Abstract
We have developed a robust cell patterning method using micromolding in capillaries (MIMIC) and self-assembled crystalline bacterial protein (S-layer) monolayers. The unique self-assembly properties of proteins SbpA and SbsB are exploited to create an anisotropic protein nanobiointerface with spatially-defined cytophilic (adhesive) and cytophobic (repulsive) properties. The chosen S-layer proteins were characterized using atomic force microscopy (AFM), zeta-potential and contact angle measurements. Results revealed distinct differences in surface topography and physico-chemical properties between SbsB and SbpA monolayers. Stability testing of cytophobic protein SbpA using vacuum-drying deposition and re-assembly on-flow sowed no remarkable differences with respect to the antifouling properties. Finally, using MIMIC micropatterns of epithelial CaCo-2 cell as well as mobile Jurkat cells were established using anisotropic S-layer protein monolayers.
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© 2015 Springer International Publishing Switzerland
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Rothbauer, M., Küpcü, S., Sleytr, U.B., Ertl, P. (2015). Crystalline Bacterial Protein Nanolayers for Cell Micropatterning. In: Lacković, I., Vasic, D. (eds) 6th European Conference of the International Federation for Medical and Biological Engineering. IFMBE Proceedings, vol 45. Springer, Cham. https://doi.org/10.1007/978-3-319-11128-5_84
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DOI: https://doi.org/10.1007/978-3-319-11128-5_84
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-11127-8
Online ISBN: 978-3-319-11128-5
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