Abstract
Celandine (Chelidonium majus) is herbaceous, medicinal herb used in traditional medicine for internal use as tea, syrup, as well for external use. Teas from celandine contain phenolic compounds with high antioxidant activity which creates positive effects on human health.
The aim of this study was to determine the content of phenolic compounds and antioxidant activity in fresh and dry parts of plants (leaf and stem) with using water, diluted methanol, diluted ethanol (as solvents), for 15 min at 70 °C. The samples of plant Celandine (Chelidonium majus) originates from Canton Sarajevo, Bosnia and Herzegovina. Total phenols compounds were determined by Folin-Ciocalteu method (600 nm), antioxidant activity was determined by using PFRAP (700 nm). Gallic acid was used as the standard, and the results are expressed in mg GAE/100 g. The results showed that fresh leaves have a higher content compared to samples of dried leaf, particularly when diluted ethanol was used as a solvent. Based on the obtained results it can be concluded that the samples of dried leaf have higher antioxidant activity in comparison to fresh leaf, in case when diluted methanol was used as a solvent. Also, fresh and dry Celandine leaf have higher phenolic content and antioxidant activity compared to fresh and dry stem. During the extraction it was proved that both fresh and dry parts (leaves and stem) showed the lowest phenolic compounds content and antioxidant activity when water was used as a solvent.
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Ašimović, Z., Hasanović, S., Muminović, Š., Spaho, N., Čorbo, S., Čengić, L. (2023). Antioxidant Activity and Total Phenolic Content Determining in Samples of Celandine (Chelidonium Majus L.) by Applying Different Solvents. In: Brka, M., et al. 32nd Scientific-Expert Conference of Agriculture and Food Industry. Agriconference 2022. Lecture Notes in Bioengineering. Springer, Cham. https://doi.org/10.1007/978-3-031-47467-5_20
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