Abstract
Platelet-enriched plasma is autologous blood product, that contains platelets concentration two to three times above normal blood level. As such, PRP is a rich source of different bioactive molecules including grow factors, enzymes, cytokines, and chemokines. Beside its regenerative properties, a limited number of studies has proven that PRP can induce antimicrobial effect against single growing pathogens and biofilms. Aim of this study was to analyze PRP antimicrobial effect against three most common biofilm forming bacteria, including S. aureus, E. coli and P. aeruginosa. The antimicrobial property of PRP was evaluated after 24 h of incubation with selected bacteria in BHI media using spectrophotometer with a light source of 600 nm. To check whether PRP can inhibit bacterial biofilm formation, after 24 h incubation, tube screening test (TM) was applied. Bacteria treated with PRP and platelet poor plasma (PPP) were compared with untreated control, composed of bacteria growing spontaneously in BHI media. PRP produced strong growth inhibition in all tested bacteria when compared to bacteria treated with PPP and control group. Based on the obtained results it can be concluded that PRP can induce antimicrobial effect on S. aureus, P. aeruginosa and E. coli. PRP also reduced biofilm formation for P. aeruginosa and E. coli. However, there was no effect on S. aureus biofilm formation.
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References
Wasterlain, A.S., Braun, H.J., Dragoo, J.L.: Contents and formulations of platelet rich plasma. In: Maffulli, N. (eds.) Platelet Rich Plasma in Musculoskeletal Practice. Springer, London (2016)
Yeaman, M.R.: Platelets: at the nexus of antimicrobial defence. Nat. Rev. Microbiol. 12(6), 426–437 (2014)
Stoodley, P., Sauer, K., Davies, D.G., Costerton, J.W.: Biofilms as complex differentiated communities. Annu. Rev. Microbiol. 56, 187–209 (2002)
Çetinkaya, R.A., et al.: Platelet-rich plasma as an additional therapeutic option for infected wounds with multi-drug resistant bacteria: in vitro antibacterial activity study. Eur. J. Trauma Emerg. Surg. 45(3), 555–565 (2019)
Cieślik-Bielecka, A., Bold, T., Ziółkowski, G., Pierchała, M., Królikowska, A., Reichert, P.: Antibacterial activity of leukocyte- and platelet-rich plasma: an in vitro study. Biomed. Res. Int. 27(2018), 947–1723 (2018)
Maghsoudi, O., Ranjbar, R., Mirjalili, S.H., Fasihi-Ramandi, M.: Inhibitory activities of platelet-rich and platelet-poor plasma on the growth of pathogenic bacteria. Iran. J. Pathol. 12(1), 79–87 (2017)
Smith, O.J., Wicaksana, A., Davidson, D., Spratt, D., Mosahebi, A.: An evaluation of the bacteriostatic effect of platelet-rich plasma. Int. Wound J. 18(4), 448–456 (2021)
Gilbertie, J.M., et al.: Platelet-rich plasma lysate displays antibiofilm properties and restores anti-microbial activity against synovial fluid biofilms in vitro. J. Orthop. Res. 38(6), 1365–1374 (2020)
Christensen, G.D., Simpson, W.A., Bisno, A.L., Beachey, E.H.: Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect. Immun. 37(1), 318–326 (1982)
Gupta, S., Paliczak, A., Delgado, D.: Evidence-based indications of platelet-rich plasma therapy. Expert Rev. Hematol. 14(1), 97–108 (2021)
Dadgostar, P.: Antimicrobial resistance: implications and costs. Infect Drug Resist. 20(12), 3903–3910 (2019)
Wolcott, R.D., et al.: Analysis of the chronic wound microbiota of 2,963 patients by 16S rDNA pyrosequencing. Wound Repair Regen. 24(1), 163–174 (2016)
Tong, S.Y., Davis, J.S., Eichenberger, E., Holland, T.L., Fowler, V.G., Jr.: Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin. Microbiol. Rev. 28(3), 603–661 (2015)
Mariani, E., et al.: Platelet-rich plasma affects bacterial growth in vitro. Cytotherapy 16(9), 1294–1304 (2014)
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We thank for financial support to Ministry of Science, Higher Education and Youth of Canton Sarajevo, Bosnia and Herzegovina (Funding ID: 27–02-35–35137-41/22).
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Bećirević, T. et al. (2024). Evaluation of Platelet-Enriched Plasma Antimicrobial Effect: In Vitro Study. In: Badnjević, A., Gurbeta Pokvić, L. (eds) MEDICON’23 and CMBEBIH’23. MEDICON CMBEBIH 2023 2023. IFMBE Proceedings, vol 94. Springer, Cham. https://doi.org/10.1007/978-3-031-49068-2_3
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