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Application of Chromosomal Microarray

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Molecular Oncology Testing for Solid Tumors

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Abstract

Recognition of cytogenetic abnormalities in solid tumors is important to aid in decisions regarding the diagnosis, prognosis, and treatment of patients. Data obtained from DNA copy number microarray methodologies is allowing for heretofore unprecedented accuracy in identifying chromosomal anomalies. This chapter reviews pragmatic aspects of the application of DNA copy number microarray studies for genome-wide assessments of solid tumors, including discussions of the laboratory and analytic processes, the nomenclature used for reporting, and the selection of specimens that are optimal for testing. A review of the extant literature summarizing imbalances that have been recognized, to date, from the application of this technology to a variety of solid tumors is also presented, along with a review of regions with loss of heterozygosity that have been associated with solid tumors using single nucleotide polymorphism (SNP) microarray platforms. Strengths, weaknesses, and applications of this methodology, primarily from a clinical application perspective, are also discussed.

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Acknowledgements

The authors thank Noran Aboalela, Ph.D., Steven Smith, M.D. Ph.D., Ema Dragoescu, M. D., Priscilla Gonzalez, Cynthia Urick, and Catherine Ventura for their assistance in the procurement and assessment of the specimens featured in the figures accompanying this chapter. They also thank Jennifer Dewitt for her assistance in preparing this manuscript.

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Authors and Affiliations

  1. Departments of Pathology, Human & Molecular Genetics, Massey Cancer Center, Virginia Commonwealth University, 980662, Richmond, VA, 23298, USA

    Colleen Jackson-Cook Ph.D., F.A.C.M.G.

  2. Department of Pathology, Virginia Commonwealth University, 980662, Richmond, VA, 23298, USA

    Shivani Ponnala Ph.D.

  3. Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA

    Shivani Ponnala Ph.D.

Authors
  1. Colleen Jackson-Cook Ph.D., F.A.C.M.G.
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  2. Shivani Ponnala Ph.D.
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Correspondence to Colleen Jackson-Cook Ph.D., F.A.C.M.G. .

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Editors and Affiliations

  1. Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, USA

    Michael Ola Idowu

  2. Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, USA

    Catherine Isabelle Dumur

  3. Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, USA

    Carleton Theodore Garrett

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Jackson-Cook, C., Ponnala, S. (2015). Application of Chromosomal Microarray. In: Idowu, M., Dumur, C., Garrett, C. (eds) Molecular Oncology Testing for Solid Tumors. Springer, Cham. https://doi.org/10.1007/978-3-319-16304-8_7

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  • DOI: https://doi.org/10.1007/978-3-319-16304-8_7

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