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Electron Paramagnetic Resonance Spectroscopy to Evaluate the Radical Scavenging Activity of the Skin

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Measuring the Skin

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Abstract

If an unbalance of oxidants and antioxidants appears in the skin, oxidative stress is the consequence, and an excess of reactive oxygen species can cause severe cell damage associated with skin carcinogenesis and accelerated skin aging. In this chapter, it is shown that electron paramagnetic resonance spectroscopy is able to measure the antioxidant status of the skin by investigating the radical scavenging activity of applied test radicals. The direct reaction of cutaneous antioxidants can be studied using the application of the test radical TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy), which is exponentially reduced. These reduction kinetics can be exponentially fitted and the resulting rate constant is a measure of the antioxidant status. Irradiation induced short-living radicals can be investigated by their competitive reaction between the applied test radical PCA (3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy) and the cutaneous antioxidants. It could be shown that orally and topically supplemented antioxidants increase the cutaneous antioxidant status. Systemic antioxidants applied in a physiological concentration can increase the antioxidant status by 50 %. Furthermore, compounds which increase the rate constant also provide protection against stress induced radicals as formed during VIS/NIR irradiation. Topical and oral application can be combined providing an optimum of protection against premature skin aging and development of cancer.

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Acknowledgments

This work was supported by the Federal Land of Berlin within the Program to Promote Research, Innovation and Technologies (ProFIT, grant n# 10142343 and 10149524) for granting subsidies from the innovation promotion fund, with cofinancing by the European Fund for Regional Development (ERDF). We would also like to thank the companies Privatinstitut Galenus, Bioactive Food, and Klosterfrau Healthcare Group for providing the investigated products containing the different antioxidants.

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Authors and Affiliations

  1. Department of Dermatology, Venereology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany

    Martina C Meinke, Anna-Christina Lauer, Annette Friedrich, Sophia Arndt, Stefan F Haag & Jürgen Lademann

Authors
  1. Martina C Meinke
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  2. Anna-Christina Lauer
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  3. Annette Friedrich
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  4. Sophia Arndt
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  5. Stefan F Haag
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  6. Jürgen Lademann
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Corresponding author

Correspondence to Martina C Meinke .

Editor information

Editors and Affiliations

  1. Medecine Vasculaire Lab. Ingénierie et de Biologie Cutanées, CHU Besancon Service Dermatologie et, Besancon, France

    Philippe Humbert

  2. San Francisco, California, USA

    Howard Maibach

  3. Department of Dermatology, Saint Jacques University Hospital, Besançon, France

    Ferial Fanian

  4. Univ. Besançon Dépt. Dermatologie, Besançon, France

    Pierre Agache

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Meinke, M.C., Lauer, AC., Friedrich, A., Arndt, S., Haag, S.F., Lademann, J. (2015). Electron Paramagnetic Resonance Spectroscopy to Evaluate the Radical Scavenging Activity of the Skin. In: Humbert, P., Maibach, H., Fanian, F., Agache, P. (eds) Measuring the Skin. Springer, Cham. https://doi.org/10.1007/978-3-319-26594-0_148-1

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  • DOI: https://doi.org/10.1007/978-3-319-26594-0_148-1

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  • Publisher Name: Springer, Cham

  • Online ISBN: 978-3-319-26594-0

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