Skip to main content

Measurement of Gustation: From Clinical to Population-Based Methods

  • Chapter
  • First Online:
Sensory Science and Chronic Diseases

Abstract

Valid measurement of taste is critical for advanced understanding of how variation in taste perception and alterations in taste perception influence dietary behaviors, health, and well-being. Psychophysical procedures exist for measuring the concentration of taste required for detection or recognition, but changes in perceived intensity and liking (hedonic responses) with changes in concentration above threshold are more relevant for diet and health. Here, measures of taste perceived with the whole mouth as well as regional measures of specific taste-related cranial nerve innervation are reviewed. Recommendations and best practices are provided to avoid common mistakes in measuring perceived intensity toward increased ability to understand and document variation in taste and oral sensation. Taste testing is covered for laboratory settings as well as for clinical settings, and for community- and population-level studies.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 54.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. International Food Information Council. 2020 Food & Health Survey 2020 Available from: https://foodinsight.org/2020-food-and-health-survey/.

  2. Kamphuis CB, de Bekker-Grob EW, van Lenthe FJ. Factors affecting food choices of older adults from high and low socioeconomic groups: a discrete choice experiment. Am J Clin Nutr. 2015;101(4):768–74.

    Article  CAS  PubMed  Google Scholar 

  3. Kourouniotis S, Keast RSJ, Riddell LJ, Lacy K, Thorpe MG, Cicerale S. The importance of taste on dietary choice, behaviour and intake in a group of young adults. Appetite. 2016;103:1–7.

    Article  CAS  PubMed  Google Scholar 

  4. Duffy VB, Lucchina LA, Bartoshuk LM. Genetic variation in taste: potential biomarker for cardiovascular disease risk? In: Sensitivity to PROP (6-n-propylthiouracil): measurement, significances and implications. Erlangen: Merrkel Decker, Inc.; 2004.

    Google Scholar 

  5. Tucker RM, Kaiser KA, Parman MA, George BJ, Allison DB, Mattes RD. Comparisons of Fatty Acid Taste Detection Thresholds in People Who Are Lean vs. Overweight or Obese: A Systematic Review and Meta-Analysis. PLoS One. 2017;12(1):e0169583.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Lim J, Pullicin AJ. Oral carbohydrate sensing: beyond sweet taste. Physiol Behav. 2019;202:14–25.

    Article  CAS  PubMed  Google Scholar 

  7. Reyes MM, Gravina SA, Hayes JE. Evaluation of sweetener synergy in humans by Isobole analyses. Chem Senses. 2019;44(8):571–82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Hayes JE. Transdisciplinary perspectives on sweetness. Chemosens Percept. 2008;1(1):48–57.

    Article  Google Scholar 

  9. DuBois GE. Molecular mechanism of sweetness sensation. Physiol Behav. 2016;164(Pt B):453–63.

    Article  CAS  PubMed  Google Scholar 

  10. Behrens M, Briand L, de March CA, Matsunami H, Yamashita A, Meyerhof W, et al. Structure-function relationships of olfactory and taste receptors. Chem Senses. 2018;43(2):81–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Fierro F, Giorgetti A, Carloni P, Meyerhof W, Alfonso-Prieto M. Dual binding mode of "bitter sugars" to their human bitter taste receptor target. Sci Rep. 2019;9(1):8437.

    Article  PubMed  PubMed Central  Google Scholar 

  12. Dagan-Wiener A, Di Pizio A, Nissim I, Bahia MS, Dubovski N, Margulis E, et al. BitterDB: taste ligands and receptors database in 2019. Nucleic Acids Res. 2019;47(D1):D1179–D85.

    Article  PubMed  Google Scholar 

  13. Kim UK, Jorgenson E, Coon H, Leppert M, Risch N, Drayna D. Positional cloning of the human quantitative trait locus underlying taste sensitivity to phenylthiocarbamide. Science. 2003;299(5610):1221–5.

    Article  CAS  PubMed  Google Scholar 

  14. Reed DR, Zhu G, Breslin PA, Duke FF, Henders AK, Campbell MJ, et al. The perception of quinine taste intensity is associated with common genetic variants in a bitter receptor cluster on chromosome 12. Hum Mol Genet. 2010;19(21):4278–85.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Liman ER, Kinnamon SC. Sour taste: receptors, cells and circuits. Curr Opin Physiol. 2021;20:8–15.

    Article  PubMed  Google Scholar 

  16. Dias AG, Rousseau D, Duizer L, Cockburn M, Chiu W, Nielsen D, et al. Genetic variation in putative salt taste receptors and salt taste perception in humans. Chem Senses. 2013;38(2):137–45.

    Article  CAS  PubMed  Google Scholar 

  17. Bartoshuk LM, Duffy VB, Miller IJ Jr. PTC/PROP tasting: anatomy, psychophysics, and sex effects. Physiol Behav. 1994;56(6):1165–71.

    Article  CAS  PubMed  Google Scholar 

  18. Nolden AA, McGeary JE, Hayes JE. Predominant qualities evoked by quinine, sucrose, and capsaicin associate with PROP bitterness, but not TAS2R38 genotype. Chem Senses. 2020;45(5):383–90.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Bartoshuk L, Duffy V, Lucchina L, Prutkin J, Fast K. PROP (6-n-propylthiouracil) supertasters and the saltiness of NaCl. In: The XII International Symposium on Olfaction and Taste. San Diego: Annals of the New York Academy of Sciences; 1998.

    Google Scholar 

  20. Hayes JE, Keast RS. Two decades of supertasting: where do we stand? Physiol Behav. 2011;104(5):1072–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Kumari A, Ermilov AN, Grachtchouk M, Dlugosz AA, Allen BL, Bradley RM, et al. Recovery of taste organs and sensory function after severe loss from hedgehog/smoothened inhibition with cancer drug sonidegib. Proc Natl Acad Sci U S A. 2017;114(48):E10369–E78.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Mistretta CM, Kumari A. Hedgehog signaling regulates taste organs and oral sensation: distinctive roles in the epithelium, stroma, and innervation. Int J Mol Sci. 2019;20(6):1341.

    Article  CAS  PubMed Central  Google Scholar 

  23. Mastrangelo A, Bonato M, Cinque P. Smell and taste disorders in COVID-19: from pathogenesis to clinical features and outcomes. Neurosci Lett. 2021;748:135694.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Snyder DJ, Bartoshuk LM. Oral sensory nerve damage: causes and consequences. Rev Endocr Metab Disord. 2016;17(2):149–58.

    Article  PubMed  PubMed Central  Google Scholar 

  25. Bartoshuk LM, Gent J, Catalanotto FA, Goodspeed RB. Clinical evaluation of taste. Am J Otolaryngol. 1983;4(4):257–60.

    Article  CAS  PubMed  Google Scholar 

  26. Feeney EL, Hayes JE. Regional differences in suprathreshold intensity for bitter and umami stimuli. Chemosens Percept. 2014;7(3–4):147–57.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Running CA, Hayes JE. Sip and spit or sip and swallow: choice of method differentially alters taste intensity estimates across stimuli. Physiol Behav. 2017;181:95–9.

    Article  CAS  PubMed  Google Scholar 

  28. Miller I, Reedy F. Variation in human taste bud density and taste intensity perception. Physiol Behav. 1990;47:1213–9.

    Article  PubMed  Google Scholar 

  29. Bull TR. Taste and the chorda tympani. J Laryngol Otol. 1965;79:479–93.

    Article  CAS  PubMed  Google Scholar 

  30. Lehman CD, Bartoshuk LM, Catalanotto FC, Kveton JF, Lowlicht RA. Effect of anesthesia of the chorda tympani nerve of taste perception in humans. Physiol Behav. 1995;57:943–51.

    Article  CAS  PubMed  Google Scholar 

  31. Yanagisawa K, Bartoshuk LM, Catalanotto FA, Karrer TA, Kveton JF. Anesthesia of the chorda tympani nerve and taste phantoms. Physiol Behav. 1997;63(3):329–35.

    Article  Google Scholar 

  32. Sipiora M, Murtaugh M, Gregoire M, Duffy V. Bitter taste perception and severe vomiting during pregnancy. Physiol Behav. 2000;69(3):259–67.

    Article  CAS  PubMed  Google Scholar 

  33. Peracchio HL, Henebery KE, Sharafi M, Hayes JE, Duffy VB. Otitis media exposure associates with dietary preference and adiposity: a community-based observational study of at-risk preschoolers. Physiol Behav. 2012;106(2):264–71.

    Article  CAS  PubMed  Google Scholar 

  34. Rawal S, Huedo-Medina TB, Hoffman HJ, Swede H, Duffy VB. Structural equation modeling of associations among taste-related risk factors, taste functioning, and adiposity. Obesity (Silver Spring). 2017;25(4):781–7.

    Article  Google Scholar 

  35. Grushka M, Bartoshuk LM. Burning mouth syndrome and oral dysesthesias. Can J Diagnos. 2000:99–109.

    Google Scholar 

  36. Reed DR, Alhadeff AL, Beauchamp GK, Chaudhari N, Duffy VB, Dus M, et al. NIH workshop report: sensory nutrition and disease. Am J Clin Nutr. 2020:232–45.

    Google Scholar 

  37. Pucci A, Batterham RL. Endocrinology of the Gut and the Regulation of Body Weight and Metabolism. In: Feingold KR, Anawalt B, Boyce A, Chrousos G, de Herder WW, Dhatariya K, et al., editors. Endotext. South Dartmouth: MDText.com, Inc.; 2000.

    Google Scholar 

  38. Xie C, Wang X, Young RL, Horowitz M, Rayner CK, Wu T. Role of intestinal bitter sensing in enteroendocrine hormone secretion and metabolic control. Front Endocrinol. 2018;9:576.

    Article  Google Scholar 

  39. Sharafi M, Rawal S, Fernandez ML, Huedo-Medina TB, Duffy VB. Taste phenotype associates with cardiovascular disease risk factors via diet quality in multivariate modeling. Physiol Behav. 2018;194:103–12.

    Article  CAS  PubMed  Google Scholar 

  40. Calvert RD, Crawford CR, Running CA. The prevalence of improper solution-making technique places molar solutions in crisis. Res Food Sci Educ. 2020;19:183–91.

    Article  Google Scholar 

  41. Duffy VB, Peterson J, Bartoshuk LM. Associations between taste genetics, oral sensations and alcohol intake. Physiol Behav. 2004;82(2–3):435–45.

    Article  CAS  PubMed  Google Scholar 

  42. Watson AB, Pelli DG. QUEST: a Bayesian adaptive psychometric method. Percept Psychophys. 1983;33(2):113–20.

    Article  CAS  PubMed  Google Scholar 

  43. Hardikar S, Hochenberger R, Villringer A, Ohla K. Higher sensitivity to sweet and salty taste in obese compared to lean individuals. Appetite. 2017;111:158–65.

    Article  PubMed  Google Scholar 

  44. Mojet J, Christ-Hazelhof E, Heidema J. Taste perception with age: generic or specific losses in threshold sensitivity to the five basic tastes? Chem Senses. 2001;26(7):845–60.

    Article  CAS  PubMed  Google Scholar 

  45. Ogawa T, Uota M, Ikebe K, Arai Y, Kamide K, Gondo Y, et al. Longitudinal study of factors affecting taste sense decline in old-old individuals. J Oral Rehabil. 2017;44(1):22–9.

    Article  CAS  PubMed  Google Scholar 

  46. Mueller C, Kallert S, Renner B, Stiassny K, Temmel AF, Hummel T, et al. Quantitative assessment of gustatory function in a clinical context using impregnated "taste strips". Rhinology. 2003;41(1):2–6.

    CAS  PubMed  Google Scholar 

  47. Stillman JA, Morton RP, Hay KD, Ahmad Z, Goldsmith D. Electrogustometry: strengths, weaknesses, and clinical evidence of stimulus boundaries. Clin Otolaryngol Allied Sci. 2003;28(5):406–10.

    Article  CAS  PubMed  Google Scholar 

  48. Hayes JE, Bartoshuk LM, Kidd JR, Duffy VB. Supertasting and PROP bitterness depends on more than the TAS2R38 gene. Chem Senses. 2008;33(3):255–65.

    Article  CAS  PubMed  Google Scholar 

  49. McAuliffe WK, Meiselman HL. The roles of practice and correction in the categorization of sour and bitter taste qualities. Percept Psychophys. 1974;16(2):242–4.

    Article  Google Scholar 

  50. Hoffman HJ, Rawal S, Li CM, Duffy VB. New chemosensory component to the U.S. National Health and nutrition examination survey (NHANES), first-year results for measured olfactory dysfunction. Rev Endocr Metab Disord. 2016;17(2):221–40.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Zheng Y, Shen Y, Zhu Z, Hu H. Associations between cadmium exposure and taste and smell dysfunction: results from the National Health and Nutrition Examination Survey (NHANES), 2011–2014. Int J Environ Res Public Health. 2020;17(3):943.

    Article  CAS  PubMed Central  Google Scholar 

  52. Churnin I, Qazi J, Fermin CR, Wilson JH, Payne SC, Mattos JL. Association between olfactory and gustatory dysfunction and cognition in older adults. Am J Rhinol Allergy. 2019;33(2):170–7.

    Article  PubMed  Google Scholar 

  53. Hayes JE, Sullivan BS, Duffy VB. Explaining variability in sodium intake through oral sensory phenotype, salt sensation and liking. Physiol Behav. 2010;100(4):369–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Mattes RD, Kumanyika SK, Halpern BP. Salt taste responsiveness and preference among normotensive, prehypertensive and hypertensive adults. Chem Senses. 1983;8(1):27–40.

    Article  Google Scholar 

  55. Dinnella C, Monteleone E, Piochi M, Spinelli S, Prescott J, Pierguidi L, et al. Individual variation in PROP status, fungiform papillae density, and responsiveness to taste stimuli in a large population sample. Chem Senses. 2018;43(9):697–710.

    CAS  PubMed  Google Scholar 

  56. Tepper B, Seldner A. Sweet taste and intake of sweet foods in normal pregnancy and pregnancy complicated by gestational diabetes mellitus. Am J Clin Nutr. 1999;70(2):277–84.

    Article  CAS  PubMed  Google Scholar 

  57. Hayes JE, Duffy VB. Revisiting sugar-fat mixtures: sweetness and creaminess vary with phenotypic markers of oral sensation. Chem Senses. 2007;32(3):225–36.

    Article  CAS  PubMed  Google Scholar 

  58. Duffy VB, Rawal S, Park J, Brand MH, Sharafi M, Bolling BW. Characterizing and improving the sensory and hedonic responses to polyphenol-rich aronia berry juice. Appetite. 2016;107:116–25.

    Article  PubMed  PubMed Central  Google Scholar 

  59. Lawless HT, Malone GJ. Comparison of rating scales: sensitivity, replicates and relative measurement. J Sens Stud. 1986;1(2):155–74.

    Article  Google Scholar 

  60. Morgan CT. S. S. Stevens: the psychophysicist. Bull Psychon Soc. 1975;6(3):234–7.

    Article  Google Scholar 

  61. Stevens SS. Adaptation-level vs the relativity of judgment. Am J Psychol. 1958;71:633–46.

    Article  CAS  PubMed  Google Scholar 

  62. Bartoshuk LM. Comparing sensory experiences across individuals: recent psychophysical advances illuminate genetic variation in taste perception. Chem Senses. 2000;25(4):447–60.

    Article  CAS  PubMed  Google Scholar 

  63. Higgins MJ, Gipple JT, Hayes JE. Common bitter stimuli show differences in their temporal profiles before and after swallowing. Food Qual Prefer. 2021;87:104041.

    Article  Google Scholar 

  64. CDC. National Health and Nutrition Examination Survey (NHANES) Taste and Smell Examination Component Manual [Internet]. Centers for Disease Control and Prevention (CDC), National Center for Health Statistics (NCHS) 2013. Available from: https://www.cdc.gov/nchs/data/nhanes/nhanes_13_14/Taste_Smell.pdf.

  65. Coldwell S, Duffy V, Bartoshuk L, Griffith J, Hoffman H. The NIH Toolbox Brief Gustation Assessment Protocol (abstract). 2013; Abstracts from the 35th Annual Meeting of AChemS—Chem Senses:A114.

    Google Scholar 

  66. Allen AL, McGeary JE, Knopik VS, Hayes JE. Bitterness of the non-nutritive sweetener acesulfame potassium varies with polymorphisms in TAS2R9 and TAS2R31. Chem Senses. 2013;38(5):379–89.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  67. Antenucci RG, Hayes JE. Nonnutritive sweeteners are not supernormal stimuli. Int J Obes. 2015;39(2):254–9.

    Article  CAS  Google Scholar 

  68. Green B, Gelhard B. Salt as an oral irritant. Chem Senses. 1989;14(2):259–71.

    Article  CAS  Google Scholar 

  69. Nolden AA, Hayes JE. Perceptual qualities of ethanol depend on concentration, and variation in these percepts associates with drinking frequency. Chemosens Percept. 2015;8(3):149–57.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  70. Lanier SA, Hayes JE, Duffy VB. Sweet and bitter tastes of alcoholic beverages mediate alcohol intake in of-age undergraduates. Physiol Behav. 2005;83(5):821–31.

    Article  CAS  PubMed  Google Scholar 

  71. Dinehart ME, Hayes JE, Bartoshuk LM, Lanier SL, Duffy VB. Bitter taste markers explain variability in vegetable sweetness, bitterness, and intake. Physiol Behav. 2006;87(2):304–13.

    Article  CAS  PubMed  Google Scholar 

  72. Duffy VB, Hayes JE, Sharafi M. Interactions between retronasal olfaction and taste influence vegetable liking and consumption: a psychophysical investigation. J Agric Food Res. 2020;2 https://doi.org/10.1016/j.jafr.2020.100044.

  73. Bartoshuk LM, Duffy VB, Green BG, Hoffman HJ, Ko CW, Lucchina LA, et al. Valid across-group comparisons with labeled scales: the gLMS versus magnitude matching. Physiol Behav. 2004;82(1):109–14.

    Article  CAS  PubMed  Google Scholar 

  74. Green B, Shaffer G, Gilmore M. A semantically-labeled scale of oral sensation with apparent ratio properties. Chem Senses. 1993;18:683–702.

    Article  Google Scholar 

  75. Snyder DJ, Fast K, Bartoshuk LM. Valid comparisons of suprathreshold stimuli. J Conscious Stud. 2004;11:40–57.

    Google Scholar 

  76. Hayes JE, Allen AL, Bennett SM. Direct comparison of the generalized visual analog scale (gVAS) and general labeled magnitude scale (gLMS). Food Qual Prefer. 2013;28(1):36–44.

    Article  PubMed  Google Scholar 

  77. Kamen J, Pilgrim F, Gutman N, Kroll B. Interactions of suprathreshold taste stimuli. J Exp Psychol. 1961;62:348–56.

    Article  CAS  PubMed  Google Scholar 

  78. Barragan R, Coltell O, Portoles O, Asensio EM, Sorli JV, Ortega-Azorin C, et al. Bitter, Sweet, Salty, Sour and Umami Taste Perception Decreases with Age: Sex-Specific Analysis, Modulation by Genetic Variants and Taste-Preference Associations in 18 to 80 Year-Old Subjects. Nutrients. 2018;10(10):1539.

    Article  PubMed Central  Google Scholar 

  79. Coltell O, Sorli JV, Asensio EM, Fernandez-Carrion R, Barragan R, Ortega-Azorin C, et al. Association between taste perception and adiposity in overweight or obese older subjects with metabolic syndrome and identification of novel taste-related genes. Am J Clin Nutr. 2019;109(6):1709–23.

    Article  PubMed  Google Scholar 

  80. Parducci A. Perceptual and Judgement Relativity. In: Sarris V, Parducci A, editors. Perspectives in psychological experimentation: toward the year 2000. Hillsdale: Lawrence Erlbaum Associates; 1984. p. 370.

    Google Scholar 

  81. Snyder DJ, Prescott J, Bartoshuk LM. Modern psychophysics and the assessment of human oral sensation. Adv Otorhinolaryngol. 2006;63:221–41.

    PubMed  Google Scholar 

  82. Green B, Dalton P, Cowart B, Rankin K, Higgins J. Evaluation the labeled magnitude scale for measuring sensations of taste and smell. Chem Senses. 1996;21:323–34.

    Article  CAS  PubMed  Google Scholar 

  83. Webb J, Bolhuis DP, Cicerale S, Hayes JE, Keast R. The relationships between common measurements of taste function. Chemosens Percept. 2015;8(1):11–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  84. Karrer T, Bartoshuk L. Capsaicin desensitization and recovery on the human tongue. Physiol Behav. 1991;49:757–64.

    Article  CAS  PubMed  Google Scholar 

  85. Williams E. Experimental designs balanced for the estimation of residual effects of treatments. Aust J Sci Res. 1949;Ser A 2:149–68.

    Google Scholar 

  86. Nolden AA, Hayes JE. Perceptual and affective responses to sampled capsaicin differ by reported intake. Food Qual Prefer. 2017;55:26–34.

    Article  PubMed  Google Scholar 

  87. Moskowitz HR. The sweetness and pleasantness of sugars. Am J Psychol. 1971;84(3):387–405.

    Article  CAS  PubMed  Google Scholar 

  88. Iatridi V, Hayes JE, Yeomans MR. Reconsidering the classification of sweet taste liker phenotypes: a methodological review. Food Qual Prefer. 2019;72:56–76.

    Article  Google Scholar 

  89. Mennella JA, Lukasewycz LD, Griffith JW, Beauchamp GK. Evaluation of the Monell forced-choice, paired-comparison tracking procedure for determining sweet taste preferences across the lifespan. Chem Senses. 2011;36(4):345–55.

    Article  PubMed  PubMed Central  Google Scholar 

  90. Beauchamp G, Cowart B. Preference for high salt concentrations among children. Dev Psychol. 1990;26:539–45.

    Article  Google Scholar 

  91. Iatridi V, Hayes JE, Yeomans MR. Quantifying sweet taste liker phenotypes: time for some consistency in the classification criteria. Nutrients. 2019;11(1):129.

    Article  CAS  PubMed Central  Google Scholar 

  92. Lim J, Wood A, Green BG. Derivation and evaluation of a labeled hedonic scale. Chem Senses. 2009;34(9):739–51.

    Article  PubMed  PubMed Central  Google Scholar 

  93. Bartoshuk LM, Duffy VB, Hayes JE, Moskowitz HR, Snyder DJ. Psychophysics of sweet and fat perception in obesity: problems, solutions and new perspectives. Philos Trans R Soc Lond Ser B Biol Sci. 2006;361(1471):1137–48.

    Article  Google Scholar 

  94. Watson D. Clark LATP-XMftPaNAS-EFATUoI. The PANAS-X: manual for the positive and negative affect schedule-expanded form. Ames: The University of Iowa; 1994.

    Google Scholar 

  95. Shahbake M, Hutchinson I, Laing DG, Jinks AL. Rapid quantitative assessment of fungiform papillae density in the human tongue. Brain Res. 2005;1052(2):196–201.

    Article  CAS  PubMed  Google Scholar 

  96. Melis M, Atzori E, Cabras S, Zonza A, Calo C, Muroni P, et al. The gustin (CA6) gene polymorphism, rs2274333 (a/G), as a mechanistic link between PROP tasting and fungiform taste papilla density and maintenance. PLoS One. 2013;8(9):e74151.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  97. Saito T, Ito T, Ito Y, Manabe Y, Sano K. Relationship between gustatory function and average number of taste buds per fungiform papilla measured by confocal laser scanning microscopy in humans. Eur J Oral Sci. 2017;125(1):44–8.

    Article  CAS  PubMed  Google Scholar 

  98. Eldeghaidy S, Thomas D, Skinner M, Ford R, Giesbrecht T, Thomas A, et al. An automated method to detect and quantify fungiform papillae in the human tongue: validation and relationship to phenotypical differences in taste perception. Physiol Behav. 2018;184:226–34.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  99. Cruickshanks KJ, Schubert CR, Snyder DJ, Bartoshuk LM, Huang GH, Klein BE, et al. Measuring taste impairment in epidemiologic studies: the beaver dam offspring study. Ann N Y Acad Sci. 2009;1170:543–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  100. Fast K, Duffy V, Bartoshuk LM. New psychophysical insights in evaluating genetic variation in taste. In: Rouby C, Schaal B, Dubios D, Gervais R, Holley A, editors. Olfaction, taste and cognition. Cambridge: Cambridge University Press; 2002.

    Google Scholar 

  101. Duffy VB, Hayes JE, Davidson AC, Kidd JR, Kidd KK, Bartoshuk LM. Vegetable intake in college-aged adults is explained by oral sensory phenotypes and TAS2R38 genotype. Chemosens Percept. 2010;3(3–4):137–48.

    Article  PubMed  PubMed Central  Google Scholar 

  102. Sollai G, Melis M, Pani D, Cosseddu P, Usai I, Crnjar R, et al. First objective evaluation of taste sensitivity to 6-n-propylthiouracil (PROP), a paradigm gustatory stimulus in humans. Sci Rep. 2017;7:40353.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  103. Bartoshuk LM, Duffy VB, Chapo AK, Fast K, Yiee JH, Hoffman HJ, et al. From psychophysics to the clinic: missteps and advances. Food Qual Prefer. 2004;15:617–32.

    Article  Google Scholar 

  104. Drewnowski A, Kristal A, Cohen J. Genetic taste responses to 6-n-propylthiouracil among adults: a screening tool for epidemiological studies. Chem Senses. 2001;26(5):483–9.

    Article  CAS  PubMed  Google Scholar 

  105. Landis BN, Welge-Luessen A, Bramerson A, Bende M, Mueller CA, Nordin S, et al. "taste strips" - a rapid, lateralized, gustatory bedside identification test based on impregnated filter papers. J Neurol. 2009;256(2):242–8.

    Article  PubMed  Google Scholar 

  106. Kalveram L, Gohlisch J, Brauchmann J, Overberg J, Kuhnen P, Wiegand S. Gustatory function can improve after multimodal lifestyle intervention: a longitudinal observational study in pediatric patients with obesity. Child Obes. 2021;17(2):136–43.

    Article  PubMed  Google Scholar 

  107. Niklassen AS, Draf J, Huart C, Hintschich C, Bocksberger S, Trecca EMC, et al. COVID-19: recovery from chemosensory dysfunction. A multicentre study on smell and taste. Laryngoscope. 2021;131(5):1095–100.

    Article  CAS  PubMed  Google Scholar 

  108. Smutzer G, Lam S, Hastings L, Desai H, Abarintos RA, Sobel M, et al. A test for measuring gustatory function. Laryngoscope. 2008;118(8):1411–6.

    Article  PubMed  PubMed Central  Google Scholar 

  109. Desai H, Smutzer G, Coldwell SE, Griffith JW. Validation of edible taste strips for identifying PROP taste recognition thresholds. Laryngoscope. 2011;121(6):1177–83.

    Article  PubMed  PubMed Central  Google Scholar 

  110. Smutzer G, Patel JY, Stull JC, Abarintos RA, Khan NK, Park KC. A preference test for sweet taste that uses edible strips. Appetite. 2014;73:132–9.

    Article  PubMed  Google Scholar 

  111. Matthews JN, Altman DG, Campbell MJ, Royston P. Analysis of serial measurements in medical research. BMJ. 1990;300(6719):230–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  112. Duffy VB, Glennon SG, Larsen BA, Rawal S, Oncken C, Litt MD. Heightened olfactory dysfunction and oral irritation among chronic smokers and heightened propylthiouracil (PROP) bitterness among menthol smokers. Physiol Behav. 2019;15(201):111–22.

    Article  Google Scholar 

  113. Tepper BJ, Christensen CM, Cao J. Development of brief methods to classify individuals by PROP taster status. Physiol Behav. 2001;73(4):571–7.

    Article  CAS  PubMed  Google Scholar 

  114. Fischer ME, Cruickshanks KJ, Schubert CR, Pinto A, Huang GH, Klein BE, et al. The association of taste with change in adiposity-related health measures. J Acad Nutr Diet. 2014;114(8):1195–202.

    Article  PubMed  PubMed Central  Google Scholar 

  115. Thompson DA, Moskowitz HR, Campbell RG. Taste and olfaction in human obesity. Physiol Behav. 1977;19(2):335–7.

    Article  CAS  PubMed  Google Scholar 

  116. Iatridi V, Armitage RM, Yeomans MR, Hayes JE. Effects of sweet-liking on body composition depend on age and lifestyle: a challenge to the simple sweet-liking-obesity hypothesis. Nutrients. 2020;12(9):2072.

    Article  Google Scholar 

  117. Blanchard B, Larsen B, McCaffrey J, Duffy V. Sensory and Survey Designation of Sweet Liking Contribute to Diet Quality, Adiposity and Blood Pressure Relationships in Adults. In: Association for Chemoreception Sciences (AChemS) Meeting. Bonita Springs: Chem Senses; 2019.

    Google Scholar 

  118. Blicher B, Joshipura K, Eke P. Validation of self-reported periodontal disease: a systematic review. J Dent Res. 2005;84(10):881–90.

    Article  CAS  PubMed  Google Scholar 

  119. Lenderink AF, Zoer I, van der Molen HF, Spreeuwers D, Frings-Dresen MH, van Dijk FJ. Review on the validity of self-report to assess work-related diseases. Int Arch Occup Environ Health. 2012;85(3):229–51.

    Article  PubMed  Google Scholar 

  120. Rawal S, Hoffman HJ, Chapo AK, Duffy VB. Sensitivity and specificity of self-reported olfactory dysfunction in a home-based study of independent-living. Healthy older women. Chemosens Percept. 2014;7(304):108–16.

    Article  PubMed  PubMed Central  Google Scholar 

  121. Rawal S, Hoffman HJ, Honda M, Huedo-Medina TB, Duffy VB. The taste and smell protocol in the 2011-2014 U.S. National Health and nutrition examination survey (NHANES): test-retest reliability and validity testing. Chemosens Percept. 2015;8(3):138–48.

    Article  PubMed  PubMed Central  Google Scholar 

  122. Rawal S, Hoffman HJ, Bainbridge KE, Huedo-Medina TB, Duffy VB. Prevalence and risk factors of self-reported smell and taste alterations: results from the 2011-2012 US National Health and nutrition examination survey (NHANES). Chem Senses. 2016;41(1):69–76.

    Article  PubMed  Google Scholar 

  123. Hoffman H, Bartoshuk L, Losonczy K, Rawal S, Hayes JE, Li CM, Duffy VB, et al. Dysgeusia, Hypogeusia and Hypergeusia Among US Adults Aged >40 Years: The National Health and Nutrition Examination Survey (NHANES), 2011–2014 (abstract). Chem Senses. in press:2019.

    Google Scholar 

  124. Keskitalo K, Knaapila A, Kallela M, Palotie A, Wessman M, Sammalisto S, et al. Sweet taste preferences are partly genetically determined: identification of a trait locus on chromosome 16. Am J Clin Nutr. 2007;86(1):55–63.

    Article  CAS  PubMed  Google Scholar 

  125. Hayes JE, Wallace MR, Knopik VS, Herbstman DM, Bartoshuk LM, Duffy VB. Allelic variation in TAS2R bitter receptor genes associates with variation in sensations from and ingestive behaviors toward common bitter beverages in adults. Chem Senses. 2011;36(3):311–9.

    Article  CAS  PubMed  Google Scholar 

  126. Wanich U, Sayompark D, Riddell L, Cicerale S, Liem DG, Mohebbi M, et al. Assessing food liking: comparison of food liking questionnaires and direct food tasting in two cultures. Nutrients. 2018;10(12):463.

    Article  Google Scholar 

  127. Labonte ME, Kirkpatrick SI, Bell RC, Boucher BA, Csizmadi I, Koushik A, et al. Dietary assessment is a critical element of health research - perspective from the Partnership for Advancing Nutritional and Dietary Assessment in Canada. Appl Physiol Nutr Metab. 2016;41(10):1096–9.

    Article  PubMed  Google Scholar 

  128. Duffy VB, Hayes JE, Sullivan BS, Faghri P. Surveying food and beverage liking: a tool for epidemiological studies to connect chemosensation with health outcomes. Ann N Y Acad Sci. 2009;1170:558–68.

    Article  PubMed  Google Scholar 

  129. Lampure A, Castetbon K, Deglaire A, Schlich P, Peneau S, Hercberg S, et al. Associations between liking for fat, sweet or salt and obesity risk in French adults: a prospective cohort study. Int J Behav Nutr Phys Act. 2016;13:74.

    Article  PubMed  PubMed Central  Google Scholar 

  130. Lee YH, Shelley M, Liu CT, Chang YC. Assessing the association of food preferences and self-reported psychological well-being among middle-aged and older adults in contemporary china-results from the china health and nutrition survey. Int J Environ Res Public Health. 2018;15(3):463.

    Article  PubMed Central  Google Scholar 

  131. Shin Y, Lee S, Kim Y. Sweet preference associated with the risk of hypercholesterolemia among middle-aged women in Korea. J Atheroscler Thromb. 2018;25(12):1215–21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  132. Jilani H, Pohlabeln H, De Henauw S, Eiben G, Hunsberger M, Molnar D, et al. Relative validity of a food and beverage preference questionnaire to characterize taste phenotypes in children adolescents and adults. Nutrients. 2019;11(7):1453.

    Article  PubMed Central  Google Scholar 

  133. Fletcher S, Wright C, Jones A, Parkinson K, Adamson A. Tracking of toddler fruit and vegetable preferences to intake and adiposity later in childhood. Matern Child Nutr. 2017;13(2):e12290.

    Article  Google Scholar 

  134. Sharafi M, Perrachio H, Scarmo S, Huedo-Medina TB, Mayne ST, Cartmel B, et al. Preschool-adapted liking survey (PALS): a brief and valid method to assess dietary quality of preschoolers. Child Obes. 2015;11(5):530–40.

    Article  PubMed  Google Scholar 

  135. Duffy VB, Lanier SA, Hutchins HL, Pescatello LS, Johnson MK, Bartoshuk LM. Food preference questionnaire as a screening tool for assessing dietary risk of cardiovascular disease within health risk appraisals. J Am Diet Assoc. 2007;107(2):237–45.

    Article  PubMed  Google Scholar 

  136. Tuorila H, Huotilainen A, Lähteenmäki L, Ollila S, Tuomi-Nurmi S, Urala N. Comparison of affective rating scales and their relationship to variables reflecting food consumption. Food Quality Pref. 2008;19(1):51–61.

    Article  Google Scholar 

  137. Ramsay SA, Rudley M, Tonnemaker LE, Price WJ. A comparison of college Students' reported fruit and vegetable liking and intake from childhood to adulthood. J Am Coll Nutr. 2017;36(1):28–37.

    Article  PubMed  Google Scholar 

  138. Park H, Shin Y, Kwon O, Kim Y. Association of sensory liking for fat with dietary intake and metabolic syndrome in Korean adults. Nutrients. 2018;10(7):E877.

    Article  PubMed  Google Scholar 

  139. Charlot K, Malgoyre A, Bourrilhon C. Proposition for a shortened version of the Leeds food preference questionnaire (LFPQ). Physiol Behav. 2019;199:244–51.

    Article  CAS  PubMed  Google Scholar 

  140. Ledikwe JH, Ello-Martin J, Pelkman CL, Birch LL, Mannino ML, Rolls BJ. A reliable, valid questionnaire indicates that preference for dietary fat declines when following a reduced-fat diet. Appetite. 2007;49(1):74–83.

    Article  PubMed  Google Scholar 

  141. Sharafi M, Duffy VB, Miller RJ, Winchester SB, Sullivan MC. Dietary behaviors of adults born prematurely may explain future risk for cardiovascular disease. Appetite. 2016;99:157–67.

    Article  PubMed  PubMed Central  Google Scholar 

  142. Pallister T, Sharafi M, Lachance G, Pirastu N, Mohney RP, MacGregor A, et al. Food preference patterns in a UK twin cohort. Twin Res Hum Genet. 2015;18(6):793–805.

    Article  PubMed  Google Scholar 

  143. Krebs-Smith SM, Pannucci TE, Subar AF, Kirkpatrick SI, Lerman JL, Tooze JA, et al. Update of the healthy eating index: HEI-2015. J Acad Nutr Diet. 2018;118(9):1591–602.

    Article  PubMed  PubMed Central  Google Scholar 

  144. Vosburgh K, Smith SR, Oldman S, Huedo-Medina T, Duffy VB. Pediatric-adapted liking survey (PALS): a diet and activity screener in pediatric care. Nutrients. 2019;11(7):1641.

    Article  PubMed Central  Google Scholar 

  145. Xu R, Blanchard BE, McCaffrey JM, Woolley S, Corso LML, Duffy VB. Food liking-based diet quality indexes (dqi) generated by conceptual and machine learning explained variability in cardiometabolic risk factors in young adults. Nutrients. 2020;12(4):882.

    Article  PubMed Central  Google Scholar 

  146. Mattes R, Cowart B. Dietary assessment of patients with chemosensory disorders. J Am Diet Assoc. 1994;94(1):50–6.

    Article  CAS  PubMed  Google Scholar 

  147. Ferris AM, Duffy VB. The effect of olfactory deficits on nutritional status: Does age predict individuals at risk? In: Nutrition and the Chemical Senses in Aging: Recent Advances and Current Research Needs. Sarasota: NY Academy of Sciences; 1989.

    Google Scholar 

  148. Wilson MM, Thomas DR, Rubenstein LZ, Chibnall JT, Anderson S, Baxi A, et al. Appetite assessment: simple appetite questionnaire predicts weight loss in community-dwelling adults and nursing home residents. Am J Clin Nutr. 2005;82(5):1074–81.

    Article  CAS  PubMed  Google Scholar 

  149. Bainbridge KE, Byrd-Clark D, Leopold D. Factors associated with phantom odor perception among US adults: findings from the National Health and nutrition examination survey. JAMA Otolaryngol Head Neck Surg. 2018;144(9):807–14.

    Article  PubMed  PubMed Central  Google Scholar 

  150. Enzenbach C, Wicklein B, Wirkner K, Loeffler M. Evaluating selection bias in a population-based cohort study with low baseline participation: the LIFE-adult-study. BMC Med Res Methodol. 2019;19(1):135.

    Article  PubMed  PubMed Central  Google Scholar 

  151. Hoffman H, Ishii E, MacTurk R. Age-related changes in the prevalence of smell/taste problems among the United States adult population. Results of the 1994 disability supplement to the National Helath Interview Survey (NHIS). Ann N Y Acad Sci. 1998;855:716–22.

    Article  CAS  PubMed  Google Scholar 

  152. Coldwell SE, Mennella JA, Duffy VB, Pelchat ML, Griffith JW, Smutzer G, et al. Gustation assessment using the NIH toolbox. Neurology. 2013;80(11 Suppl 3):S20–4.

    Article  PubMed  PubMed Central  Google Scholar 

  153. Gupta V, Walia GK, Sachdeva MP. 'Mendelian randomization': an approach for exploring causal relations in epidemiology. Public Health. 2017;145:113–9.

    Article  CAS  PubMed  Google Scholar 

  154. Bouthoorn SH, van Lenthe FJ, Kiefte-de Jong JC, Taal HR, Wijtzes AI, Hofman A, et al. Genetic taste blindness to bitter and body composition in childhood: a mendelian randomization design. Int J Obes. 2014;38(7):1005–10.

    Article  CAS  Google Scholar 

  155. Sacerdote C, Guarrera S, Smith GD, Grioni S, Krogh V, Masala G, et al. Lactase persistence and bitter taste response: instrumental variables and mendelian randomization in epidemiologic studies of dietary factors and cancer risk. Am J Epidemiol. 2007;166(5):576–81.

    Article  PubMed  Google Scholar 

  156. 2020–2030 Strategic Plan for NIH Nutrition Research 2020 Available from: https://dpcpsi.nih.gov/onr/strategic-plan.

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Valerie Buzas Duffy .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2021 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Duffy, V.B., Rawal, S., Hayes, J.E. (2021). Measurement of Gustation: From Clinical to Population-Based Methods. In: Joseph, P.V., Duffy, V.B. (eds) Sensory Science and Chronic Diseases. Springer, Cham. https://doi.org/10.1007/978-3-030-86282-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-030-86282-4_4

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-86281-7

  • Online ISBN: 978-3-030-86282-4

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics