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Agrobacterium Tumefaciens-Mediated Genetic Transformation in Cucumber

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The Cucumber Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

Genetic transformation is a versatile platform that is playing increasingly important roles in functional genomics studies and crop improvement. Cucumber (Cucumis sativus L.) is an economically important vegetable crop, and a model of choice to understand several biological processes. Although many genetic transformation studies have been carried out in the last four decades, the transformation efficiency and reproducibility of cucumber transformation remain low limiting its widespread and routine use. In this chapter, we reviewed the work in cucumber on Agrobacterium tumefaciens-mediated genetic transformation, and discussed factors affecting its efficiency in each step including genotypes, explant types, the time, temperature and bacterial concentration for co-cultivation, as well as growth regulators and selective agents for transgenic plant regeneration. We emphasized the importance to develop an efficient, reliable and reproducible cucumber genetic transformation system through collaborative and community efforts.

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Acknowledgements

This wok was supported by the AFRI Competitive Grants under award numbers 2015-51181-24285 and 2017-67013-26195 from the USDS-NIFA. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Horticulture Department, University of Wisconsin Madison, Madison, WI, 53706, USA

    Hanqiang Liu & Yiqun Weng

  2. USDA-ARS Vegetable Crops Research Unit, Madison, WI, 53706, USA

    Yiqun Weng

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  1. Hanqiang Liu
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Correspondence to Yiqun Weng .

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Editors and Affiliations

  1. Indian Institute of Vegetable Research, Indian Council of Agricultural Research, Varanasi, Uttar Pradesh, India

    Sudhakar Pandey

  2. USDA-ARS, Horticulture Department, University of Wisconsin, Madison, WI, USA

    Yiqun Weng

  3. Indian Institute of Vegetable Research, Indian Council of Agricultural Research, Varanasi, Uttar Pradesh, India

    Tusar Kanti Behera

  4. The Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China

    Kailiang Bo

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Liu, H., Weng, Y. (2022). Agrobacterium Tumefaciens-Mediated Genetic Transformation in Cucumber. In: Pandey, S., Weng, Y., Behera, T.K., Bo, K. (eds) The Cucumber Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-88647-9_5

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