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Physiochemical Methods for Detection of Occupational Contact Allergens

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Kanerva’s Occupational Dermatology

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Abstract

To diagnose and prevent allergic contact dermatitis the demonstration of allergens in products from the patient’s environment is important. Chemical analysis of a product can make it possible to demonstrate the presence or absence of known allergens.

There are simple spot tests that can be used to qualitatively demonstrate the presence of some of the most common contact allergens such as nickel ions, hexavalent chromium ions, cobalt ions, and formaldehyde.

For most substances more advanced chemical methods are needed. Such methods often consist of a chromatographic separation system and a detection system. Examples of separation techniques are thin-layer chromatography, high-performance liquid chromatography, and gas chromatography, while detection and quantification of substances can be done using e.g., UV-VIS spectrophotometry, mass spectrometry, and atomic absorption spectrophotometry.

Methods such as mass spectrometry, nuclear magnetic resonance spectroscopy, and infrared spectrophotometry are often required to identify isolated allergens.

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Authors and Affiliations

  1. Department of Occupational and Environmental Dermatology, Lund University, Skåne University Hospital (SUS), Malmö, Sweden

    Malin Engfeldt, Birgitta Gruvberger & Magnus Bruze

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  1. Malin Engfeldt
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  2. Birgitta Gruvberger
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  3. Magnus Bruze
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Correspondence to Magnus Bruze .

Editor information

Editors and Affiliations

  1. Department of Dermatology, University of Osnabrück, Osnabrück, Niedersachsen, Germany

    Swen Malte John

  2. Department of Dermato-allergology, Gentofte Hospital, Hellerup, Denmark

    Jeanne Duus Johansen

  3. Dept. of Dermatology and Allergology, VU University Medical Centre, Amsterdam, The Netherlands

    Thomas Rustemeyer

  4. Dept. of Dermatology and Allergology, Friedrich Schiller University, Jena, Thüringen, Germany

    Peter Elsner

  5. Department of Dermatology, University of California San Francisco, San Francisco, California, USA

    Howard I. Maibach

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Engfeldt, M., Gruvberger, B., Bruze, M. (2018). Physiochemical Methods for Detection of Occupational Contact Allergens. In: John, S., Johansen, J., Rustemeyer, T., Elsner, P., Maibach, H. (eds) Kanerva’s Occupational Dermatology. Springer, Cham. https://doi.org/10.1007/978-3-319-40221-5_86-2

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  • DOI: https://doi.org/10.1007/978-3-319-40221-5_86-2

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-40221-5

  • Online ISBN: 978-3-319-40221-5

  • eBook Packages: Springer Reference MedicineReference Module Medicine

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