H, Dyer J, Hickford J (2011) Identification of the ovine KAP11-1 gene (KRTAP11-1) and genetic variation in its coding sequence. Mol Biol Rep 38:5429–5433

    Article  CAS  PubMed  Google Scholar 

  21. Gong H, Zhou H, Hickford JG (2011) Diversity of the glycine/tyrosine-rich keratin-associated protein 6 gene (KAP6) family in sheep. Mol Biol Rep 38:31–35

    Article  CAS  PubMed  Google Scholar 

  22. Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM et al (2006) New consensus nomenclature for mammalian keratins. J Cell Biol 174:169–174

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Rogers MA, Langbein L, Winter H, Ehmann C, Praetzel S, Korn B et al (2001) Characterization of a cluster of human high/ultrahigh sulfur keratin-associated protein genes embedded in the type I keratin gene domain on chromosome 17q12-21. J Biol Chem 276:19440–19451

    Article  CAS  PubMed  Google Scholar 

  24. Gong H, Zhou H, Mckenzie GW, Yu Z, Clerens S, Dyer JM et al (2012) An updated nomenclature for keratin-associated proteins (KAPs). Int J Biol Sci 8:258–264

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Deb-Choudhury S, Plowman JE, Thomas A, Krsinic GL, Dyer JM, Clerens S (2010) Electrophoretic mapping of highly homologous keratins: a novel marker peptide approach. Electrophoresis 31:2894–2902

    Article  CAS  PubMed  Google Scholar 

  26. Plowman JE, Deb-Choudhury S, Thomas A, Clerens S, Cornellison CD, Grosvenor AJ et al (2010) Characterisation of low abundance wool proteins through novel differential extraction techniques. Electrophoresis 31:1937–1946

    Article  CAS  PubMed  Google Scholar 

  27. Woods JL, Orwin DFG (1987) Wool proteins of New Zealand romney sheep. Aust J Biol Sci 40:1–14

    CAS  PubMed  Google Scholar 

  28. Flanagan LM, Plowman JE, Bryson WG (2002) The high sulphur proteins of wool: towards an understanding of sheep breed diversity. Proteomics 2:1240–1246

    Article  CAS  PubMed  Google Scholar 

  29. Bringans SD, Plowman JE, Dyer JM, Clerens S, Vernon JA, Bryson WG (2007) Characterization of the exocuticle a-layer proteins of wool. Exp Dermatol 16:951–960

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Food & Bio-Based Products, AgResearch Lincoln Research Centre, Lincoln, New Zealand

    Jeffrey E. Plowman & Santanu Deb-Choudhury

Authors
  1. Jeffrey E. Plowman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Santanu Deb-Choudhury
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jeffrey E. Plowman .

Editor information

Editors and Affiliations

  1. Systems Biology, Agr. Biotech. Res. Institute of Iran, Karaj, Iran

    Ghasem Hosseini Salekdeh

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Plowman, J.E., Deb-Choudhury, S. (2016). Wool Proteomics. In: Salekdeh, G. (eds) Agricultural Proteomics Volume 1. Springer, Cham. https://doi.org/10.1007/978-3-319-43275-5_11

Download citation

Publish with us

Policies and ethics

Search

Navigation