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AFM Investigation of the Influence of Red Light Irradiation on Collagen

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XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016

Part of the book series: IFMBE Proceedings ((IFMBE,volume 57))

Abstract

Low level red laser has a wide range of medical applications over the last decades. However its impact on collagen nanoscale features still remains unknown. Thus, thin collagen films were formed as extracellular matrix (ECM) models and irradiated using low level red laser. The results were recorded using Atomic Force Microscopy (AFM). The research was conducted in two stages. Firstly, collagen fibrils that were not fully formed (i.e. the highly reproducible distance of 67nm between gap and overlapping regions which is defined as D – Band periodicity did not occur in all the regions across the fibrils) were used as a model of damaged fibrils and irradiated for short time periods. The results indicated that red light led to the full creation of the D – Band periodicity. Moreover, a 25.2% reduction of the average width of the fibrils was recorded. In the second stage collagen fibrils with natural occurring characteristics were irradiated using a long period continuous irradiation procedure and the results indicated that red laser altered the dimensions of the fibrils (the reduction of the average width of the fibrils was 14% while the increase in the average height of the fibrils was 5%). More-over, the red laser altered the mechanical properties of the fibrils (the increase of the average Young’s modulus value within the same area on the fibril, post irradiation, was 24.5%). The observed alterations of structural and mechanical properties of collagen fibrils under the influence of low level red laser will provide new insights on the medical applications of red low power irradiation.

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Authors and Affiliations

  1. Biomedical Optics and Applied Biophysics Laboratory, School of Electrical and Computer Engineering, National Technical University of Athens, 9, Iroon Polytechniou, Athens, Greece

    Stylianos Vasileios Kontomaris, Dido Yova, Kyriaki Sambani & Andreas Stylianou

  2. Cancer Biophysics Laboratory, Department of Mechanical and Manufacturing Engineering, Faculty of Engineering, University of Cyprus, Nicosia, Cyprus

    Andreas Stylianou

Authors
  1. Stylianos Vasileios Kontomaris
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  2. Dido Yova
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  3. Kyriaki Sambani
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  4. Andreas Stylianou
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Corresponding author

Correspondence to Stylianos Vasileios Kontomaris .

Editor information

Editors and Affiliations

  1. Department of Computer Science and Engineering, Frederick University, Nicosia, Cyprus

    Efthyvoulos Kyriacou

  2. Biomedical Research Foundation, Nicosia, Cyprus

    Stelios Christofides

  3. Department of Computer Science, University of Cyprus, Nicosia, Cyprus

    Constantinos S. Pattichis

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© 2016 Springer International Publishing Switzerland

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Kontomaris, S.V., Yova, D., Sambani, K., Stylianou, A. (2016). AFM Investigation of the Influence of Red Light Irradiation on Collagen. In: Kyriacou, E., Christofides, S., Pattichis, C. (eds) XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016. IFMBE Proceedings, vol 57. Springer, Cham. https://doi.org/10.1007/978-3-319-32703-7_54

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  • DOI: https://doi.org/10.1007/978-3-319-32703-7_54

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-32701-3

  • Online ISBN: 978-3-319-32703-7

  • eBook Packages: EngineeringEngineering (R0)

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