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Vascular Proteomics

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Manual of Cardiovascular Proteomics

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Abstract

Atherothrombosis remains one of the main causes of morbidity and mortality in the western countries. The evolution from the initial steps to clinical events in atherothrombosis is a continuum of integrated and increasingly complex biological processes. To understand a process of such complexity, there is a need of developing new techniques, which could help to identify novel mediators of atherothrombosis that could become potential diagnostic, prognostic and/or therapeutic targets. In this chapter, we have described vascular samples including cells, tissues and blood (circulating cells, lipoproteins and extracellular vesicles) used in proteomic studies, as well as potential challenges and limitations. Following this, we made a succinct description of proteomics technologies (gel-based and MS-based approaches) applied to vascular samples and we included some examples to highlight what proteomics have added to previous techniques and/or concepts. On the whole, proteomics, in combination with other complementary approaches, is emerging as a very powerful tool that is expected to improve the diagnosis and treatment of patients at high risk of suffering a cardiovascular event.

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Authors and Affiliations

  1. Vascular Research Lab, FIIS-Fundacion Jimenez Diaz, Autonoma University of Madrid, Madrid, Spain

    Jose Luis Martin-Ventura PhD, Elena Burillo PhD, Roxana Martinez-Pinna PhD, Diego Martinez-Lopez & Luis Miguel Blanco-Colio PhD

  2. Cardiovascular Proteomic Lab and Proteomic Unit, Vascular Pathophysiology Area, Centro Nacional De Investigaciones Cardiovasculares, Madrid, Spain

    Emilio Camafeita PhD, Juan Antonio Lopez PhD & Jesus Vazquez PhD

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  1. Jose Luis Martin-Ventura PhD
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  2. Elena Burillo PhD
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  3. Roxana Martinez-Pinna PhD
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  4. Diego Martinez-Lopez
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  5. Luis Miguel Blanco-Colio PhD
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Correspondence to Jose Luis Martin-Ventura PhD .

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Editors and Affiliations

  1. Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Giulio Agnetti

  2. Dept. of Physiology & Biophysics, University Mississippi Medical Center, Jackson, Mississippi, USA

    Merry L. Lindsey

  3. Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    D. Brian Foster

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Martin-Ventura, J.L. et al. (2016). Vascular Proteomics. In: Agnetti, G., Lindsey, M., Foster, D. (eds) Manual of Cardiovascular Proteomics. Springer, Cham. https://doi.org/10.1007/978-3-319-31828-8_5

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