Abstract
Cerebellar Purkinje neurons communicate with downstream circuit elements by generating two distinct types of electrical activity. Purkinje neurons fire conventional action potentials, termed simple spikes, and they also intermittently fire a highly stereotyped burst of decrementing spikes, called a complex spike. Each of these types of electrical activity arises from an interaction between synaptic input and distinct excitability mechanisms intrinsic to Purkinje neurons. Simple spikes occur at very high frequencies in the range of 50 spikes per second and are driven by pacemaking ion channels expressed by Purkinje neurons. This high simple spike rate is then modulated by excitatory and inhibitory synaptic input. Complex spikes occur in response to excitatory synaptic input from the climbing fiber; these compound electrical events are driven in part by the large voltage-gated calcium conductance in the dendrites of Purkinje neurons. Finally, the two forms of excitability interact; complex spikes can exert indirect effects on simple spike firing rates. Together, these two firing modes endow Purkinje neurons with a range of signaling behaviors critical for cerebellar contributions to motor coordination and motor learning.
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References
Barmack NH, Yakhnitsa V (2011) Topsy turvy: functions of climbing and mossy fibers in the vestibulo-cerebellum. Neuroscientist 17:221–236
Bell CC, Grimm RJ (1969) Discharge properties of Purkinje cells recorded on single and double microelectrodes. J Neurophysiol 32:1044–1055
Cerminara NL, Rawson JA (2004) Evidence that climbing fibers control an intrinsic spike generator in cerebellar Purkinje cells. J Neurosci 24:4510–4517
Cook AA, Fields E, Watt AJ (2021) Losing the beat: contribution of Purkinje cell firing dysfunction to disease, and its reversal. Neurosci 462:247–261
Davie JT, Clark BA, Hausser M (2008) The origin of the complex spike in cerebellar Purkinje cells. J Neurosci 28:7599–7609
Eccles J, Llinas R, Sasaki K (1964) Excitation of cerebellar Purkinje cells by the climbing fibres. Nature 203:245–246
Grieco TM, Malhotra JD, Chen C, Isom LL, Raman IM (2005) Open-channel block by the cytoplasmic tail of sodium channel beta4 as a mechanism for resurgent sodium current. Neuron 45:233–244
Hansen ST, Meera P, Otis TS, Pulst SM (2013) Changes in Purkinje cell firing and gene expression precede behavioral pathology in a mouse model of SCA2. Hum Mol Genet 22:271–283
Hausser M, Clark BA (1997) Tonic synaptic inhibition modulates neuronal output pattern and spatiotemporal synaptic integration. Neuron 19:665–678
Hourez R, Servais L, Orduz D, Gall D, Millard I, de Kerchove d’E A, Cheron G, Orr HT, Pandolfo M, Schiffmann SN (2011) Aminopyridines correct early dysfunction and delay neurodegeneration in a mouse model of spinocerebellar ataxia type 1. J Neurosci 31:11795–11807
Kitamura K, Hausser M (2011) Dendritic calcium signaling triggered by spontaneous and sensory-evoked climbing fiber input to cerebellar Purkinje cells in vivo. J Neurosci 31:10847–10858
Martina M, Metz AE, Bean BP (2007) Voltage-dependent potassium currents during fast spikes of rat cerebellar Purkinje neurons: inhibition by BDS-I toxin. J Neurophysiol 97:563–571
Mathews PJ, Lee KH, Peng Z, Houser CR, Otis TS (2012) Effects of climbing fiber driven inhibition on Purkinje neuron spiking. J Neurosci 32:17988–17997
Mauk MD, Steinmetz JE, Thompson RF (1986) Classical conditioning using stimulation of the inferior olive as the unconditioned stimulus. Proc Natl Acad Sci U S A 83:5349–5353
Medina JF, Lisberger SG (2008) Links from complex spikes to local plasticity and motor learning in the cerebellum of awake-behaving monkeys. Nat Neurosci 11:1185–1192
Otis TS, Mathews PJ, Lee KH, Maiz J (2012) How do climbing fibers teach? Front Neural Circ 6:95
Raman IM, Bean BP (1999) Ionic currents underlying spontaneous action potentials in isolated cerebellar Purkinje neurons. J Neurosci 19:1663–1674
Raman IM, Sprunger LK, Meisler MH, Bean BP (1997) Altered subthreshold sodium currents and disrupted firing patterns in Purkinje neurons of Scn8a mutant mice. Neuron 19:881–891
Raymond JL, Lisberger SG, Mauk MD (1996) The cerebellum: a neuronal learning machine? Science 272:1126–1131
Shakkottai VG, do Carmo Costa M, Dell’Orco JM, Sankaranarayanan A, Wulff H, Paulson HL (2011) Early changes in cerebellar physiology accompany motor dysfunction in the polyglutamine disease spinocerebellar ataxia type 3. J Neurosci 31:13002–13014
Swensen AM, Bean BP (2003) Ionic mechanisms of burst firing in dissociated Purkinje neurons. J Neurosci 23:9650–9663
Tank DW, Sugimori M, Connor JA, Llinas RR (1988) Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice. Science 242:773–777
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Otis, T.S. (2023). Simple Spikes and Complex Spikes. In: Gruol, D.L., Koibuchi, N., Manto, M., Molinari, M., Schmahmann, J.D., Shen, Y. (eds) Essentials of Cerebellum and Cerebellar Disorders. Springer, Cham. https://doi.org/10.1007/978-3-031-15070-8_40
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DOI: https://doi.org/10.1007/978-3-031-15070-8_40
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