Abstract
Vitamin D status is usually assessed by the measurement of the serum 25-hydroxyvitamin D (25(OH)D) concentration. Serum 25(OH)D can be measured by immunoassays, HPLC and mass spectrometry, the latter being the gold standard. A high variability between methods and laboratories exists, leading to problems when comparing vitamin D status between countries and diagnosing vitamin D deficiency or inadequacy. Quality control and standardization programs are decreasing the variation. The measurement of serum 25(OH)D is influenced by vitamin D binding protein (DBP), which is in its turn influenced by pregnancy and disease. Genetic variants of DBP influence the binding to 25(OH)D and thereby the concentration. The measurement of serum 25(OH)D can also be influenced by other metabolites such as 24,25-dihydroxyvitamin D and the 3-epimer of 25(OH)D. It has become possible to measure the free (unbound) serum 25(OH)D, but the clinical consequences still are uncertain.
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Lips, P., van Schoor, N.M., de Jongh, R.T. (2016). Assessment of Vitamin D Status. In: Weaver, C., Daly, R., Bischoff-Ferrari, H. (eds) Nutritional Influences on Bone Health. Springer, Cham. https://doi.org/10.1007/978-3-319-32417-3_17
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