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Quantitative Hematocrit Measurement on a Pressure-Actuated Microfluidic Chip

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12th Asian-Pacific Conference on Medical and Biological Engineering (APCMBE 2023)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 103))

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Abstract

In point-of-care diagnostics, hematocrit (HCT), which is the volume percentage of red blood cells in the whole blood sample, is closely related to human health status. Here, we developed a pressure-actuated, polydimethylsiloxane (PDMS) microfluidic chip for quantitative measurement of HCT. The narrowest part of its flow channel is 8 μm and acts as a constriction for blood cells, which can lead to retardance of blood flow in the chip. The amount of red blood cells has an influence on the transport time of passing through the constriction for the blood samples, so we can use the transport time to infer the HCT value. We found a linear calibration curve between blood transport time and HCT value, which showed a correlation coefficient (R2) of 0.9506 for blood samples with HCT ranging from 0.35 to 0.55. This microfluidic chip can provide the results of HCT measurement within 1 min, making it well-suited for point-of-care diagnostics.

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Acknowledgment

This research was funded by Shantou University (STU Scientific Research Foundation for Talents: NTF20034), Department of Education of Guangdong Province (Scientific Research Project: 2022KQNCX019), and Guangdong Basic and Applied Basic Research Foundation (2022A1515110855).

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Authors and Affiliations

  1. Department of Electronic Engineering, Shantou University, Shantou, China

    Haonan Li & Muyang Zhang

  2. Department of Biology, Shantou University, Shantou, China

    Zejingqiu Chen

  3. Department of Biomedical Engineering, Shantou University, 243 Daxue Road, Shantou, China

    Zhiqing Xiao, Zitao Feng, Eric S. Hald & Weijin Guo

Authors
  1. Haonan Li
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  2. Muyang Zhang
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  3. Zejingqiu Chen
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  4. Zhiqing Xiao
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  5. Zitao Feng
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  6. Eric S. Hald
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  7. Weijin Guo
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Corresponding author

Correspondence to Weijin Guo .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China

    Guangzhi Wang

  2. School of Life Science and Technology, University of Electronic Science and Technology, Chengdu, China

    Dezhong Yao

  3. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

    Zhongze Gu

  4. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine Peking Union Medical College, Beijing, China

    Yi Peng

  5. School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China

    Shanbao Tong

  6. State Key Laboratory of Bioelectronics, School of Instrument Science and Engineering, Southeast University, Nanjing, China

    Chengyu Liu

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© 2024 The Author(s), under exclusive license to Springer Nature Switzerland AG

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Li, H. et al. (2024). Quantitative Hematocrit Measurement on a Pressure-Actuated Microfluidic Chip. In: Wang, G., Yao, D., Gu, Z., Peng, Y., Tong, S., Liu, C. (eds) 12th Asian-Pacific Conference on Medical and Biological Engineering. APCMBE 2023. IFMBE Proceedings, vol 103. Springer, Cham. https://doi.org/10.1007/978-3-031-51455-5_46

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  • DOI: https://doi.org/10.1007/978-3-031-51455-5_46

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-031-51454-8

  • Online ISBN: 978-3-031-51455-5

  • eBook Packages: EngineeringEngineering (R0)

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