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Introduction to Genome Editing in Induced Pluripotent Stem Cells, Gametes, and Embryos

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Regulation of Genome Editing in Human iPS Cells

Abstract

This chapter discusses recent findings and technological advances in stem cell biology, in gametogenesis, in genetic engineering and in the potential treatment of hereditary diseases by means of gene and cell therapy-approaches. Important scientific mile stones for human germline interventions include the understanding of reprogramming approaches that drive terminally differentiated somatic cells into pluripotent stem cells, exhibiting all major characteristics of embryonic stem cells. Furthermore, recent advances in the derivation and differentiation of pluripotent stem cells paved the way for a clinical application of such derivatives and ask for the possibility to generate either female and/or male gametes from such cells for reproductive purposes. Also, recent advances in genetic engineering, such as the CRISPR/Cas technology, fuelled the discussion about safety and efficacy of germ-line interventions in the foreseeable future. Finally, two scenarios are discussed to challenge the current normative view on targeted genome interventions during in vitro fertilization applications for couples suffering from hereditary diseases.

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Notes

  1. 1.

    Gurdon (1962).

  2. 2.

    Wilmut et al. (1997).

  3. 3.

    Tachibana et al. (2013); Johannesson et al. (2014).

  4. 4.

    Takahashi and Yamanaka (2006).

  5. 5.

    Okita et al. (2007).

  6. 6.

    Eakin et al. (2005).

  7. 7.

    Kang et al. (2009); Zhao et al. (2009).

  8. 8.

    Stadtfeld et al. (2010).

  9. 9.

    Carey et al. (2011).

  10. 10.

    Wu et al. (2011).

  11. 11.

    Blau and Daley (2019).

  12. 12.

    Blau and Daley (2019).

  13. 13.

    Jinek et al. (2012).

  14. 14.

    Stadtmauer et al. (2020).

  15. 15.

    Zhu et al. (2020).

  16. 16.

    Saitou and Miyauchi (2016).

  17. 17.

    Nayernia et al. (2006).

  18. 18.

    Nayernia et al. (2009).

  19. 19.

    Mulder et al. (2016).

  20. 20.

    Advena-Regnery et al. (2018).

  21. 21.

    Cornu et al. (2017).

  22. 22.

    Mulder et al. (2016).

  23. 23.

    Griesenbach et al. (2015); Prakash et al. (2016).

  24. 24.

    Cutting (2015).

  25. 25.

    AAVS1: adeno-associated virus integration site 1 locus in the first intron of PPP1R12C gene; The AAVS1-locus is a well-established safe-harbour genomic region that allows the expression of delivered DNA sequences without relevant adverse effects for the host organism.

  26. 26.

    Sadelain et al. (2011).

  27. 27.

    Cre recombinase: Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites.

  28. 28.

    Abremski and Hoess (1984).

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Authors and Affiliations

  1. Translational Hepatology and Stem Cell Biology, Department of Gastroenterology, Hepatology and Endocrinology, REBIRTH Research Center for Translational Regenerative Medicine, Hannover Medical School, Hannover, Germany

    Tobias Cantz

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  1. Tobias Cantz
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Correspondence to Tobias Cantz .

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Editors and Affiliations

  1. Faculty of Law, University of Passau, Passau, Germany

    Hans-Georg Dederer

  2. Faculty of Law, University of Passau, Passau, Germany

    Gregor Frenken

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Cantz, T. (2022). Introduction to Genome Editing in Induced Pluripotent Stem Cells, Gametes, and Embryos. In: Dederer, HG., Frenken, G. (eds) Regulation of Genome Editing in Human iPS Cells. Springer, Cham. https://doi.org/10.1007/978-3-030-93023-3_2

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  • DOI: https://doi.org/10.1007/978-3-030-93023-3_2

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