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In Silico Characterization and Structural Modeling of Proteins Involved in Arsenic Tolerance of Hyper Accumulating Fern Pteris Vittata

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Advances in Chemical, Bio and Environmental Engineering (CHEMBIOEN 2021)

Part of the book series: Environmental Science and Engineering ((ESE))

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Abstract

Arsenic is known as the king of poisons due to its toxic nature and is used as pesticide and fertilizers. Accumulation of arsenic in plants causes toxic reactions, negative impact on growth and productivity. Increment in arsenic uptake is directly proportional to oxidative stress resulting in the production of reactive oxygen species. However, few plants have developed mechanism for tolerance by expressing genes or proteins such as antioxidants enzymes, arsenite oxidase, that playing some beneficial role in detoxification against arsenic effect. Pteris vittata is one such example of plants which can tolerate both forms of Arsenic i.e., arsenate (+5) and arsenite (+3). Tolerance to arsenic in P. vittata is due to the activity of three genes i.e., Glyceraledhyde 3-phosphate dehydrogenase C1 (PvGAPC1), Organic cation/carnitine transporter 4 (PvOCT4), and Glutathione S transferase (PvGSTF1). Knowing the importance of these genes in arsenic tolerance, in the present investigation we have characterized and predicted the tertiary structure of these proteins using computational approaches. Further to understand the mechanism of arsenic tolerance, we have compared the 3D structure of PvGAPC1, PvOCT4 and PvGSTF1 with its counterpart predicted from arsenic sensitive Oryza sativa and Arabidopsis thaliana. Thus, crop improvement techniques using these genes of Pteris vittata be utilized for developing arsenic tolerance in important cultivated crops.

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Acknowledgements

Authors like to thank GGS Indraprastha University, New Delhi and IIT Kharagpur, for all the support and encouragement.

Competing Interests

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Agricultural and Food Engineering, IIT Kharagpur, Kharagpur, West Bengal, 721302, India

    Rahul Deogam

  2. University School of Biotechnology, Guru Gobind Singh Indraprastha University, Sec-16C, Dwarka, New Delhi, 110078, India

    Nikhil Kumar Pipil, Navjyoti Chakraborty, Sayan Chatterjee & Ram Singh Purty

Authors
  1. Rahul Deogam
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  2. Nikhil Kumar Pipil
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  3. Navjyoti Chakraborty
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  4. Sayan Chatterjee
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  5. Ram Singh Purty
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Corresponding author

Correspondence to Ram Singh Purty .

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Editors and Affiliations

  1. Department of Chemical Engineering, Dr. B. R. Ambedkar National Institute of Technology Jalandhar, Jalandhar, Punjab, India

    Jatinder Kumar Ratan

  2. Department of Chemical Engineering, Dr. B. R. Ambedkar National Institute of Technology Jalandhar, Jalandhar, Punjab, India

    Deepak Sahu

  3. Department of Chemical Engineering, Dr. B. R. Ambedkar National Institute of Technology Jalandhar, Jalandhar, Punjab, India

    Nitin Naresh Pandhare

  4. Department of Chemical Engineering, Dr. B. R. Ambedkar National Institute of Technology Jalandhar, Jalandhar, Punjab, India

    Anjireddy Bhavanam

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Deogam, R., Pipil, N.K., Chakraborty, N., Chatterjee, S., Purty, R.S. (2022). In Silico Characterization and Structural Modeling of Proteins Involved in Arsenic Tolerance of Hyper Accumulating Fern Pteris Vittata. In: Ratan, J.K., Sahu, D., Pandhare, N.N., Bhavanam, A. (eds) Advances in Chemical, Bio and Environmental Engineering. CHEMBIOEN 2021. Environmental Science and Engineering. Springer, Cham. https://doi.org/10.1007/978-3-030-96554-9_28

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