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Sample Preparation Methods for the Rapid MS Analysis of Microorganisms

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Applications of Mass Spectrometry in Microbiology

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Abstract

Sample preparation is the first and the most crucial step in the identification of microorganisms. In this chapter, an overview is presented on different sample preparation steps used in the context of rapid analysis of microorganisms by mass spectrometry (MS). Sample preparation methods that might eliminate the need for culturing of the target cells (the key rate-limiting step) to obtain detectable signals are important for the rapid analysis of microorganisms. Microbial cell and biomarker enrichment through various affinity techniques are useful to enhance the sensitivity and accuracy of microbial identification. Combining the methodologies for cell lysis and biomarker solubilization will facilitate the rapid identification of microorganisms. Efficient biomarker separation techniques such as liquid chromatography may guarantee a more accurate MS identification of drug-resistance-related biomolecules in microbial mixtures or complex biological samples. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is the method of choice for the rapid identification of microorganisms. This chapter concludes with a brief discussion of the selection of MALDI matrices and matrix solvents.

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Authors and Affiliations

  1. Department of Chemistry, National Dong Hwa University, 97401, Hualien, Taiwan

    C. Shobha Devi & Yen-Peng Ho

  2. Institute of Medical Biotechnology, Tsu-Chi University, Hualien, Taiwan

    Anren Hu

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  1. C. Shobha Devi
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  2. Anren Hu
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Correspondence to Yen-Peng Ho .

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Editors and Affiliations

  1. Johns Hopkins University, Applied Physics Laboratory, Laurel, Maryland, USA

    Plamen Demirev

  2. Arizona State University, School of Math and Natural Sciences, Phoenix, Arizona, USA

    Todd R. Sandrin

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Devi, S., Hu, A., Ho, YP. (2016). Sample Preparation Methods for the Rapid MS Analysis of Microorganisms. In: Demirev, P., Sandrin, T. (eds) Applications of Mass Spectrometry in Microbiology. Springer, Cham. https://doi.org/10.1007/978-3-319-26070-9_3

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