Abstract
In this study, the in vitro and in vivo anti-inflammatory and antioxidant potentials of abalone (Haliotis discus) water extract were investigated. The taurine concentration of the abalone water extract was 593.80 nmol/mg, which was represented the most abundant total free amino acid. Abalone (Haliotis discus) water extract is a potent scavenger of free radicals, including DPPH, superoxide and alkyl radicals, which were investigated using a spin-trapping electron method and electron spin resonance (ESR) signal intensity. In addition, abalone (Haliotis discus) water extract showed a protective effect against 2,2′-azobis dihydrochloride (AAPH)-induced oxidative stress and cell death in the in vivo zebrafish model. Furthermore, we evaluated the anti-inflammatory effect of abalone water extract using the lipopolysaccharide (LPS)-stimulated zebrafish model. Abalone water extract markedly inhibited LPS-stimulated ROS and NO generation. In this study, it is proved that both the in vitro and in vivo anti-inflammatory as well as antioxidant potentials of abalone water extract, which contains taurine could be further utilized in relevant industrial applications.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Abbreviations
- AAPH:
-
2,2′-azobis dihydrochloride
- DPPH:
-
1,1-diphenyl-2-picrylhydrazyl
- ESR:
-
electron spin resonance
- LPS:
-
lipopolysaccharide
- NO:
-
nitric oxide
- ROS:
-
reactive oxygen species
References
Balkan J, Kanbagli O, Hatipoglu A, Kucuk M, Cevikbas U, Aykac-Toker G, Uysal M (2002) Improving effect of dietary taurine supplementation on the oxidative stress and lipid levels in the plasma, liver and aorta of rabbits fed on a high-cholesterol diet. Biosci Biotechnol Biochem 66:1755–1758. doi:10.1271/bbb.66.1755
Bhavsar TM, Cantor JO, Patel SN, Lau-Cam CA (2009) Attenuating effect of taurine on lipopolysaccharide-induced acute lung injury in hamsters. Pharmacol Res 60:418–428. doi:10.1016/j.phrs.2009.05.006
Bircan FS, Balabanli B, Turkozkan N, Ozan G (2011) Effects of taurine on nitric oxide and 3-nitrotyrosine levels in spleen during endotoxemia. Neurochem Res 36:1978–1983. doi:10.1007/s11064-011-0521-3
Camuesco D, Comalada M, Rodriguez-Cabezas ME, Nieto A, Lorente MD, Concha A, Zarzuelo A, Gálvez J (2004) The intestinal anti-inflammatory effect of quercitrin is associated with an inhibition in iNOS expression. Br J Pharmacol 143:908–918. doi:10.1038/sj.bjp.0705941
Cetiner M, Sener G, Sehirli AO, Eksioglu-Demiralp E, Ercan F, Sirvaci S, Gedikf N, Akpulatc S, Tecimerc T, Yeğen BÇ (2005) Taurine protects against methotrexate-induced toxicity and inhibits leucocyte death. Toxicol Appl Pharmacol 209:39–50. doi:10.1016/j.taap.2005.03.009
Chen C, Chi YJ, Zhao MY, Lv L (2012) Purification and identification of antioxidant peptides from egg white protein hydrolysate. Amino Acids 43:457–466. doi:10.1007/s00726-011-1102-0
Eisen JS (1996) Zebrafish make a big splash. Cell 87:969–977. doi:10.1016/S0092-8674(00)81792-4
Emmanouil C, Green RM, Willey FR, Chipman JK (2008) Oxidative damage in gill of Mytilus edulis from Merseyside, UK, and reversibility after depuration. Environ Pollut 151:663–668. doi:10.1016/j.envpol.2007.03.013
Grimble RF (1996) Theory and efficacy of antioxidant therapy. Curr Opin Crit Care 2:260–266
Hansen SH (2001) The role of taurine in diabetes and the development of diabetic complications. Diabetes Metab Res Rev 17:330–346. doi:10.1002/dmrr.229
Hibbs JB Jr, Taintor RR, Vavrin Z, Rachlin EM (1988) Nitric oxide: a cytotoxic activated macrophage effector molecule. Biochem Biophys Res Commun 157:87–94. doi:10.1016/S0006-291X(88)80015-9
Huxtable RJ (1996) Taurine past, present, and future. Adv Exp Med Biol 403:641–650. doi:10.1007/978-1-4899-0182-8_71
Itoh Y, Ma FH, Hoshi H, Oka M, Noda K, Ukai Y, Kojima H, Nagano T, Toda N (2000) Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry. Anal Biochem 287:203–209. doi:10.1006/abio.2000.4859
Kalaz EB, Çoban J, Aydın AF, Doğan-Ekici I, Doğru-Abbasoğlu S, Öztezcan S, Uysal M (2014) Carnosine and taurine treatments decreased oxidative stress and tissue damage induced by d-galactose in rat liver. J Physiol Biochem 70:15–25. doi:10.1007/s13105-013-0275-2
Kang MC, Cha SH, Wijesinghe WAJP, Kang SM, Lee SH, Kim EA, Song CB, Jeon YJ (2013) Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo. Food Chem 138:950–955. doi:10.1016/j.foodchem.2012.11.005
Lin NT, Yang FL, Lee RP, Peng TC, Chen HI (2006) Inducible nitric oxide synthase mediates cytokine release: the time course in conscious and septic rats. Life Sci 78:1038–1043. doi:10.1016/j.lfs.2005.05.091
Lu JM, Lin PH, Yao Q, Chen C (2010) Chemical and molecular mechanisms of antioxidant: experimental approaches and model system. J Cell Mol Med 14:840–860. doi:10.1111/j.1582-4934.2009.00897.x
Marcinkiewicz J, Grabowska A, Bereta J, Stelmaszynska T (1995) Taurine chloramine, a product of activated neutrophils, inhibits in vitro the generation of nitric oxide and other macrophage inflammatory mediators. J Leukoc Biol 58:667–674
Molina PE, Qian L, Schuhlein D, Naukam R, Wang H, Tracey KJ, Abumrad NN (1998) CNI-1493 attenuates hemodynamic and pro-inflammatory responses to LPS. Shock 10:329–334
Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y (1996) Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radic Biol Med 21:895–902. doi:10.1016/0891-5849(96)00237-7
Oliveira MW, Minotto JB, de Oliveira MR, Zanotto-Filho A, Behr GA, Rocha RF, Moreira JC, Klamt F (2010) Scavenging and antioxidant potential of physiological taurine concentrations against different reactive oxygen/nitrogen species. Pharmacol Rep 62:185–193. doi:10.1016/S1734-1140(10)70256-5
Peng X, Xiong YL, Kong B (2009) Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance. Food Chem 113:196–201. doi:10.1016/j.foodchem.2008.07.068
Redmond HP, Stapleton PP, Neary P, Bouchier-Hayes D (1998) Immunonutrition: the role of taurine. Nutrition 14(7–8):599–604. doi:10.1016/S0899-9007(98)00097-5
Rosemberg DB, Da Rocha RF, Rico EP, Zanotto-Filho A, Dias RD, Bogo MR, Bonan CD, Moreira JC, Klamt F, Souza DO (2010) Taurine prevents enhancement of acetylcholinesterase activity induced by acute ethanol exposure and decreases the level of markers of oxidative stress in zebrafish brain. Neuroscience 171:683–692. doi:10.1016/j.neuroscience.2010.09.030
Rosenkranz AR, Schmaldienst S, Stuhlmeier KM, Chen W, Knapp W, Zlabinger GJ (1992) A micro plate assay for the detection of oxidative products using 2′,7′-dichlorofluorescein-diacetate. J Immunol Methods 156:39–45. doi:10.1016/0022-1759(92)90008-H
Schaffer SW, Azuma J, Mozaffari M (2009) Role of antioxidant activity of taurine in diabetes. Can J Physiol Pharmacol 87:91–99. doi:10.1139/Y08-110
Stocker R, Keaney JF (2004) Role of oxidative modifications in atherosclerosis. Physiol Rev 84:1381–1478. doi:10.1152/physrev.00047.2003
Takamatsu S, Hodges TW, Rajbhandari I, Gerwick WH, Hamann MT, Nagle DG (2003) Marine natural products as novel antioxidant prototypes. J Nat Prod 66:605–608. doi:10.1021/np0204038
Utkina N, Makarchenko A, Shchelokova O, Virovaya M (2004) Antioxidant activity of phenolic metabolites from marine sponges. Chem Nat Compd 40:373–377. doi:10.1023/B:CONC.0000048251.58246.22
Wang B, Li L, Chi CF, Ma JH, Luo HY, Xu YF (2013) Purification and characterisation of a novel antioxidant peptide derived from blue mussel (Mytilus edulis) protein hydrolysate. Food Chem 138:1713–1719. doi:10.1016/j.foodchem.2012.12.002
Xiao C, Giacca A, Lewis GF (2008) Oral taurine but not N-acetylcysteine ameliorates NEFA-induced impairment in insulin sensitivity and beta cell function in obese and overweight, non-diabetic men. Diabetologia 51:139–146. doi:10.1007/s00125-007-0859-x
Yang CS, Landau JM, Huang MT, Newmark HL (2001) Inhibition of carcinogenesis by dietary polyphenolic compounds. Annu Rev Nutr 21:381–406. doi:10.1146/annurev.nutr.21.1.381
Zhu X, Raina AK, Lee HG, Casadesus G, Smith MA, Perry G (2004) Oxidative stress signalling in Alzheimer’s disease. Brain Res 1000:32–39. doi:10.1016/j.brainres.2004.01.012
Acknowledgements
This study was supported by a grant from the Korea Food Research Institute (Project name: Development of silver foods for the elderly).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this paper
Cite this paper
Cheong, S.H. et al. (2015). In Vitro and In Vivo Antioxidant and Anti-inflammatory Activities of Abalone (Haliotis discus) Water Extract. In: Marcinkiewicz, J., Schaffer, S. (eds) Taurine 9. Advances in Experimental Medicine and Biology, vol 803. Springer, Cham. https://doi.org/10.1007/978-3-319-15126-7_67
Download citation
DOI: https://doi.org/10.1007/978-3-319-15126-7_67
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-15125-0
Online ISBN: 978-3-319-15126-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)