Abstract
Organ failure and the demand for donor organs for transplantation has resulted in recent progress within the field of tissue engineering and increased functionality in the development of tissues and whole organs. This complex field brings together many different techniques and technologies in an attempt to build a tissue construct that can support cell growth and fully integrate into the body after transplantation. Electroporation is one technology that has been applied towards various applications within the field of tissue engineering. For example, nonthermal irreversible electroporation (NTIRE) has been shown as a potential method for tissue decellularization, producing tissue- and organ-derived scaffolds by removing the cellular content while preserving important structural and biochemical features to support cell growth. This method has been applied both in vivo and ex vivo with the use of active perfusion. In addition, nonthermal irreversible electroporation (NTIRE) has been applied to develop natural scaffolds with controlled porosity to enable the development of vasculature within the newly developing tissue, allowing for nutrients to be delivered throughout. Reversible electroporation (RE) has also shown potential for applications within the field of tissue engineering. By creating temporary pores within the cell membrane, reversible electroporation can be used for gene transfection, resulting in transfected cells that express growth factors needed for vasculature development and also having applications for stem cell differentiation. With further research, these areas of electroporation may develop into impactful technologies for the field of tissue engineering.
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References
Baah-Dwomoh A, Rolong A, Gatenholm P, Davalos R (2015) The feasibility of using irreversible electroporation to introduce pores in bacterial cellulose scaffolds for tissue engineering. Appl Microbiol Biotechnol 99:4785–4794. doi:10.1007/s00253-015-6445-0
Badylak S (2004) Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. Transpl Immunol 12:367–377
Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32:3233–3243
Gilbert T, Sellaro T, Badylak S (2006) Decellularization of tissues and organs. Biomaterials 27:1083–1087
Jabbarzadeh E, Starnes T, Khan Y, Jlang T, Wirtel A, Deng M, Lv Q, Nair L, Doty S, Laurencin C (2008) Induction of angiogenesis in tissue-engineered scaffolds designed for bone repair: a combined gene therapy-cell transplantation approach. Proc Natl Acad Sci U S A 105(32):11099–11104
Jourabchi N, Beroukhim K, Tafti BA, Kee ST, Lee EW (2014) Irreversible electroporation (NanoKnife) in cancer treatment. Gastrointest Interv 3:8–18
Khan AA, Vishwakarma SK, Bardia A, Venkateshwarulu J (2014) Repopulation of decellularized whole organ scaffold using stem cells: an emerging technology for the development of neo-organ. J Artif Organs 17:291–300. doi:10.1007/s10047-014-0780-2
Lutolf MP, Hubbell JA (2005) Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nat Biotechnol 23(1):47–55
Maor E, Ivorra A, Leor J, Rubinsky B (2007) The effects of irreversible electroporation on blood vessels. Technol Cancer Res Treat 6:307–312
Maor E, Ivorra A, Rubinsky B (2009) Non thermal irreversible electroporation: novel technology for vascular smooth muscle cell ablation. PLoS One 4(3):e4757
Mellott A, Forrest M, Detamor M (2013) Physical non-viral gene delivery methods for tissue engineering. Ann Biomed Eng 41(3):446–468
Miyamoto S, Katz B, Lafrenie R, Yamada K (1998) Fibronectin and integrins in cell adhesion, signaling and morphogenesis. Ann N Y Acad Sci 857:119–129
Patnaik SS, Wang B, Weed B, Wertheim JA, Liao J (2014) Chapter 3 Decellularized scaffolds: concepts, methodologies, and applications in cardiac tissue engineering and whole-organ regeneration. In: From tissue regeneration: where nano-structure meets biology. Singapore: World Scientific
Phillips M, Maor E, Rubinsky B (2010) Non-thermal irreversible electroporation for tissue decellularization. J Biomech Eng 132(9):091003.1–091003.8
Phillips M, Maor E, Rubinsky B (2011) Principles of tissue engineering with non-thermal irreversible electroporation. J Heat Transf 133(1):011004.1–011004.8
Phillips M, Raju N, Padath T, Rubinsky B (2012) Irreversible electroporation on the small intestine. Br J Cancer 106(3):490–495
Rana D, Zreiqat H, Benkirane-Jessel N, Ramakrishna S, Ramalingam M (2015) Development of decellularized scaffolds for stem cell-driven tissue engineering. J Tissue Eng Regen Med. doi:10.1002/term.2061
Ren X, Ott HC (2014) On the road to bioartificial organs. Pflugers Arch 466(10):1847–1857. doi:10.1007/s00424-014-1504-4
Ruoslahti R (1989) Proteoglycans in cell regulation. J Biol Chem 264(23):13369–13372
Sano MB, Neal R, Garcia PA, Gerber D, Robertson J, Davalos R (2010a) Towards the creation of decellularized organ constructs using irreversible electroporation and active mechanical perfusion. Biomed Eng Online 9(83):1–16
Sano MB, Rojas AD, Gatenholm P, Davalos R (2010b) Electromagnetically controlled biological assembly of aligned bacterial cellulose nanofibers. Ann Biomed Eng 38(8):2475–2484. doi:10.1007/s10439-010-9999-0
Sarker M, Chen XB, Schreyer DJ (2015) Experimental approaches to vascularization within tissue engineering constructs. J Biomater Sci Polym Ed 26(12):683–734. doi:10.10880/09205063.2015.1059018
Sulaeva I, Henniges U, Rosenau T, Potthast A (2015) Bacterial cellulose as a material for wound treatment: properties and modifications. A review. Biotechnol Adv 33:1547, 25 pages
Van der Rest M, Garrone R (1991) Collagen family of proteins. FASEB J 5:2814–2823
Yang F, Cho SW, Son SM, Bogatyrev SR, Singh D, Green JJ, Mei Y, Park S, Bhang SH, Kim BS, Langer R, Anderson D (2010) Genetic engineering of human stem cells for enhanced angiogenesis using biodegradable polymeric nanoparticles. Proc Natl Acad Sci U S A 107(8):3317–3322
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Ho, M.P. (2017). Tissue Engineering with Electroporation. In: Miklavčič, D. (eds) Handbook of Electroporation. Springer, Cham. https://doi.org/10.1007/978-3-319-32886-7_49
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DOI: https://doi.org/10.1007/978-3-319-32886-7_49
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