Abstract
Nodule development starts with dedifferentiation of the already differentiated root tissues. Dedifferentiation involves a complex but finely tuned, coherent host, and symbiont crosstalk that ultimately leads to the development of an extraordinary new organ called “root nodule.” This developmental process is regulated by innumerable interconnected transcriptional networks. Medicago generates a cylindrical-shaped indeterminant nodule where a developmental gradient persists from the nodule tip to the base. Both plant and symbiont undergo a huge transcriptomic change as they adjusted to the mutualistic lifestyle. Transcriptome analysis on nodule developmental time series, mutant nodules, laser-capture microdissection (LCM) of separate nodule zones, and single-cell transcriptomics of root hair cells allowed researchers to understand the complex developmental circuit during nodule development at a spatio-temporal resolution. In this chapter, we will focus on the advancement in the transcriptomics study that leads to the understanding of Medicago nodule development in finer details.
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We thank NIPGR for core grant and Department of Biotechnology (DBT)-eLibrary Consortium (DeLCON), India for providing access to e-resources. Akanksha Bhardwaj was supported by CSIR (09/803(0145)/2018-EMR-I).
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Bhardwaj, A., Sinharoy, S. (2022). Understanding of Root Nodule Development at Level of System Biology as Obtained by High Throughput Transcriptomic Approach. In: Sinharoy, S., Kang, Y., Benedito, V. (eds) The Medicago truncatula Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-90757-0_7
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