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3D Cell Culture Techniques

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Animal Cell Culture: Principles and Practice

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Abstract

To better understand human physiology and its development and pathological conditions, in vitro cell culture models are recognised as effective research tools. So far, traditional 2D cell culture methods have been used extensively. However, unfortunately, it lacks the crucial architecture of native cells and tissues, failing complete information about biological processes in vivo. Therefore, three-dimensional (3D) cell culture had come into the limelight and emerged as an advanced culture system that fills the barrier between two-dimensional cell culture models and animal modeling. It mimics the characteristic features of in vivo environment of animal physiology like cellular heterogeneity, structure and functions of cells, which offers a novel perspective on the behaviour of stem cells, growth of tissues and organs and pathological conditions including cancers. The 3D cell culture models might help promote translational research in diseased models, drug discovery, tissue engineering and personalised medicine development.

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Authors and Affiliations

  1. Genome Biology Laboratory, Department of Biosciences, Jamia Millia Islamia, New Delhi, India

    Madhu Rani & Annu Devi

  2. Special Centre of Molecular Medicine, Jawaharlal Nehru University, New Delhi, India

    Shashi Prakash Singh & Rashmi Kumari

  3. Rani Lakshmi Bai Central Agricultural University, Jhansi, Uttar Pradesh, India

    Anil Kumar

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  1. Madhu Rani
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  2. Annu Devi
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  3. Shashi Prakash Singh
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  4. Rashmi Kumari
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  5. Anil Kumar
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Rani, M., Devi, A., Singh, S.P., Kumari, R., Kumar, A. (2023). 3D Cell Culture Techniques. In: Animal Cell Culture: Principles and Practice. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-031-19485-6_14

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