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Current Trends and Prospects in Antimicrobial Peptide Bioprocessing

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Microbial Production of High-Value Products

Part of the book series: Microbiology Monographs ((MICROMONO,volume 37))

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Abstract

The increase in resistance to conventional antimicrobials in recent years has boosted the search for new antibiotics to treat serious infectious diseases, especially those generated by multi-resistant bacteria. In this context, antimicrobial peptides (AMPs) are alternative molecules for use as new therapeutic agents. AMPs are small bioactive proteins commonly produced by all living organisms, and they can be part of innate immunity. Due to their broad-spectrum antibacterial potential and other activities, including immunomodulatory and antitumor, they are of great interest to the pharmaceutical industry’s production of biopharmaceuticals. Among the technological platforms applied in the process of development and manufacturing of AMPs, recombinant DNA technology has enabled the production of such molecules using bacterial and yeast cells as expression host systems on a laboratory scale and in large-scale environments. Furthermore, different bioprocessing strategies can be used for peptide industrial production, aiming to optimize the yield, make cultures more robust and significantly increase cell density. In this chapter, we will address recent developments and future directions in AMPs bioprocessing, including microbial expression systems, as well as bioprocessing and purification technologies. Here we also describe successful cases in this field and emphasize the prospects and challenges related to AMPs bioengineering.

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Acknowledgments

This work was supported by the Coordination of Improvement of Higher Education Personnel (CAPES); National Council of Technological and Scientific Development (CNPq), Federal District Research Support Foundation (FAPDF) and Support Foundation for the Development of Education, Science and Technology of the State of Mato Grosso do Sul (FUNDECT).

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Authors and Affiliations

  1. Centro de Análises Proteômicas e Bioquímicas, Pós-graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Brazil

    Kamila Botelho Sampaio de Oliveira, Michel Lopes Leite, Gisele Regina Rodrigues, Nicolau Brito da Cunha, Simoni Campos Dias & Octavio Luiz Franco

  2. Departamento de Biologia Molecular, Instituto de Ciências Biológicas, Campus Darcy Ribeiro, Universidade de Brasília, Brasília, Distrito Federal, Brazil

    Michel Lopes Leite

  3. Universidade de Brasília, Pós-graduação em Biologia Animal, Campus Darcy Ribeiro, Brasília, Brazil

    Simoni Campos Dias

  4. Universidade de Brasília, Pós-graduação em Patologia Molecular, Campus Darcy Ribeiro, Brasília, Brazil

    Octavio Luiz Franco

  5. S-Inova Biotech, Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil

    Octavio Luiz Franco

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  1. Kamila Botelho Sampaio de Oliveira
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  4. Nicolau Brito da Cunha
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Editors and Affiliations

  1. Centre for Cell Factories and Biopolymers, Griffith Institute for Drug Discovery, Menzies Health Institute Queensland, Griffith University, Brisbane, QLD, Australia

    Bernd H. A. Rehm

  2. Centre for Cell Factories and Biopolymers, Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD, Australia

    David Wibowo

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de Oliveira, K.B.S., Leite, M.L., Rodrigues, G.R., da Cunha, N.B., Dias, S.C., Franco, O.L. (2022). Current Trends and Prospects in Antimicrobial Peptide Bioprocessing. In: Rehm, B.H.A., Wibowo, D. (eds) Microbial Production of High-Value Products. Microbiology Monographs, vol 37. Springer, Cham. https://doi.org/10.1007/978-3-031-06600-9_5

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