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Preparation of Messenger RNA-Loaded Nanomedicine Applied on Tissue Engineering and Regenerative Medicine

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Messenger RNA Therapeutics

Part of the book series: RNA Technologies ((RNATECHN,volume 13))

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Abstract

Tissue engineering holds great promise for regenerative medicine; the multipotent cell lineage, biocompatible tissue-compliant biomaterials, and critical differentiation cues compose the critical factors for successful tissue engineering applied to regenerative medicine. The critical differentiation cues could be provided by recombinant proteins, alternative novel therapeutics, and gene therapy, including viral and non-viral-based methodology. One of the emerged gene therapeutics is messenger RNA (mRNA) administration, which holds critical advantages, such as tentative drug expression, no DNA backbone remaining in the cytosol, and easy to be manufactured as cocktail therapeutics and scaled up in a good manufacture process (GMP) factory. However, the successful mRNA medicine applied to tissue engineering remains challenging, including sophisticated mRNA medicine carriers, endosomal escape capability, targeted delivery, fine-tuned gene expression duration, and subsequent immune responses. This chapter will discuss the up-to-date technologies addressed on mRNA manufacture, sophisticated carrier design, mRNA medicine endosomal escape, nuclease resistance, and sustained expression. Furthermore, combined with tissue engineering, we will also introduce the mRNA medicine selection for therapeutic purposes, applied on the diseased animal model, mRNA activated matrix construction, and subsequent evaluation criteria will also be addressed.

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Acknowledgements

The authors thank Ms. Zhu-Yin Chen and the Medical Research Core Facilities, Office of Research & Development at China Medical University, Taichung, Taiwan, R.O.C., for their assistance in experiments and data analysis.

Grant Support

This work was financially supported by the Minister of Science and Technology (MOST 108–2221-E-039–006-MY3, MOST 107–2314-B-039–023, MOST 107–2622-E-039–003-CC1 and MOST 108–2622-E-039–002-CC1) and China Medical University (CMU110-MF-83). However, the funding sources had no involvement in study design, the collection, analysis, and interpretation of data, the report’s writing, and the decision to submit the article for publication.

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Authors and Affiliations

  1. Institute of New Drug Development, College of Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan

    Hsi-Kai Tsou, Cheng-Chung Chang & Chin-Yu Lin

  2. Functional Neurosurgery Division, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan

    Hsi-Kai Tsou

  3. Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung, Taiwan

    Tomoji Maeda & Chin-Yu Lin

  4. Department of Pharmaceutical Sciences, Nihon Pharmaceutical University, Saitama, Japan

    Tomoji Maeda

  5. Department of Rehabilitation, Jen-Teh Junior College of Medicine, Miaoli County, Taiwan

    Hsi-Kai Tsou

Authors
  1. Hsi-Kai Tsou
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  2. Cheng-Chung Chang
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  3. Tomoji Maeda
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  4. Chin-Yu Lin
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Corresponding author

Correspondence to Chin-Yu Lin .

Editor information

Editors and Affiliations

  1. NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

    Stefan Jurga

  2. NanoBioMedical Center, Adam Mickiewicz University, Poznań, Poland

    Jan Barciszewski

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Tsou, HK., Chang, CC., Maeda, T., Lin, CY. (2022). Preparation of Messenger RNA-Loaded Nanomedicine Applied on Tissue Engineering and Regenerative Medicine. In: Jurga, S., Barciszewski, J. (eds) Messenger RNA Therapeutics. RNA Technologies, vol 13. Springer, Cham. https://doi.org/10.1007/978-3-031-08415-7_18

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