Abstract
Electrochemotherapy (ECT) is a cancer treatment that uses electroporation (EP) to boost chemotherapeutic drug effectiveness due to increased cell membrane permeability. The membrane effect is a phenomenon of incremented conductivity increasing beneath consecutive pulses. This preliminary study tested whether the membrane effect would occur between three consecutive ESOPE protocol applications. Further, we analyzed if it would affect the safety of ECT treatments by unsafely increasing electrical currents in the tissue. Our findings showed that electroporated membranes did not fully recover in twenty seconds after an ECT protocol. The phenomenon was well observed at the ECT levels. Still, it had a minor influence on conductivity rising, not representing a risk to ECT safety.
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Acknowledgements
The authors would like to thank the Central Animal Facility-UFSC for technical support of animals care management used on this work. This study was financed in part by the Coordenačão de Aperfeičoamento de Pessoal de Nível Superior - Brasil (CAPES). The authors would like to thank the Brazilian research funding agencies CAPES and CNPq for the scholarships granted to the post-graduate students participating in the study and for the Programa Institucional de Bolsas de Iniciačão Científica (PIBIC) scholarship granted to the undergraduate student.
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Paro, I.B., Lopes, L.B., Andrade, D.L.L.S., Lorenzo, M.A., Suzuki, D.O.H. (2024). Evaluation of Electroporation Memory in Electrochemotherapy Protocols: Ex Vivo Liver Preliminary Study. In: Marques, J.L.B., Rodrigues, C.R., Suzuki, D.O.H., Marino Neto, J., García Ojeda, R. (eds) IX Latin American Congress on Biomedical Engineering and XXVIII Brazilian Congress on Biomedical Engineering. CLAIB CBEB 2022 2022. IFMBE Proceedings, vol 98. Springer, Cham. https://doi.org/10.1007/978-3-031-49401-7_35
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DOI: https://doi.org/10.1007/978-3-031-49401-7_35
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