Abstract
Sweet potatoes (Ipomoea batatas) were investigated as a basic component to develop a medium for the cultivation of lactobacilli. Extract from baked sweet potatoes was used to form a sweet potato medium (SPM) which was supplemented with 0, 4, or 8 g/L of each nitrogen source (beef extract, yeast extract, and proteose peptone #3) to develop SPM1, SPM2, and SPM3, respectively. The growth of Lactobacillus in SPM was compared to lactobacilli MRS. Low inoculums’ levels (2–2.5 log CFU/mL) were used to investigate the suitability of SPM to support the growth of Lactobacillus. Lactobacillus strains were individually inoculated into batches of MRS, SPM1, SPM2, and SPM3 then incubated at 37 °C. The growth of Lactobacillus was monitored using turbidity (OD at 610 nm), bacterial population (log CFU/mL), and pH values. Our results showed no significant differences (p < 0.05) in the maximum specific growth rates (μ max) of Lactobacillus strains growing in MRS, SPM2, and SPM3. After 24 h on incubation, Lactobacillus strains grown in SPM2, SPM3, and MRS reached averages of 10.59 ± 0.27, 10.72 ± 0.19, and 10.41 ± 0.35 log CFU/mL, respectively. Slower growth rates were observed in SPM1 with 1.57 ± 0.55 log CFU/mL less of bacterial populations than MRS. SPM2 and SPM3 maintained higher pH values throughout the incubation period compared to MRS. Therefore, these findings indicated that SPM2, containing 12 g/L of nitrogen sources, is suitable for the growth of Lactobacillus, and SPM2 could be used as an alternative low-cost medium.
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References
Altaf, M. D., Naveena, B. J., & Reddy, G. (2007). Use of inexpensive nitrogen sources and starch for L (+) lactic acid production in anaerobic submerged fermentation. Bioresource Technology, 98(3), 498–503.
Djeghri-Hocine, B., Boukhemis, M., Zidoune, M. N., & Amrane, A. (2007). Evaluation of de-lipidated egg yolk and yeast autolysate as growth supplements for lactic acid bacteria culture. International Journal of Dairy Technology, 60(4), 292–296.
Duda-Chodak, A., Tarko, T., & Statek, M. (2008). The effect of antioxidants on Lactobacillus casei cultures. ACTA Scientiarum Polonorum Technologia Alimentaria, 7, 39–51.
Hayek, S. A., & Ibrahim, S. A. (2013). Current limitations and challenges with lactic acid bacteria: A review. Food and Nutrition Sciences, 4(11A), 73–87.
Hébert, E. M., Raya, R. R., & de Giori, G. S. (2004). Evaluation of minimal nutritional requirements of lactic acid bacteria used in functional foods. In J. M. Walker, J. F. Spencer, & A. L. Ragout de Spencer (Eds.), Methods in biotechnology (pp. 139–150). Totowa, NJ: Humana Press.
Horn, S. J., Aspmo, S. I., & Eijsink, V. G. H. (2005). Growth of Lactobacillus plantarum in media containing hydrolysates of fish viscera. Journal of Applied Microbiology, 99, 1082–1089. doi:10.1111/j.1365-2672.2005.02702.x.
Jenkins, J. K., & Courtney, P. D. (2003). Lactobacillus growth and membrane composition in the presence of linoleic or conjugated linoleic acid. Canadian Journal of Microbiology, 49(1), 51–57.
Lechiancole, T., Ricciardi, A., & Parente, E. (2002). Optimization of media and fermentation conditions for the growth of Lactobacillus sakei. Annals of Microbiology, 52(3), 257–274.
Lucier, G., & Glaser, L. (2011). Vegetables and melons outlook. USDA, A Report from the Economic Service VGS-343 (pp. 1–47).
Padmaja, G. (2009). Uses and nutritional data of sweetpotato. In G. Loebenstein & G. Thottappilly (Eds.), The sweetpotato (Vol. 1, pp. 189–234). Dordrecht, The Netherlands: Springer.
Salaün, F., Mietton, B., & Gaucheron, F. (2005). Buffering capacity of dairy products. International Dairy Journal, 15(2), 95–109.
Sawatari, Y., Hirano, T., & Yokota, A. (2006). Development of food grade media for the preparation of Lactobacillus plantarum starter culture. The Journal of General and Applied Microbiology, 52(6), 349–356.
Song, D., Ibrahim, S., & Hayek, S. (2012). Recent application of probiotics in food and agricultural science. In E. C. Rigobelo (Ed.), Probiotics (Vol. 10, pp. 1–34). Manhattan, NY: InTech.
USDA. (2012). Nutrient data for 11508, sweet potato, cooked, baked in skin, without salt. Nutrient Data Laboratory. Washington, DC: U.S. Department of Agriculture.
Vazquez, J. A., Gonzalez, M. P., & Murado, M. A. (2004). Peptones from autohydrolysed fish viscera for nisin and pediocin production. Journal of Biotechnology, 112(3), 299–311.
Vera Pingitore, E., Hebert, E. M., Sesma, F., & Nader-Macias, M. E. (2009). Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium. Canadian Journal of Microbiology, 55(3), 304–310. doi:10.1139/w08-092.
Von Wright, A., & Axelsson, L. (2011). Lactic acid bacteria: An introduction. In S. Lahtinne, S. Salminen, A. Von Wright, & A. Ouwehand (Eds.), Lactic acid bacteria: Microbiological and functional aspects (pp. 1–17). London: CRC Press.
Wegkamp, A., Teusink, B., De Vos, W. M., & Smid, E. J. (2010). Development of a minimal growth medium for Lactobacillus plantarum. Letters in Applied Microbiology, 50(1), 57–64.
Acknowledgments
This work was made possible by grant number NC.X-267-5-12-170-1 from the National Institute of Food and Agriculture and its contents are solely the responsibility of the authors and do not necessarily represent the official view of the National Institute of Food and Agriculture. The full paper of this work was published in the journal of Bioscience, Biotechnology, and Biochemistry, 2013, 77(11), 2248–2254, and part was presented at the 17th Biennial Research Symposium of the Association of 1890 Research Directors, Inc. (ARD) April, 2013.
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Hayek, S.A., Shahbazi, A., Ibrahim, S.A. (2016). Using Sweet Potatoes as a Basic Component to Develop a Medium for the Cultivation of Lactobacilli. In: Uzochukwu, G., Schimmel, K., Kabadi, V., Chang, SY., Pinder, T., Ibrahim, S. (eds) Proceedings of the 2013 National Conference on Advances in Environmental Science and Technology. Springer, Cham. https://doi.org/10.1007/978-3-319-19923-8_15
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