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Laboratory Protocols in Medical Biotechnology II (Contemporary Principles and Practices of Bacterial and Human Cell Culture)

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Fundamentals and Advances in Medical Biotechnology

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Abstract

Cell culture is a technique in which eukaryotic cells are grown in the laboratory under controlled physiological conditions with complex growth requirements, whereas microbial culture is the method of growing microorganisms under optimal conditions with simple growth requirements. Both culture techniques address the basic scientific and translational research queries posed by certain scientific beliefs. Owing to the importance of cell and microbial culture in research and development, it is imperative to carry out these techniques with the highest competence and purity so that the reproducibility and homogeneity of the results are maintained. This chapter elucidates the basic principles and techniques of microbial and cell culture. Purification techniques, media formulation, growth requirement of cells, and decontamination measures are thoroughly discussed. Since the outcome of the research needs to be analyzed and interpreted to reach a convincing conclusion, the basic statistical overview and the methods involved in the analysis of research data are also described herein.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Panjab University, Chandigarh, India

    Shabeer Ahmad Rather & Swati Sharma

  2. Department of Zoology, Panjab University, Chandigarh, India

    Naveed Pervaiz Bhat

  3. Department of Biotechnology, College of Natural and Computational Science, Wachemo University, Hossana, Ethiopia

    Riyaz Ahmad Rather

Authors
  1. Shabeer Ahmad Rather
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  2. Swati Sharma
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  3. Naveed Pervaiz Bhat
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  4. Riyaz Ahmad Rather
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Editor information

Editors and Affiliations

  1. Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, IL, USA

    Mumtaz Anwar

  2. Department of Biotechnology, Wachemo University, Hosaena, Ethiopia

    Riyaz Ahmad Rather

  3. Pharmacology and Regenerative Medicine, University of Illinois at Chicago, Chicago, IL, USA

    Zeenat Farooq

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Rather, S.A., Sharma, S., Bhat, N.P., Rather, R.A. (2022). Laboratory Protocols in Medical Biotechnology II (Contemporary Principles and Practices of Bacterial and Human Cell Culture). In: Anwar, M., Ahmad Rather, R., Farooq, Z. (eds) Fundamentals and Advances in Medical Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-030-98554-7_13

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