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Sensor Systems with Magnetic and Optomagnetic Readout of Rolling Circle Amplification Products

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Rolling Circle Amplification (RCA)

Abstract

We are developing robust biosensors for homogeneous detection of rolling circle amplification (RCA) products with magnetic and/or optomagnetic readouts based on surface-functionalized magnetic nanoparticles. Binding of RCA amplicons to nanoparticles modifies their ability to rotate in response to an applied oscillating magnetic field. As a result, magnetic or optical measurements of these changes in the rotational response of nanoparticles vs. frequency of the magnetic field can be used to quantitate the number of amplicons, and, hence, the concentration of target nucleic acid analytes. After describing the basic principles of this approach, we present the current status of the development of compact and portable sensing devices used to measure the dynamic response of magnetic particle suspensions. Then, we give examples and results of different RCA detection strategies designed by us, and we also outline future directions for this innovative diagnostic approach.

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References

  • Akhtar S, Strömberg M, Zardán Gómez de la Torre T et al (2010) Real-space transmission electron: microscopy investigations of attachment of functionalized magnetic nanoparticles to DNA-coils acting as a biosensor. J Phys Chem B 114:13255–13262

    Article  CAS  PubMed  Google Scholar 

  • Banér J, Nilsson M, Mendel-Hartvig M et al (1998) Signal amplification of padlock probes by rolling circle replication. Nucleic Acids Res 26:5073–5078

    Article  PubMed  PubMed Central  Google Scholar 

  • Bejhed RS, Zardán Gómez de la Torre T, Donolato M et al (2015a) Turn-on optomagnetic bacterial DNA sequence detection using volume-amplified magnetic nanobeads. Biosens Bioelectron 66:405–411

    Article  CAS  PubMed  Google Scholar 

  • Dahl F, Banér J, Gullberg M et al (2004) Circle-to-circle amplification for precise and sensitive DNA analysis. Proc Natl Acad Sci U S A 101:4548–4553

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dalslet BT, Damsgaard CD, Donolato M et al (2011) Bead magnetorelaxometry with an on-chip magnetoresistive sensor. Lab Chip 11:296–302

    Article  CAS  PubMed  Google Scholar 

  • Debye P (1929) Polar molecules. Chemical Catalogue Company, New York

    Google Scholar 

  • Dieckhoff JH, Yoshida T, Enpuku K et al (2012) Homogeneous bioassays based on the manipulation of magnetic nanoparticles by rotating and alternating magnetic fields—a comparison. IEEE Trans Magn 48:3792–3795

    Article  CAS  Google Scholar 

  • Donolato M, Antunes P, Bejhed RS et al (2015a) Novel readout method for molecular diagnostic assays based on optical measurements of magnetic nanobead dynamics. Anal Chem 87:1622–1629

    Article  CAS  PubMed  Google Scholar 

  • Donolato M, Antunes P, Zardán Gómez de la Torre T et al (2015b) Quantification of rolling circle amplified DNA using magnetic nanobeads and a Blu-ray optical pick-up unit. Biosens Bioelectron 67:649–655

    Article  CAS  PubMed  Google Scholar 

  • Engström A, Zardán Gómez de la Torre T, Strømme M et al (2013) Detection of Rifampicin resistance in Mycobacterium tuberculosis by padlock probes and magnetic nanobead-based readout. PLoS One 8:e62015

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Göransson J, Zardán Gómez de la Torre T, Strömberg M et al (2010) Sensitive detection of bacterial DNA by magnetic nanoparticles. Anal Chem 82:9138–9140

    Article  CAS  PubMed  Google Scholar 

  • Jarvius J, Melin J, Göransson J et al (2006) Digital quantification using amplified single molecule detection. Nat Methods 3:725–727

    Article  CAS  PubMed  Google Scholar 

  • Mezger A, Fock J, Antunes P et al (2015) Scalable DNA-based magnetic nanoparticle agglutination assay for bacterial detection in patient samples. ACS Nano 9:7374–7382

    Article  CAS  PubMed  Google Scholar 

  • Nilsson M, Malmgren H, Samiotaki M et al (1994) Padlock probes: circularizing oligonucleotides for localized DNA detection. Science 265:2085–2088

    Article  CAS  PubMed  Google Scholar 

  • Østerberg FW, Rizzi G, Hansen MF (2012) On-chip measurements of Brownian relaxation vs. concentration of 40 nm magnetic beads. J Appl Phys 12:124512

    Google Scholar 

  • Østerberg FW, Rizzi G, Zardán Gómez de la Torre T et al (2013a) Measurements of Brownian relaxation of magnetic nanobeads using planar Hall effect bridge sensors. Biosens Bioelectron 40:147–152

    Article  CAS  PubMed  Google Scholar 

  • Østerberg FW, Rizzi G, Hansen MF (2013b) On-chip measurements of Brownian relaxation of magnetic beads with diameters from 10 nm to 250 nm. J Appl Phys 113:154507

    Article  CAS  Google Scholar 

  • Østerberg FW, Rizzi G, Donolato M et al (2014) On-chip detection of rolling circle amplified DNA molecules from Bacillus globigii spores and Vibrio cholerae. Small 10:2877–2882

    Article  CAS  PubMed  Google Scholar 

  • Smolina IV, Cherny DI, Nietupski RM et al (2005) High-density fluorescently labeled rolling-circle amplicons for DNA diagnostics. Anal Biochem 347(1):152–155

    Article  CAS  PubMed  Google Scholar 

  • Strömberg M, Göransson J, Gunnarsson K et al (2008a) Sensitive molecular diagnostics using volume-amplified magnetic nanobeads. Nano Lett 8:816–821

    Article  CAS  PubMed  Google Scholar 

  • Strömberg M, Zardán Gómez de la Torre T, Göransson J et al (2008b) Microscopic mechanisms influencing the volume amplified magnetic nanobead detection assay. Biosens Bioelectron 24:696–703

    Article  CAS  PubMed  Google Scholar 

  • Strömberg M, Zardán Gómez de la Torre T, Göransson J et al (2009) Multiplex detection of DNA sequences using the volume-amplified magnetic nanobead detection assay. Anal Chem 81:3398–3406

    Article  CAS  PubMed  Google Scholar 

  • Strömberg M, Akhtar S, Gunnarsson K et al (2011) Immobilization of oligonucleotide-functionalized magnetic nanobeads in DNA-coils studied by electron microscopy and atomic force microscopy. MRS Online Proc Libr 1:1355

    Google Scholar 

  • Strömberg M, Zardán Gómez de la Torre T, Nilsson M et al (2014) A magnetic nanobead-based bioassay provides sensitive detection of single- and biplex bacterial DNA using a portable AC susceptometer. Biotechnol J 9:137–145

    Article  CAS  PubMed  Google Scholar 

  • Yang J, Donolato M, Pinto A et al (2016) Blu-ray based optomagnetic aptasensor for detection of small molecules. Biosens Bioelectron 75:396–403

    Article  CAS  PubMed  Google Scholar 

  • Zardán Gómez de la Torre T, Strömberg M, Russel C et al (2010) Investigation of immobilization of functionalized magnetic nanobeads in rolling circle amplified DNA coils. J Phys Chem B 114:3707–3713

    Article  CAS  PubMed  Google Scholar 

  • Zardán Gómez de la Torre T, Mezger A, Herthnek D et al (2011a) Detection of rolling circle amplified DNA molecules using probe-tagged magnetic nanobeads in a portable AC susceptometer. Biosens Bioelectron 29:195–199

    Article  CAS  Google Scholar 

  • Zardán Gómez de la Torre T, Ke R, Mezger A et al (2012) Sensitive detection of spores using volume-amplified magnetic nanobeads. Small 8:2174–2177

    Article  CAS  Google Scholar 

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Acknowledgments

Financial support from EU FP7 Grant No. 604448-NanoMag, SSF grant SBE13-0125 Fluid, and u-forsk VR grant 2015-03640 is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345B, Kongens Lyngby, 2800, Denmark

    Mikkel F. Hansen Ph.D., Marco Donolato Ph.D. & Jeppe Fock Ph.D.

  2. BluSense Diagnostics, Symbion Bioscience Park, Fruebjergvej 3, Box 68, Copenhagen Ø, 2100, Denmark

    Marco Donolato Ph.D.

  3. Division of Solid State Physics, Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, Uppsala, SE-751 21, Sweden

    Mattias Strömberg Ph.D. & Peter Svedlindh Ph.D.

  4. Division of Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala University, The Ångström Laboratory, Box 534, Uppsala, SE-751 21, Sweden

    Maria Strømme Ph.D.

Authors
  1. Mikkel F. Hansen Ph.D.
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  2. Marco Donolato Ph.D.
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  3. Jeppe Fock Ph.D.
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  4. Mattias Strömberg Ph.D.
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  5. Maria Strømme Ph.D.
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  6. Peter Svedlindh Ph.D.
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Corresponding author

Correspondence to Mikkel F. Hansen Ph.D. .

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Editors and Affiliations

  1. Global Prior Art Inc., Boston, Massachusetts, USA

    Vadim V. Demidov

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Hansen, M.F., Donolato, M., Fock, J., Strömberg, M., Strømme, M., Svedlindh, P. (2016). Sensor Systems with Magnetic and Optomagnetic Readout of Rolling Circle Amplification Products. In: Demidov, V. (eds) Rolling Circle Amplification (RCA). Springer, Cham. https://doi.org/10.1007/978-3-319-42226-8_11

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