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Evaluation of the Formation of an Ionic-Complementary Self-assembling Peptide Hydrogel for the Three-Dimensional Culture of Mammalian Cells in Vitro

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XLV Mexican Conference on Biomedical Engineering (CNIB 2022)

Part of the book series: IFMBE Proceedings ((IFMBE,volume 86))

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Abstract

Standard treatments for chronic-diseases face limitations related to drug schemes difficult to accomplish by patients, as well as poor drug effectivity. Self-assembling peptide hydrogels made from amino acids, form secondary structures, which ultimately form nanofibril networks that mimic the architecture of the extracellular matrix for three-dimensional (3D) cell culture, drug delivery, and regenerative medicine applications. The aims of this study are evaluating the self-assembly of the nano-peptide FEFEFKFKK (F9) to form a hydrogel and the capability of such a hydrogel to support the 3D culture of human cells in vitro. The gelation of F9 was investigated using the tilt tube test. Cell viability in the F9 hydrogel, was monitored using lived/dead assay and confocal microscopy. The sol-gel transition of the peptide F9 was held at pH 5 after the progressive addition of salts, where a self-supported and transparent gel was formed at room temperature. Human cells were viable and distributed throughout the F9 gel at day 5 of 3D culture. The outcomes reported here, suggest that the F9 hydrogel is a promising alternative to act as a 3D scaffolding for cell culture and regenerative medicine applications.

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Authors and Affiliations

  1. Laboratorio de Biotecnología Odontológica, Departamento de Medicina y Ciencias de la Salud, Universidad de Sonora, Luis Donaldo Colosio, S/N, Colonia Centro, Hermosillo, Sonora, Mexico

    Brandhon Francisco Flores-Ibarra & Luis Alberto Castillo-Díaz

Authors
  1. Brandhon Francisco Flores-Ibarra
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  2. Luis Alberto Castillo-Díaz
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Corresponding author

Correspondence to Luis Alberto Castillo-Díaz .

Editor information

Editors and Affiliations

  1. Instituto Nacional de Rehabilitación-LGII, Mexico, Mexico

    Citlalli Jessica Trujillo-Romero

  2. Departamento de Ingeniería Eléctrica y Computación, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Mexico

    Rafael Gonzalez-Landaeta

  3. Instituto de Ingeniería y Tecnología, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Mexico

    Christian Chapa-González

  4. Facultad de Ciencias Campus Pedregal, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico

    Guadalupe Dorantes-Méndez

  5. Universidad Autónoma de Baja California, Ensenada, Mexico

    Dora-Luz Flores

  6. Conacyt-Instituto Tecnológico de Orizaba, Orizaba, Mexico

    J. J. Agustin Flores Cuautle

  7. Departamento de Ingeniería Eléctrica, Universidad Autónoma Metropolitana Iztapalapa, Mexico, Mexico

    Martha R. Ortiz-Posadas

  8. Departamento de Bioingeniería Traslacional, Universidad de Guadalajara, Guadalajara, Mexico

    Ricardo A. Salido Ruiz

  9. Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Chihuahua, Mexico

    Esmeralda Zuñiga-Aguilar

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Flores-Ibarra, B.F., Castillo-Díaz, L.A. (2023). Evaluation of the Formation of an Ionic-Complementary Self-assembling Peptide Hydrogel for the Three-Dimensional Culture of Mammalian Cells in Vitro. In: Trujillo-Romero, C.J., et al. XLV Mexican Conference on Biomedical Engineering. CNIB 2022. IFMBE Proceedings, vol 86. Springer, Cham. https://doi.org/10.1007/978-3-031-18256-3_76

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  • DOI: https://doi.org/10.1007/978-3-031-18256-3_76

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-031-18255-6

  • Online ISBN: 978-3-031-18256-3

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