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Electrochemical Antibody-Aptamer Assay for VEGF Cancer Biomarker Detection

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Sensors and Microsystems

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 268))

Abstract

In this work, a simple and sensitive approach for VEGF detection using antibody-aptamer assay and gold screen-printed electrodes as transducers is presented. The assay was performed in a sandwich format. Anti-VEGF antibody was first chemically immobilized on the gold working electrode surface of screen-printed cell. After the incubation with the antigen, the sandwich assay was realized by incubation step with biotinylated anti-VEGF aptamer. The sensor was then incubated with streptavidin-alkaline phosphatase and with 1-naphthyl phosphate. Differential pulse voltammetry (DPV) measurements were performed to detect VEGF biomarker.

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Author information

Authors and Affiliations

  1. Department of Chemistry “Ugo Schiff”, University of Florence, Via della Lastruccia 3, 50019, Sesto Fiorentino, Florence, Italy

    Andrea Ravalli & Giovanna Marrazza

  2. Nanobioelectronics & Biosensors Group, CIN2 (ICN-CSIC), Catalan Institute of Nanotechnology, UAB Campus, Bellaterra, Barcelona, Spain

    Lourdes Rivas, Alfredo De La Escosura-Muniz & Arben Merkoci

Authors
  1. Andrea Ravalli
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  2. Giovanna Marrazza
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  3. Lourdes Rivas
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  4. Alfredo De La Escosura-Muniz
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  5. Arben Merkoci
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Corresponding author

Correspondence to Giovanna Marrazza .

Editor information

Editors and Affiliations

  1. Dept. of Electronic Engineering, University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  2. Department of Information Engineering, University of Brescia, Brescia, Italy

    Vittorio Ferrari

  3. SENSOR Laboratory, IDASC, National Research Council (CNR), Brescia, Italy

    Andrea Ponzoni

  4. Department of Information Engineering, University of Brescia, Brescia, Italy

    Giorgio Sberveglieri

  5. Department of Information Engineering, University of Brescia, Brescia, Italy

    Marco Ferrari

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© 2014 Springer International Publishing Switzerland

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Ravalli, A., Marrazza, G., Rivas, L., De La Escosura-Muniz, A., Merkoci, A. (2014). Electrochemical Antibody-Aptamer Assay for VEGF Cancer Biomarker Detection. In: Di Natale, C., Ferrari, V., Ponzoni, A., Sberveglieri, G., Ferrari, M. (eds) Sensors and Microsystems. Lecture Notes in Electrical Engineering, vol 268. Springer, Cham. https://doi.org/10.1007/978-3-319-00684-0_33

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  • DOI: https://doi.org/10.1007/978-3-319-00684-0_33

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-00683-3

  • Online ISBN: 978-3-319-00684-0

  • eBook Packages: EngineeringEngineering (R0)

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