Abstract
The use of plants as efficient biopharmaceutical factories has significantly increased in the past two decades. This is mainly due to advancements in plant biotechnology which pave the way to high-yield production of biopharmaceuticals in plants, combined with efforts made to optimize yield through upstream, downstream, and preservation strategies of recombinant proteins. The FDA’s approval to commercially release recombinant glucocerebrosidase enzyme produced in carrot cells by Protalix Biotherapeutics was the first plant-produced biopharmaceutical to be released for human consumption into the market. This is a major achievement in the field of molecular pharming. Although many other biopharmaceuticals produced in plants are in the pipeline for commercial release after undergoing various stages of clinical trials, there is room for improvement in enhancing recombinant protein yield in plants by exploring strategies involving genetics, genomics, epigenetics, in silico, and purification techniques. In this chapter, we discuss various approaches of plant biotechnology and plant genetic engineering that are being used in the molecular pharming of biopharmaceuticals.
Abbreviations
- ADA:
-
Adenosine deaminase
- AGPs:
-
Arabinogalactan proteins
- BY-2:
-
Bright yellow-2
- CaMV:
-
Cauliflower mosaic virus
- CPMV:
-
Cowpea mosaic virus
- DNA:
-
Deoxyribonucleic acid
- ER:
-
Endoplasmic reticulum
- ERT:
-
Enzyme replacement therapy
- FDA:
-
Food and drug administration
- GBP-Fc:
-
Anti-green fluorescent protein antibody
- GMOs:
-
Genetically modified organisms
- HAC1:
-
Hemagglutinin antigen
- HDEL:
-
H-Histidine D-Aspartic acid, E-Glutamic acid, L-Leucine
- hGM-CSF:
-
Human Granulocyte-macrophage colony-stimulating factor
- HIV:
-
Human immunodeficiency virus
- KDEL:
-
K-Lysine D-Aspartic acid, E-Glutamic acid, L-Leucine
- MERS:
-
Middle East respiratory syndrome
- mRNA:
-
Messenger RNA
- Pphas:
-
β-phaseolin promoter
- PpsbA:
-
Photosystem II protein D1promoter
- PSAD:
-
Photosystem I subunit D1promoter
- PVP:
-
Polyvinylpyrrolidone
- PVX:
-
Potato Virus X
- RAmy3D:
-
Rice α-amylase 3D
- RNA:
-
Ribonucleic acid
- SCID:
-
Severe combined immune deficiency disorders
- TMV:
-
Tobacco mosaic virus
- USDA:
-
United States Department of Agriculture
- UTRs:
-
Untranslated regions
- VHH-Fc:
-
Nanobodies-crystallisable fragments
- VLP:
-
Virus-like particle
- VNPs:
-
Virus-based nanoparticles
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Singhabahu, S., Hefferon, K., Makhzoum, A. (2016). Transgenesis and Plant Molecular Pharming. In: Jha, S. (eds) Transgenesis and Secondary Metabolism. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-319-27490-4_21-2
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DOI: https://doi.org/10.1007/978-3-319-27490-4_21-2
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Latest
Transgenesis and Plant Molecular Pharming- Published:
- 06 February 2017
DOI: https://doi.org/10.1007/978-3-319-27490-4_21-3
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Transgenesis and Plant Molecular Pharming
- Published:
- 29 December 2016
DOI: https://doi.org/10.1007/978-3-319-27490-4_21-2
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Original
Plant Molecular Pharming- Published:
- 08 October 2016
DOI: https://doi.org/10.1007/978-3-319-27490-4_21-1