Abstract
Hybrids of intrinsic conducting polymers (CPs) and carbon-based materials are frequently studied for technological application in several areas. This chapter discusses recent applications of these hybrids in bioelectrochemistry, with a focus on biosensors. These hybrids presented synergic characteristics such as large surface, significant electrical conductivity, and outstanding thermal and mechanical properties improving the analytical responses as selectivity, sensitivity, reproducibility, and response time, of the biosensors. Thus, these hybrids have been extensively explored in the development of non-enzymatic and enzymatic biosensors and used in medical, environmental, food, and pharmaceutical analysis. Biosensors based on these CPs/carbon-based hybrids are simple, specific, and sensitive as well as enable manufacture disposable, and portable miniature device.
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References
Canobre SC, Almeida DAL, Fonseca CP, Neves S (2009) Synthesis and characterization of hybrid composites based on carbon nanotubes. Electrochim Acta 54:6383–6388
Hur J, Park S-H, Bae J (2015) Elaborate chemical sensors based on graphene/conducting polymer hybrids. Curr Org Chem 19:1117–1133
Bourdo SE, Viswanathan T (2005) Graphite/polyaniline (GP) composites: synthesis and characterization. Carbon 43:2983–2988
Wang L, Wang D, Zhu G et al (2011) Thermoelectric properties of conducting polyaniline/graphite composites. Mater Lett 65:1086–1088
Simoes FR, Capparelli Mattoso LH, Pedro Vaz CM (2006) Conducting polymers as sensor materials for the electrochemical detection of pesticides. Sens Lett 4:319–324
Huang J-C (2002) Carbon black filled conducting polymers and polymer blends. Adv Polym Technol 21:299–313
Simoes FR, Bulhoes LOS, Pereira EC (2009) Synthesis and characterization of conducting composites of polyaniline and carbon black with high thermal stability. Polim-Cienc E Tecnol 19:54–57
Lee H-Y, Jung Y, Kim S (2016) Conducting polymer coated graphene oxide electrode for rechargeable lithium-sulfur batteries. J Nanosci Nanotechnol 16:2692–2695
Zhou H, Han G (2016) One-step fabrication of heterogeneous conducting polymers-coated graphene oxide/carbon nanotubes composite films for high-performance supercapacitors. Electrochim Acta 192:448–455
Lei W, Si W, Xu Y et al (2014) Conducting polymer composites with graphene for use in chemical sensors and biosensors. Microchim Acta 181:707–722
Gokoglan TC, Kesik M, Soylemez S et al (2017) Paper based glucose biosensor using graphene modified with a conducting polymer and gold nanoparticles. J Electrochem Soc 164:G59–G64
An HF, Wang XY, Li N et al (2009) Carbon and conducting polymer composites for supercapacitors. Prog Chem 21:1832–1838
Albayati SAR, Kashanian S, Nazari M, Rezaei S (2019) Novel fabrication of a laccase biosensor to detect phenolic compounds using a carboxylated multiwalled carbon nanotube on the electropolymerized support. Bull Mater Sci 42:187
Yang Y, Zhang L, Li S et al (2013) Electrochemical performance of conducting polymer and its nanocomposites prepared by chemical vapor phase polymerization method. J Mater Sci-Mater Electron 24:2245–2253
Branzoi V, Branzoi F, Pilan L, Donisan N (2010) The characterization of some nanocomposites based on conducting polymers and carbon nanotubes obtained by co-polymerization. Rev Roum Chim 55:369
Frackowiak E, Khomenko V, Jurewicz K et al (2006) Supercapacitors based on conducting polymers/nanotubes composites. J Power Sources 153:413–418
Mottaghitalab V (2005) The influence of carbon nanotubes on mechanical and electrical properties of polyaniline fibers. Synth Met 152:77–80
Alqarni SA, Hussein MA, Ganash AA, Khan A (2020) Composite material-based conducting polymers for electrochemical sensor applications: a mini review. Bionanoscience 10:351–364
Jain R, Jadon N, Pawaiya A (2017) Polypyrrole based next generation electrochemical sensors and biosensors: a review. Trac-Trends Anal Chem 97:363–373
Mohajeri S, Dolati A, Yazdanbakhsh K (2019) Synthesis and characterization of a novel non-enzymatic glucose biosensor based on polyaniline/zinc oxide/multi-walled carbon nanotube ternary nanocomposite. J Electrochem Sci Eng 9:207–222
de Araújo GM, Simões FR (2017) Self-assembled films based on polypyrrole and carbon nanotubes composites for the determination of Diuron pesticide. J Solid State Electrochem
He BL, Dong B, Wang W, Li HL (2009) Performance of polyaniline/multi-walled carbon nanotubes composites as cathode for rechargeable lithium batteries. Mater Chem Phys 114:371–375
Wang Z, Han J-J, Zhang N et al (2019) Synthesis of polyaniline/graphene composite and its application in zinc-rechargeable batteries. J Solid State Electrochem 23:3373–3382
Nemade K, Dudhe P, Tekade P (2018) Enhancement of photovoltaic performance of polyaniline/graphene composite-based dye-sensitized solar cells by adding TiO2 nanoparticles. Solid State Sci 83:99–106
Cogal S, Ali AK, Erten-Ela S et al (2018) Plasma-based preparation of polyaniline/graphene and polypyrrole/graphene composites for dye-sensitized solar cells as counter electrodes. J Macromol Sci Part-Pure Appl Chem 55:317–323
Shin K-Y, Cho S, Jang J (2013) Graphene/polyaniline/poly(4-styrenesulfonate) hybrid film with uniform surface resistance and its flexible dipole tag antenna application. Small 9:3792–3798
Thangamani JG, Deshmukh K, Sadasivuni KK et al (2017) White graphene reinforced polypyrrole and poly(vinyl alcohol) blend nanocomposites as chemiresistive sensors for room temperature detection of liquid petroleum gases. Microchim Acta 184:3977–3987
Qi Z, Ye J, Chen W et al (2018) 3D-printed, superelastic polypyrrole-graphene electrodes with ultrahigh areal capacitance for electrochemical energy storage. Adv Mater Technol 3:1800053
Spinks GM, Xi BB, Truong VT, Wallace GG (2005) Actuation behaviour of layered composites of polyaniline, carbon nanotubes and polypyrrole. Synth Met 151:85–91
Qiu YJ, Yu J, Fang G et al (2009) Synthesis of carbon/carbon core/shell nanotubes with a high specific surface area. J Phys Chem C 113:61–68
Popov A, Aukstakojyte R, Gaidukevic J et al (2021) Reduced graphene oxide and polyaniline nanofibers nanocomposite for the development of an amperometric glucose biosensor. Sensors 21:948
Facure MHM, Mercante LA, Mattoso LHC, Correa DS (2017) Detection of trace levels of organophosphate pesticides using an electronic tongue based on graphene hybrid nanocomposites. Talanta 167:59–66
Cesarino I, Moraes FC, Machado SAS (2011) A biosensor based on polyaniline-carbon nanotube core-shell for electrochemical detection of pesticides. Electroanalysis 23:2586–2593
Fernandez J, Bonastre J, Molina J, Cases F (2018) Electrochemical study on an activated carbon cloth modified by cyclic voltammetry with polypyrrole/anthraquinone sulfonate and reduced graphene oxide as electrode for energy storage. Eur Polym J 103:179–186
Radhapyari K, Kotoky P, Das MR, Khan R (2013) Graphene-polyaniline nanocomposite based biosensor for detection of antimalarial drug artesunate in pharmaceutical formulation and biological fluids. Talanta 111:47–53
Zhuang X, Tian C, Luan F et al (2016) One-step electrochemical fabrication of a nickel oxide nanoparticle/polyaniline nanowire/graphene oxide hybrid on a glassy carbon electrode for use as a non-enzymatic glucose biosensor. Rsc Adv 6:92541–92546
Rahman MM, Shiddiky MJA, Rahman MA, Shim Y-B (2009) A lactate biosensor based on lactate dehydrogenase/nictotinamide adenine dinucleotide (oxidized form) immobilized on a conducting polymer/multiwall carbon nanotube composite film. Anal Biochem 384:159–165
Yang M, Ren X, Yang T et al (2021) Polypyrrole/sulfonated multi-walled carbon nanotubes conductive hydrogel for electrochemical sensing of living cells. Chem Eng J 418:129483
Sethuraman V, Muthuraja P, Raj JA, Manisankar P (2016) A highly sensitive electrochemical biosensor for catechol using conducting polymer reduced graphene oxide-metal oxide enzyme modified electrode. Biosens Bioelectron 84:112–119
Zheng H, Yan Z, Wang M et al (2019) Biosensor based on polyaniline-polyacrylonitrile-graphene hybrid assemblies for the determination of phenolic compounds in water samples. J Hazard Mater 378:120714
Yang Y, Kang M, Fang S et al (2015) Electrochemical biosensor based on three-dimensional reduced graphene oxide and polyaniline nanocomposite for selective detection of mercury ions. Sens Actuators B-Chem 214:63–69
Soylemez S (2019) A conjugated polymer and SWCNTs transducer for an effective biosensing tool. J Electrochem Soc 166:B853–B858
Nia PM, Meng WP, Lorestani F et al (2015) Electrodeposition of copper oxide/polypyrrole/reduced graphene oxide as a nonenzymatic glucose biosensor. Sens Actuators B-Chem 209:100–108
Xu Q, Gu S-X, Jin L et al (2014) Graphene/polyaniline/gold nanoparticles nanocomposite for the direct electron transfer of glucose oxidase and glucose biosensing. Sens Actuators B-Chem 190:562–569
Shrestha BK, Ahmad R, Mousa HM et al (2016) High-performance glucose biosensor based on chitosan-glucose oxidase immobilized polypyrrole/Nafion/functionalized multi-walled carbon nanotubes bio-nanohybrid film. J Colloid Interface Sci 482:39–47
Radhakrishnan S, Sumathi C, Umar A et al (2013) Polypyrrole-poly(3,4-ethylenedioxythiophene)-Ag (PPy-PEDOT-Ag) nanocomposite films for label-free electrochemical DNA sensing. Biosens Bioelectron 47:133–140
Wang L, Hua E, Liang M et al (2014) Graphene sheets, polyaniline and AuNPs based DNA sensor for electrochemical determination of BCR/ABL fusion gene with functional hairpin probe. Biosens Bioelectron 51:201–207
Li Y, Zhang Y, Han G et al (2016) An acetylcholinesterase biosensor based on graphene/polyaniline composite film for detection of pesticides. Chin J Chem 34:82–88
Liu GD, Lin YH (2006) Biosensor based on self-assembling acetylcholinesterase on carbon nanotubes for flow injection/amperometric detection of organophosphate pesticides and nerve agents. Anal Chem 78:835–843
Liu K, Dong H, Deng Y (2016) Recent advances on rapid detection of pesticides based on enzyme biosensor of nanomaterials. J Nanosci Nanotechnol 16:6648–6656
Yang Y, Asiri AM, Du D, Lin Y (2014) Acetylcholinesterase biosensor based on a gold nanoparticle-polypyrrole-reduced graphene oxide nanocomposite modified electrode for the amperometric detection of organophosphorus pesticides. Analyst 139:3055–3060
Min K, Freeman C, Kang H, Choi S-U (2015) The regulation by phenolic compounds of soil organic matter dynamics under a changing environment. Biomed Res Int 2015:825098
Saidur MR, Aziz ARA, Basirun WJ (2017) Recent advances in DNA-based electrochemical biosensors for heavy metal ion detection: a review. Biosens Bioelectron 90:125–139
Cui L, Wu J, Ju H (2015) Electrochemical sensing of heavy metal ions with inorganic, organic and bio-materials. Biosens Bioelectron 63:276–286
Kaur N, Thakur H, Prabhakar N (2016) Conducting polymer and multi-walled carbon nanotubes nanocomposites based amperometric biosensor for detection of organophosphate. J Electroanal Chem 775:121–128
Pundir CS, Chauhan N (2012) Acetylcholinesterase inhibition-based biosensors for pesticide determination: a review. Anal Biochem 429:19–31
Kesik M, Kanik FE, Turan J et al (2014) An acetylcholinesterase biosensor based on a conducting polymer using multiwalled carbon nanotubes for amperometric detection of organophosphorous pesticides. Sens Actuators B-Chem 205:39–49
Cesarino I, Moraes FC, Lanza MRV, Machado SAS (2012) Electrochemical detection of carbamate pesticides in fruit and vegetables with a biosensor based on acetylcholinesterase immobilised on a composite of polyaniline-carbon nanotubes. Food Chem 135:873–879
Viswanathan S, Radecka H, Radecki J (2009) Electrochemical biosensors for food analysis. Monatshefte Chem 140:891–899
Kaur N, Thakur H, Prabhakar N (2019) Multi walled carbon nanotubes embedded conducting polymer based electrochemical aptasensor for estimation of malathion. Microchem J 147:393–402
Lata S, Batra B, Singala N, Pundir CS (2013) Construction of amperometric l-amino acid biosensor based on l-amino acid oxidase immobilized onto ZnONPs/c-MWCNT/PANI/AuE. Sens Actuators B Chem 1088:1080–1088
Lu L, Zhang O, Xu J et al (2013) A facile one-step redox route for the synthesis of graphene/poly (3,4-ethylenedioxythiophene) nanocomposite and their applications in biosensing. Sens Actuators B-Chem 181:567–574
Chen C, Xie Q, Yang D et al (2013) Recent advances in electrochemical glucose biosensors: a review. Rsc Adv 3:4473–4491
Hsu C-W, Su F-C, Peng P-Y et al (2016) Highly sensitive non-enzymatic electrochemical glucose biosensor using a photolithography fabricated micro/nano hybrid structured electrode. Sens Actuators B-Chem 230:559–565
Nambiar S, Yeow JTW (2011) Conductive polymer-based sensors for biomedical applications. Biosens Bioelectron 26:1825–1832
He W, Huang Y, Wu J (2020) Enzyme-free glucose biosensors based on MoS2 nanocomposites. Nanoscale Res Lett 15:60
Sassolas A, Blum LJ, Leca-Bouvier BD (2012) Immobilization strategies to develop enzymatic biosensors. Biotechnol Adv 30:489–511
Xue K, Zhou S, Shi H et al (2014) A novel amperometric glucose biosensor based on ternary gold nanoparticles/polypyrrole/reduced graphene oxide nanocomposite. Sens Actuators B-Chem 203:412–416
Tan XC, Ll MJ, Cai PX et al (2005) An amperometric cholesterol biosensor based on multiwalled carbon nanotubes and organically modified sol-gel/chitosan hybrid composite film. Anal Biochem 337:111–120
Amiri M, Arshi S (2020) An overview on electrochemical determination of cholesterol. Electroanalysis 32:1391–1407
Arya SK, Datta M, Malhotra BD (2008) Recent advances in cholesterol biosensor. Biosens Bioelectron 23:1083–1100
Dey RS, Raj CR (2010) Development of an amperometric cholesterol biosensor based on graphene-Pt nanoparticle hybrid material. J Phys Chem C 114:21427–21433
Ruecha N, Rangkupan R, Rodthongkum N, Chailapakul O (2014) Novel paper-based cholesterol biosensor using graphene/polyvinylpyrrolidone/polyaniline nanocomposite. Biosens Bioelectron 52:13–19
Alexander S, Barapeedharan P, Balasubrahmanyan S, Ramaprabhu S (2017) Modified graphene based molecular imprinted polymer for electrochemical non-enzymatic cholesterol biosensor. Eur Polym J 86:106–116
Gautam V, Singh KP, Yadav VL (2018) Polyaniline/MWCNTs/starch modified carbon paste electrode for non-enzymatic detection of cholesterol: application to real sample (cow milk). Anal Bioanal Chem 410:2173–2181
Chen Y, Guo S, Zhao M et al (2018) Amperometric DNA biosensor for Mycobacterium tuberculosis detection using flower-like carbon nanotubes-polyaniline nanohybrid and enzyme-assisted signal amplification strategy. Biosens Bioelectron 119:215–220
Chen Y, Li Y, Yang Y et al (2017) A polyaniline-reduced graphene oxide nanocomposite as a redox nanoprobe in a voltammetric DNA biosensor for Mycobacterium tuberculosis. Microchim Acta 184:1801–1808
Liu C, Jiang D, Xiang G et al (2014) An electrochemical DNA biosensor for the detection of Mycobacterium tuberculosis, based on signal amplification of graphene and a gold nanoparticle-polyaniline nanocomposite. Analyst 139:5460–5465
Pasinszki T, Krebsz M, Tung TT, Losic D (2017) Carbon nanomaterial based biosensors for non-invasive detection of cancer and disease biomarkers for clinical diagnosis. Sensors 17:1919
Tezerjani MD, Benvidi A, Rezaeinasab M et al (2016) An impedimeric biosensor based on a composite of graphene nanosheets and polyaniline as a suitable platform for prostate cancer sensing. Anal Methods 8:7507–7515
Asadian E, Shahrokhian S, Zad AI, Jokar E (2014) In-situ electro-polymerization of graphene nanoribbon/polyaniline composite film: Application to sensitive electrochemical detection of dobutamine. Sens Actuators B-Chem 196:582–588
Khodadadi A, Faghih-Mirzaei E, Karimi-Maleh H et al (2019) A new epirubicin biosensor based on amplifying DNA interactions with polypyrrole and nitrogen-doped reduced graphene: experimental and docking theoretical investigations. Sens Actuators B-Chem 284:568–574
Arora P, Sindhu A, Dilbaghi N, Chaudhury A (2011) Biosensors as innovative tools for the detection of food borne pathogens. Biosens Bioelectron 28:1–12
Jesionowski T, Zdarta J, Krajewska B (2014) Enzyme immobilization by adsorption: a review. Adsorpt-J Int Adsorpt Soc 20:801–821
Datta S, Christena LR, Rajaram YRS (2013) Enzyme immobilization: an overview on techniques and support materials. 3 Biotech 3:1–9.
Cao LQ, van Langen L, Sheldon RA (2003) Immobilised enzymes: carrier-bound or carrier-free? Curr Opin Biotechnol 14:387–394
Klotzbach TL, Watt M, Ansari Y, Minteer SD (2008) Improving the microenvironment for enzyme immobilization at electrodes by hydrophobically modifying chitosan and Nafion (R) polymers. J Membr Sci 311:81–88
Barbosa O, Ortiz C, Berenguer-Murcia A et al (2014) Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization. Rsc Adv 4:1583–1600
Shahrokhian S, Salimian R (2018) Ultrasensitive detection of cancer biomarkers using conducting polymer/electrochemically reduced graphene oxide-based biosensor: application toward BRCA1 sensing. Sens Actuators B-Chem 266:160–169
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The authors thanks FAPESP (2021/08041-4 and 2017/24742-7) CAPES and CNPQ.
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Simões, F.R., de Araújo, G.M., Cardoso, M.A. (2022). Hybrids of Conducting Polymers and Carbon-Based Materials Aiming Biosensors Applications. In: Crespilho, F.N. (eds) Advances in Bioelectrochemistry Volume 3. Springer, Cham. https://doi.org/10.1007/978-3-030-97921-8_6
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