Abstract
Upconversion nanoparticles (UCNPs) are excited in the near infrared (NIR) biological transparency window, but emits anti-Stokes shifted photons in the visible that are extremely photostable and ideally suited for biomedical imaging. First, we synthesize a UCNP-mOrange fluorescent protein conjugate that forms a Forster resonance energy transfer (FRET) pair with UCNP as the donor and mOrange as the acceptor to report intracellular pH by fluorescence imaging. The inactivity of the mOrange protein in acidic pH will decrease FRET efficiency making the emission green dominated by the UCNPs. In basic pH, the emission will be relatively orange. This is the first demonstration of ratiometric and self-ratiometric demonstration of intracellular pH imaging by the UCNPs. Next, we show a smart multifunctional nanoformulation of the UCNPs (NaGdF4: Yb, Er- 99mTc) using radioisotope labelling that shows potential for multimodal imaging using fluorescence, CT, and SPECT modalities. Intricate chemistry to engineer the UCNPs resulted in stabilizing the nanoformulation in physiological and aqueous environments with reproducible signal acquisition. This work gathers the expertise of physicists, chemists, and biologists in an interdisciplinary platform.
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References
Ashok AH, Mizuno Y, Howes OD (2019) Tobacco smoking and dopaminergic function in humans: a meta-analysis of molecular imaging studies. Psychopharmacology 236(4):1119–1129
Gupta A, Lam CW, Wu CT, Yang DM, Chattopadhyay S (2018) Photothermal disintegration of 3T3 derived fat droplets by irradiated silica coated upconversion nanoparticles. Part Part Syst Charact 35:1800294
Bi H, He F, Dai Y, Xu J, Dong Y et al (2018) Quad-model imaging- guided high-efficiency phototherapy based on upconversion nanoparticles and ZnFe2O4 integrated graphene oxide. Inorg Chem 57(16):9988–9998
Ghosh S, Chiang WC, Fakhri MY, Wu CT, Chen RS, Chattopadhyay S (2020) Ultrasensitive broadband photodetector using electrostatically conjugated MoS2-upconversion nanoparticle nanocomposite. Nano Energy 67:104258
Cao T, Yang T, Gao Y, Yang Y, Hu H, Li F (2010) Water-soluble NaYF4:Yb/Er upconversion nanophosphors: synthesis, characteristics and application in bioimaging. Inorg Chem Commun 13:392–394
Dennis AM, Rhee WJ, Sotto D, Dublin SN, Bao G (2012) Quantum dot-fluorescent protein FRET probes for sensing intracellular pH. ACS Nano 6:2917–2924
Ghosh S, Chang YF, Yang DM, Chattopadhyay S (2020) Upconversion nanoparticle-mOrange protein FRET nanoprobes for self-ratiometric/ratiometric determination of intracellular pH, and single cell pH imaging. Bios Bioelectron 155:112115
Acknowledgements
The authors would like to thank Ministry of Science and Technology, Taiwan (MOST-107-2112-M-010-003-MY3) for the research funding.
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Chattopadhyay, S., Ghosh, S., Akhtar, N., Yang, DM., Chen, C.L., Liu, RS. (2022). Upconversion Nanoformulation for Functional Imaging. In: Lin, KP., Liu, RS., Yang, BH. (eds) Future Trends and Challenges of Molecular Imaging and AI Innovation. FASMI 2020. Springer Proceedings in Physics, vol 272. Springer, Cham. https://doi.org/10.1007/978-3-030-92786-8_5
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DOI: https://doi.org/10.1007/978-3-030-92786-8_5
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