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Nonlinear Fourier-Transform Spectroscopy Using Ultrabroadband Femtosecond Pulses for the Measurement of Photobleaching of Fluorescent Proteins

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Ultrafast Phenomena XIX

Part of the book series: Springer Proceedings in Physics ((SPPHY,volume 162))

Abstract

We investigate the mechanism of photobleaching of fluorescent proteins using nonlinear Fourier-transform spectroscopy with ultrabroadband femtosecond pulses. Photobleaching of fluorescent molecules occurs through one-photon excited-state absorption following two-photon excitation from the ground state.

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References

  1. W. R. Zipfel, R. M. Williams and W. W. Webb, Nat. Biotec. 21, 1369-1377 (2003).

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  2. H. Hashimoto, K. Isobe, A. Suda, F. Kannari, H. Kawano, H. Mizuno, A. Miyawaki, and K. Midorikawa, Appl. Opt. 49, 3323-3329 (2010).

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Acknowledgements

We would like to thank Drs. K. Isobe, K. Midorikawa, and A. Miyawaki of RIKEN, and Prof. F. Kannari of Keio Univ. for their support and cooperation during the work. This work was supported in part by the Grants-in-Aid for Scientific Research (No. 24560052) and the Strategic Research Foundation Grant-aided Project for Private Universities, both from the MEXT, Japan.

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Authors and Affiliations

  1. Department of Physics, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan

    Akira Suda, Hiroshi Takahashi & Keisuke Toda

Authors
  1. Akira Suda
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  2. Hiroshi Takahashi
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  3. Keisuke Toda
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Corresponding author

Correspondence to Akira Suda .

Editor information

Editors and Affiliations

  1. Department of Chemistry, The University of Tokyo, Tokyo, Japan

    Kaoru Yamanouchi

  2. JILA, University of Colorado, Boulder, Colorado, USA

    Steven Cundiff

  3. Department of Chemistry, Ludwig-Maximilians-University, München, Germany

    Regina de Vivie-Riedle

  4. Department of Physics, The University of Tokyo, Tokyo, Japan

    Makoto Kuwata-Gonokami

  5. Department of Physics, The Ohio State University, Columbus, Ohio, USA

    Louis DiMauro

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Suda, A., Takahashi, H., Toda, K. (2015). Nonlinear Fourier-Transform Spectroscopy Using Ultrabroadband Femtosecond Pulses for the Measurement of Photobleaching of Fluorescent Proteins. In: Yamanouchi, K., Cundiff, S., de Vivie-Riedle, R., Kuwata-Gonokami, M., DiMauro, L. (eds) Ultrafast Phenomena XIX. Springer Proceedings in Physics, vol 162. Springer, Cham. https://doi.org/10.1007/978-3-319-13242-6_133

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